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Open AccessArticle

In Vitro Antagonistic Effect of Gut Bacteriota Isolated from Indigenous Honey Bees and Essential Oils against Paenibacillus Larvae

1
Department of Fruit Science, Viticulture and Enology, Faculty of Horticulture and Landscape Engineering, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, Slovakia
2
Department of Bioenergetics, Food Analysis and Microbiology, Institute of Food Technology and Nutrition, University of Rzeszow, Cwiklinskiej 1, 35-601 Rzeszow, Poland
3
Institute of Food and Environmental Hygiene, Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, K. Helmaņaiela 8, LV-3004 Jelgava, Latvia
4
Department of Plant Genetics and Breeding, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, Slovakia
5
Department of Food Technology of Plant Origin, Poznań University of Life Sciences, 31 Wojska Polskiego St., 60-624 Poznań, Poland
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(18), 6736; https://doi.org/10.3390/ijms21186736
Received: 27 August 2020 / Revised: 7 September 2020 / Accepted: 12 September 2020 / Published: 14 September 2020
(This article belongs to the Section Molecular Microbiology)
The aim of study was to isolate and identify the gut bacteria of Apis mellifera and to evaluate antagonistic effect of the bacteriota against Paenibacillus larvae, which causes American foulbrood (AFB) in honeybees. The dilution plating method was used for the quantification of selected microbial groups from digestive tract of bees, with an emphasis on the bacteriota of the bees’ intestines. Bacteria were identified using mass spectrometry (MALDI-TOF-MS Biotyper). Overall, five classes, 27 genera and 66 species of bacteria were identified. Genera Lactobacillus (10 species) and Bacillus (8 species) were the most abundant. Gram-negative bacteria were represented with 16 genera, whereas Gram-positive with 10 genera. Delftia acidovorans and Escherichia coli were the most abundant in the digestive tract of honey bee. Resistance to a selection of antimicrobials was assessed for the bacterial isolates from bee gut and confirmed against all antimicrobials included in the study, with the exception of cefepime. Lactobacillus spp., especially L. kunkeei, L. crispatus and L. acidophilus. showed the strongest antimicrobial activity against P. larvae, the causal pathogen of AFB. Antimicrobial activity of essential oils against isolated bacteria and two isolates of P. larvae were assessed. Application of a broad selection of plant essential oils indicated that Thymus vulgaris had the highest antimicrobial activity against P. larvae. View Full-Text
Keywords: Lactobacillus spp.; rectum; intestine; antimicrobial activity; antimicrobial resistance; essential oils Lactobacillus spp.; rectum; intestine; antimicrobial activity; antimicrobial resistance; essential oils
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MDPI and ACS Style

Kačániová, M.; Terentjeva, M.; Žiarovská, J.; Kowalczewski, P.Ł. In Vitro Antagonistic Effect of Gut Bacteriota Isolated from Indigenous Honey Bees and Essential Oils against Paenibacillus Larvae. Int. J. Mol. Sci. 2020, 21, 6736. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21186736

AMA Style

Kačániová M, Terentjeva M, Žiarovská J, Kowalczewski PŁ. In Vitro Antagonistic Effect of Gut Bacteriota Isolated from Indigenous Honey Bees and Essential Oils against Paenibacillus Larvae. International Journal of Molecular Sciences. 2020; 21(18):6736. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21186736

Chicago/Turabian Style

Kačániová, Miroslava; Terentjeva, Margarita; Žiarovská, Jana; Kowalczewski, Przemysław Ł. 2020. "In Vitro Antagonistic Effect of Gut Bacteriota Isolated from Indigenous Honey Bees and Essential Oils against Paenibacillus Larvae" Int. J. Mol. Sci. 21, no. 18: 6736. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21186736

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