Genome-Wide Analysis of Carboxylesterases (COEs) in the Whitefly, Bemisia tabaci (Gennadius)
Abstract
:1. Introduction
2. Results
2.1. Identification of COEs in the Genome of B. tabaci MED
2.2. Expression Profiling of B. tabaci MED COE Genes
2.3. Responses of COE Expression to Imidacloprid
2.4. COEs Involved in the Imidacloprid Resistance of B. tabaci MED
3. Discussion
4. Materials and Methods
4.1. Insect Strain
4.2. De Novo Identification of COE Genes
4.3. Phylogenetic Analysis of B. tabaci MED COE Genes
4.4. RNA-Seq Analysis
4.5. RNA Isolation and cDNA Synthesis
4.6. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) Analysis
4.7. dsRNA Synthesis and RNAi Assays
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
COE | Carboxylesterase |
RNAi | RNA interference |
FPKM | Fragments per kilobase of transcript per million fragments mapped |
NJ | Neighbor-joining |
JTT | Jones–Taylor–Thornton |
dsRNA | Double-strand RNA |
EGFP | Enhanced green fluorescent protein |
JH | Juvenile hormone |
JHE | Juvenile hormone esterase |
qRT-PCR | Quantitative real-time polymerase chain reaction |
AAAP | Amino acid/auxin permease |
P450 | Cytochrome P450 monooxygenase |
GST | Glutathione S-transferase |
ABC | ATP-binding cassette transporter |
mtCOI | Mitochondrial cytochrome oxidase I |
HMM | Hidden Markov model |
NCBI | National Center for Biotechnology Information |
EST | Expressed sequence tag |
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Class/Clades | B. mori | D. melanogaster | Ap. mellifera | An. gambiae | T. castaneum | A. pisum | B. tabaci |
---|---|---|---|---|---|---|---|
Intracellular catalytic class | |||||||
α-esterase | 55 | 13 | 8 | 16 | 26 | 5 | 6 |
secreted catalytic class | |||||||
JHE | 4 | 2 | 1 | 9 | 1 | 0 | 3 |
integument esterase | 2 | 3 | 1 | 0 | 2 | 0 | 0 |
β-esterase | 2 | 6 | 3 | 5 | 8 | 15 | 15 |
uncharacterized | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
glutactin | 0 | 4 | 0 | 9 | 0 | 0 | 1 |
neurodevelopmental class | |||||||
AChE | 2 | 1 | 2 | 2 | 2 | 2 | 4 |
uncharacterized | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
gliotactin | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
neuroligin | 6 | 4 | 5 | 5 | 4 | 3 | 10 |
neurotactin | 2 | 1 | 1 | 2 | 2 | 0 | 0 |
total | 76 | 37 | 24 | 51 | 48 | 28 | 42 |
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Xia, J.; Xu, H.; Yang, Z.; Pan, H.; Yang, X.; Guo, Z.; Yang, F.; Guo, L.; Sun, X.; Wang, S.; et al. Genome-Wide Analysis of Carboxylesterases (COEs) in the Whitefly, Bemisia tabaci (Gennadius). Int. J. Mol. Sci. 2019, 20, 4973. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20204973
Xia J, Xu H, Yang Z, Pan H, Yang X, Guo Z, Yang F, Guo L, Sun X, Wang S, et al. Genome-Wide Analysis of Carboxylesterases (COEs) in the Whitefly, Bemisia tabaci (Gennadius). International Journal of Molecular Sciences. 2019; 20(20):4973. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20204973
Chicago/Turabian StyleXia, Jixing, Haifeng Xu, Zezhong Yang, Huipeng Pan, Xin Yang, Zhaojiang Guo, Fengshan Yang, Litao Guo, Xiaodong Sun, Shaoli Wang, and et al. 2019. "Genome-Wide Analysis of Carboxylesterases (COEs) in the Whitefly, Bemisia tabaci (Gennadius)" International Journal of Molecular Sciences 20, no. 20: 4973. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20204973