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Article

Confining a Protein-Containing Water Nanodroplet inside Silica Nanochannels

1
Dipartimento di Scienza e Alta Tecnologia and INSTM udr Como, Insubria University, Via Valleggio 9, I-22100 Como, Italy
2
Dipartimento di Chimica, Turin University, Via P. Giuria 7, I-10125 Turin, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(12), 2965; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20122965
Received: 21 May 2019 / Revised: 10 June 2019 / Accepted: 14 June 2019 / Published: 18 June 2019
Incorporation of biological systems in water nanodroplets has recently emerged as a new frontier to investigate structural changes of biomolecules, with perspective applications in ultra-fast drug delivery. We report on the molecular dynamics of the digestive protein Pepsin subjected to a double confinement. The double confinement stemmed from embedding the protein inside a water nanodroplet, which in turn was caged in a nanochannel mimicking the mesoporous silica SBA-15. The nano-bio-droplet, whose size fits with the pore diameter, behaved differently depending on the protonation state of the pore surface silanols. Neutral channel sections allowed for the droplet to flow, while deprotonated sections acted as anchoring piers for the droplet. Inside the droplet, the protein, not directly bonded to the surface, showed a behavior similar to that reported for bulk water solutions, indicating that double confinement should not alter its catalytic activity. Our results suggest that nanobiodroplets, recently fabricated in volatile environments, can be encapsulated and stored in mesoporous silicas. View Full-Text
Keywords: host–guest systems; protein confinement; molecular dynamics; mesoporous silica; water nanodroplets host–guest systems; protein confinement; molecular dynamics; mesoporous silica; water nanodroplets
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MDPI and ACS Style

Giussani, L.; Tabacchi, G.; Coluccia, S.; Fois, E. Confining a Protein-Containing Water Nanodroplet inside Silica Nanochannels. Int. J. Mol. Sci. 2019, 20, 2965. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20122965

AMA Style

Giussani L, Tabacchi G, Coluccia S, Fois E. Confining a Protein-Containing Water Nanodroplet inside Silica Nanochannels. International Journal of Molecular Sciences. 2019; 20(12):2965. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20122965

Chicago/Turabian Style

Giussani, Lara, Gloria Tabacchi, Salvatore Coluccia, and Ettore Fois. 2019. "Confining a Protein-Containing Water Nanodroplet inside Silica Nanochannels" International Journal of Molecular Sciences 20, no. 12: 2965. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20122965

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