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Article

The Soybean bZIP Transcription Factor Gene GmbZIP2 Confers Drought and Salt Resistances in Transgenic Plants

1
College of Agriculture, Yangtze University, Hubei Collaborative Innovation Center for Grain Industry, Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Jingzhou 434025, China
2
Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China
3
College of Agronomy, Jilin Agricultural University, Changchun 130118, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to the work.
Int. J. Mol. Sci. 2020, 21(2), 670; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21020670
Received: 10 December 2019 / Revised: 15 January 2020 / Accepted: 15 January 2020 / Published: 20 January 2020
(This article belongs to the Section Molecular Biology)
Abiotic stresses, such as drought and salt, are major environmental stresses, affecting plant growth and crop productivity. Plant bZIP transcription factors (bZIPs) confer stress resistances in harsh environments and play important roles in each phase of plant growth processes. In this research, 15 soybean bZIP family members were identified from drought-induced de novo transcriptomic sequences of soybean, which were unevenly distributed across 12 soybean chromosomes. Promoter analysis showed that these 15 genes were rich in ABRE, MYB and MYC cis-acting elements which were reported to be involved in abiotic stress responses. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that 15 GmbZIP genes could be induced by drought and salt stress. GmbZIP2 was significantly upregulated under stress conditions and thus was selected for further study. Subcellular localization analysis revealed that the GmbZIP2 protein was located in the cell nucleus. qRT-PCR results show that GmbZIP2 can be induced by multiple stresses. The overexpression of GmbZIP2 in Arabidopsis and soybean hairy roots could improve plant resistance to drought and salt stresses. The result of differential expression gene analysis shows that the overexpression of GmbZIP2 in soybean hairy roots could enhance the expression of the stress responsive genes GmMYB48, GmWD40, GmDHN15, GmGST1 and GmLEA. These results indicate that soybean bZIPs played pivotal roles in plant resistance to abiotic stresses. View Full-Text
Keywords: bZIP transcription factor; expression pattern; gene regulate; abiotic stress resistance; soybean bZIP transcription factor; expression pattern; gene regulate; abiotic stress resistance; soybean
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MDPI and ACS Style

Yang, Y.; Yu, T.-F.; Ma, J.; Chen, J.; Zhou, Y.-B.; Chen, M.; Ma, Y.-Z.; Wei, W.-L.; Xu, Z.-S. The Soybean bZIP Transcription Factor Gene GmbZIP2 Confers Drought and Salt Resistances in Transgenic Plants. Int. J. Mol. Sci. 2020, 21, 670. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21020670

AMA Style

Yang Y, Yu T-F, Ma J, Chen J, Zhou Y-B, Chen M, Ma Y-Z, Wei W-L, Xu Z-S. The Soybean bZIP Transcription Factor Gene GmbZIP2 Confers Drought and Salt Resistances in Transgenic Plants. International Journal of Molecular Sciences. 2020; 21(2):670. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21020670

Chicago/Turabian Style

Yang, Yan, Tai-Fei Yu, Jian Ma, Jun Chen, Yong-Bin Zhou, Ming Chen, You-Zhi Ma, Wen-Liang Wei, and Zhao-Shi Xu. 2020. "The Soybean bZIP Transcription Factor Gene GmbZIP2 Confers Drought and Salt Resistances in Transgenic Plants" International Journal of Molecular Sciences 21, no. 2: 670. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21020670

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