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Article

Arabidopsis Class II Formins AtFH13 and AtFH14 Can Form Heterodimers but Exhibit Distinct Patterns of Cellular Localization

Department of Experimental Plant Biology, Faculty of Sciences, Charles University, Viničná 5, CZ 128 44 Prague, Czech Republic
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 348; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21010348
Received: 2 December 2019 / Revised: 30 December 2019 / Accepted: 3 January 2020 / Published: 5 January 2020
(This article belongs to the Special Issue Frontiers in the Actin Cytoskeleton)
Formins are evolutionarily conserved multi-domain proteins participating in the control of both actin and microtubule dynamics. Angiosperm formins form two evolutionarily distinct families, Class I and Class II, with class-specific domain layouts. The model plant Arabidopsis thaliana has 21 formin-encoding loci, including 10 Class II members. In this study, we analyze the subcellular localization of two A. thaliana Class II formins exhibiting typical domain organization, the so far uncharacterized formin AtFH13 (At5g58160) and its distant homolog AtFH14 (At1g31810), previously reported to bind microtubules. Fluorescent protein-tagged full length formins and their individual domains were transiently expressed in Nicotiana benthamiana leaves under the control of a constitutive promoter and their subcellular localization (including co-localization with cytoskeletal structures and the endoplasmic reticulum) was examined using confocal microscopy. While the two formins exhibit distinct and only partially overlapping localization patterns, they both associate with microtubules via the conserved formin homology 2 (FH2) domain and with the periphery of the endoplasmic reticulum, at least in part via the N-terminal PTEN (Phosphatase and Tensin)-like domain. Surprisingly, FH2 domains of AtFH13 and AtFH14 can form heterodimers in the yeast two-hybrid assay—a first case of potentially biologically relevant formin heterodimerization mediated solely by the FH2 domain. View Full-Text
Keywords: AtFH13; AtFH14; At5g58160; At1g31810; FH2 domain; class II formin; confocal laser scanning microscopy; PTEN-like domain AtFH13; AtFH14; At5g58160; At1g31810; FH2 domain; class II formin; confocal laser scanning microscopy; PTEN-like domain
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MDPI and ACS Style

Kollárová, E.; Baquero Forero, A.; Stillerová, L.; Přerostová, S.; Cvrčková, F. Arabidopsis Class II Formins AtFH13 and AtFH14 Can Form Heterodimers but Exhibit Distinct Patterns of Cellular Localization. Int. J. Mol. Sci. 2020, 21, 348. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21010348

AMA Style

Kollárová E, Baquero Forero A, Stillerová L, Přerostová S, Cvrčková F. Arabidopsis Class II Formins AtFH13 and AtFH14 Can Form Heterodimers but Exhibit Distinct Patterns of Cellular Localization. International Journal of Molecular Sciences. 2020; 21(1):348. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21010348

Chicago/Turabian Style

Kollárová, Eva, Anežka Baquero Forero, Lenka Stillerová, Sylva Přerostová, and Fatima Cvrčková. 2020. "Arabidopsis Class II Formins AtFH13 and AtFH14 Can Form Heterodimers but Exhibit Distinct Patterns of Cellular Localization" International Journal of Molecular Sciences 21, no. 1: 348. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21010348

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