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Article

Cell Adhesion Molecule 1 Contributes to Cell Survival in Crowded Epithelial Monolayers

Department of Pathology, Kindai University Faculty of Medicine, 377-2 Ohno-higashi, Osaka-sayama, Osaka 589-8511, Japan
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Int. J. Mol. Sci. 2020, 21(11), 4123; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21114123
Received: 11 May 2020 / Revised: 2 June 2020 / Accepted: 6 June 2020 / Published: 9 June 2020
(This article belongs to the Special Issue Epithelial Cells and Cancer: Victims, Villains or Heroes?)
When epithelial cells in vivo are stimulated to proliferate, they crowd and often grow in height. These processes are likely to implicate dynamic interactions among lateral membranous proteins, such as cell adhesion molecule 1 (CADM1), an immunoglobulin superfamily member. Pulmonary epithelial cell lines that express CADM1, named NCI-H441 and RLE-6TN, were grown to become overconfluent in the polarized 2D culture system, and were examined for the expression of CADM1. Western analyses showed that the CADM1 expression levels increased gradually up to 3 times in a cell density-dependent manner. Confocal microscopic observations revealed dense immunostaining for CADM1 on the lateral membrane. In the overconfluent monolayers, CADM1 knockdown was achieved by two methods using CADM1-targeting siRNA and an anti-CADM1 neutralizing antibody. Antibody treatment experiments were also done on 6 other epithelial cell lines expressing CADM1. The CADM1 expression levels were reduced roughly by half, in association with cell height decrease by half in 3 lines. TUNEL assays revealed that the CADM1 knockdown increased the proportion of TUNEL-positive apoptotic cells approximately 10 folds. Increased expression of CADM1 appeared to contribute to cell survival in crowded epithelial monolayers. View Full-Text
Keywords: adhesion molecule; CADM1; epithelial cell; lateral membrane; apoptosis; neutralizing antibody; electroporation adhesion molecule; CADM1; epithelial cell; lateral membrane; apoptosis; neutralizing antibody; electroporation
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MDPI and ACS Style

Hagiyama, M.; Kimura, R.; Yoneshige, A.; Inoue, T.; Otani, T.; Ito, A. Cell Adhesion Molecule 1 Contributes to Cell Survival in Crowded Epithelial Monolayers. Int. J. Mol. Sci. 2020, 21, 4123. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21114123

AMA Style

Hagiyama M, Kimura R, Yoneshige A, Inoue T, Otani T, Ito A. Cell Adhesion Molecule 1 Contributes to Cell Survival in Crowded Epithelial Monolayers. International Journal of Molecular Sciences. 2020; 21(11):4123. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21114123

Chicago/Turabian Style

Hagiyama, Man, Ryuichiro Kimura, Azusa Yoneshige, Takao Inoue, Tomoyuki Otani, and Akihiko Ito. 2020. "Cell Adhesion Molecule 1 Contributes to Cell Survival in Crowded Epithelial Monolayers" International Journal of Molecular Sciences 21, no. 11: 4123. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21114123

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