Next Article in Journal
MiRNAs as Players in Rhabdomyosarcoma Development
Previous Article in Journal
HSF1Base: A Comprehensive Database of HSF1 (Heat Shock Factor 1) Target Genes
Previous Article in Special Issue
Neutrophil Maturation and Survival Is Controlled by IFN-Dependent Regulation of NAMPT Signaling
Article

In Vitro Effects of Vaspin on Porcine Granulosa Cell Proliferation, Cell Cycle Progression, and Apoptosis by Activation of GRP78 Receptor and Several Kinase Signaling Pathways Including MAP3/1, AKT, and STAT3

1
Department of Physiology and Toxicology of Reproduction, Institute of Zoology and Biomedical Research, Jagiellonian University in Krakow, 30-387 Krakow, Poland
2
Department of Experimental Hematology, Institute of Zoology and Biomedical Research, Jagiellonian University in Krakow, 30-387 Krakow, Poland
3
INRA, UMR85, Unité Physiologie de la Reproduction et des Comportements, F37380 Nouzilly, France
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(22), 5816; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20225816
Received: 30 September 2019 / Revised: 15 November 2019 / Accepted: 17 November 2019 / Published: 19 November 2019
Vaspin, a visceral adipose tissue-derived serine protease inhibitor, is expressed in the porcine ovary; it induces the activation of various kinases and steroidogenesis. The aim of this study was to examine the effect of vaspin on granulosa (Gc) proliferation, cell cycle regulation, and apoptosis. Porcine Gc was incubated with vaspin (0.01–10 ng/mL) for 24 to 72 h, proliferation was measured using alamarBlue assay, cell cycle progression was assessed using flow cytometry, and cyclin (D, E, and A) protein expression was measured using immunoblotting. Apoptosis was assessed by measuring caspase activity using Caspase-glo 3/7 assay. Furthermore, histone-associated DNA fragments levels were measured using a cell-death detection ELISA; BAX (bcl-2-like protein 4), BCL2 (B-cell lymphoma 2), caspases (-3, -8, and -9), p53 mRNA, and protein expression were assessed using real time PCR and immunoblotting. We found that vaspin significantly enhanced Gc proliferation and cell cycle progression into the S and G2/M phases and decreased apoptosis. We observed that siRNA silencing of the glucose-regulated protein (GRP78) receptor and pharmacological inhibitors of mitogen-activated kinase (MAP3/1/ERK1/2), Janus kinase (STAT3) and protein kinase B (AKT) blocked the ability of vaspin cell proliferation and enhanced caspase-3/7 activities. These results suggest that vaspin via mitogenic effect on porcine Gc acts as a new regulator of ovarian growth, development, or folliculogenesis. View Full-Text
Keywords: vaspin; proliferation; cell cycle; apoptosis; granulosa cells; ovary; pig vaspin; proliferation; cell cycle; apoptosis; granulosa cells; ovary; pig
Show Figures

Figure 1

MDPI and ACS Style

Kurowska, P.; Mlyczyńska, E.; Dawid, M.; Opydo-Chanek, M.; Dupont, J.; Rak, A. In Vitro Effects of Vaspin on Porcine Granulosa Cell Proliferation, Cell Cycle Progression, and Apoptosis by Activation of GRP78 Receptor and Several Kinase Signaling Pathways Including MAP3/1, AKT, and STAT3. Int. J. Mol. Sci. 2019, 20, 5816. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20225816

AMA Style

Kurowska P, Mlyczyńska E, Dawid M, Opydo-Chanek M, Dupont J, Rak A. In Vitro Effects of Vaspin on Porcine Granulosa Cell Proliferation, Cell Cycle Progression, and Apoptosis by Activation of GRP78 Receptor and Several Kinase Signaling Pathways Including MAP3/1, AKT, and STAT3. International Journal of Molecular Sciences. 2019; 20(22):5816. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20225816

Chicago/Turabian Style

Kurowska, Patrycja, Ewa Mlyczyńska, Monika Dawid, Małgorzata Opydo-Chanek, Joelle Dupont, and Agnieszka Rak. 2019. "In Vitro Effects of Vaspin on Porcine Granulosa Cell Proliferation, Cell Cycle Progression, and Apoptosis by Activation of GRP78 Receptor and Several Kinase Signaling Pathways Including MAP3/1, AKT, and STAT3" International Journal of Molecular Sciences 20, no. 22: 5816. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20225816

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop