Next Article in Journal
Disrupted Homeostatic Cytokines Expression in Secondary Lymph Organs during HIV Infection
Next Article in Special Issue
Relevance of Endoplasmic Reticulum Stress Cell Signaling in Liver Cold Ischemia Reperfusion Injury
Previous Article in Journal
Antiallergic Phorbol Ester from the Seeds of Aquilaria malaccensis
Previous Article in Special Issue
Endoplasmic Reticulum Stress and Associated ROS
Article

C/EBP β Mediates Endoplasmic Reticulum Stress Regulated Inflammatory Response and Extracellular Matrix Degradation in LPS-Stimulated Human Periodontal Ligament Cells

1
State Key Laboratory of Military Stomatology, Department of Pediatric Dentistry, School of Stomatology, the Fourth Military Medical University, Xi’an 710032, China
2
Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi’an 710032, China
3
Department of Health Statistics, the Fourth Military Medical University, Xi’an 710033, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Masato Matsuoka
Int. J. Mol. Sci. 2016, 17(3), 385; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17030385
Received: 7 January 2016 / Revised: 2 March 2016 / Accepted: 2 March 2016 / Published: 22 March 2016
(This article belongs to the Special Issue Modulators of Endoplasmic Reticulum Stress)
Periodontitis is an oral inflammatory disease that not only affects the integrity of local tooth-supporting tissues but also impacts systemic health. A compositional shift in oral microbiota has been considered as the main cause of periodontitis; however, the potential mechanism has not been fully defined. Herein, we investigated the role of CCAAT/enhancer-binding protein β (C/EBP β), a member of the C/EBP family of transcription factors, in human periodontal ligament cells (hPDLCs) exposed to Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS). RT-PCR and Western blotting analysis showed that the expression of C/EBP β was significantly increased in hPDLCs stimulated with LPS stimuli. Overexpression of C/EBP β by the recombinant adenoviral vector pAd/C/EBP β markedly increased the expression of the pro-inflammatory cytokines IL-6 and IL-8, and matrix metalloproteinases (MMP)-8 and -9 in hPDLCs in response to LPS. Furthermore, the activation of endoplasmic reticulum (ER) stress was confirmed in LPS-stimulated hPDLCs by measuring the expression of the ER stress marker molecules protein kinase-like ER kinase (PERK), eIF2α, GRP78/Bip, and C/EBP homologous protein (CHOP). The ER stress inhibitor salubrinal repressed, but inducer tunicamycin enhanced, the production of IL-6, IL-8, MMP-8, and MMP-9 in hPDLCs. Additionally, ER stress inducer tunicamycin significantly increased the expression level of C/EBP β in hPDLCs. Blocking of C/EBP β by siRNA resulted in a significant decrease in the secretion of IL-6 and IL-8 and expression of MMP-8 and MMP-9 induced by tunicamycin treatment in hPDLCs. Taken together, ER stress appears to play a regulatory role in the inflammatory response and extracellular matrix (ECM) degradation in hPDLCs in response to LPS stimuli by activating C/EBP β expression. This enhances our understanding of human periodontitis pathology. View Full-Text
Keywords: periodontitis; C/EBP β; endoplasmic reticulum stress; human periodontal ligament cells; P. gingivalis LPS periodontitis; C/EBP β; endoplasmic reticulum stress; human periodontal ligament cells; P. gingivalis LPS
Show Figures

Graphical abstract

MDPI and ACS Style

Bai, Y.; Wei, Y.; Wu, L.; Wei, J.; Wang, X.; Bai, Y. C/EBP β Mediates Endoplasmic Reticulum Stress Regulated Inflammatory Response and Extracellular Matrix Degradation in LPS-Stimulated Human Periodontal Ligament Cells. Int. J. Mol. Sci. 2016, 17, 385. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17030385

AMA Style

Bai Y, Wei Y, Wu L, Wei J, Wang X, Bai Y. C/EBP β Mediates Endoplasmic Reticulum Stress Regulated Inflammatory Response and Extracellular Matrix Degradation in LPS-Stimulated Human Periodontal Ligament Cells. International Journal of Molecular Sciences. 2016; 17(3):385. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17030385

Chicago/Turabian Style

Bai, Yudi, Yi Wei, Lian Wu, Jianhua Wei, Xiaojing Wang, and Yuxiang Bai. 2016. "C/EBP β Mediates Endoplasmic Reticulum Stress Regulated Inflammatory Response and Extracellular Matrix Degradation in LPS-Stimulated Human Periodontal Ligament Cells" International Journal of Molecular Sciences 17, no. 3: 385. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17030385

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop