Blue Light Mediates Chloroplast Avoidance and Enhances Photoprotection of Vanilla Orchid
Abstract
:1. Introduction
2. Results
2.1. Blue Light Induces Chloroplast Movement in Vanilla Orchid
2.2. Effect of Light and Temperature on Chl F of Vanilla Orchid
2.3. Extended BL-Acclimation Duration Enhances Chl F Capacity
2.4. Effect of Blue Light Acclimation on the Growth of Vanilla Orchid
2.4.1. BL-Acclimation Enhances Growth and Induces Chloroplast Avoidance
2.4.2. BL-Acclimation Increases Antioxidant Capacity and Sugar Content
2.4.3. BL-Acclimation Vanilla Orchid Altered Gene Expression Patterns
2.5. BL-Acclimation Increases Photoprotection of Vanilla Orchid
2.5.1. BL-Acclimation Enhances Growth and Increases Chl F Capacity
2.5.2. Effect of BL-Acclimation on Antioxidative Enzymes and Sugar Content under High Irradiation
2.5.3. BL-Acclimation Alters Gene Expression Patterns under High Irradiation
3. Discussion
3.1. Blue Light Mediates Chloroplast Avoidance in Vanilla Orchid
3.2. Vanilla Orchid is Tolerant to Moderately High Temperature but Sensitive to High Irradiation
3.3. Impact of Blue Light Acclimation on Vanilla Growth
3.3.1. BL-Acclimation Causes Chloroplast Avoidance to Reduce Photoinhibition
3.3.2. BL-Acclimation Reduces ROS and Enhances Antioxidants
3.3.3. BL-Acclimation Increases Photosynthesis-Related Genes under High Light Stress
4. Materials and Methods
4.1. Plant Materials and Experimental Design
4.2. Chloroplast Movement Using SPAD Measurement
4.3. Measurement of Chlorophyll Fluorescence Parameters
4.4. Measurement of Antioxidant Enzyme Activities and Sugar Content
4.5. Gene Expression Analysis
4.6. Statistical Analyses
5. Conclusion and Perspective
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
BL | Blue light |
ML | Moderate light |
HL | High light |
PSI | Photosystem I |
PSII | Photosystem II |
Fv/Fm | Maximal quantum efficiency of PSⅡ photochemistry |
ETR | Electron transport rate |
NPQ | Nonphotochemical quenching |
qP | Photochemical quenching of fluorescence |
qN | Nonphotochemical quenching coefficient |
SOD | Superoxide dismutase |
CAT | Catalase |
ROS | Reactive oxygen species |
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Source | df | L3 | LM |
---|---|---|---|
Model | 29 | 0.0665 *** | 0.0204 *** |
BL | 5 | 0.3737 *** | 0.1057 *** |
Time | 4 | 0.0012 ns | 0.0062 ns |
BL*Time | 20 | 0.0027 ns | 0.0019 ns |
Error | 120 | 0.0035 | 0.0027 |
Total | 149 |
BL Day | Light | Fv/Fm | ETR | NPQ | qP | qN | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
0 day | 150 | 0.74 | a | 9.50 | a | 2.05 | b | 0.32 | a | 0.81 | b | |
500 | 0.75 | a | 7.08 | ab | 2.59 | ab | 0.08 | b | 0.85 | ab | ||
1000 | 0.74 | a | 5.35 | b | 2.82 | a | 0.03 | b | 0.88 | a | ||
4 day | 150 | 0.74 | a | 15.75 | a | 2.50 | a | 0.60 | a | 0.86 | b | |
500 | 0.73 | a | 16.97 | a | 3.06 | a | 0.22 | b | 0.90 | a | ||
1000 | 0.72 | a | 7.82 | b | 3.03 | a | 0.06 | c | 0.90 | a | ||
8 day | 150 | 0.70 | a | 19.62 | a | 2.13 | b | 0.65 | a | 0.81 | b | |
500 | 0.70 | a | 16.63 | a | 3.11 | a | 0.24 | b | 0.90 | a | ||
1000 | 0.72 | a | 16.25 | a | 3.36 | a | 0.13 | c | 0.91 | a | ||
12 day | 150 | 0.71 | a | 17.02 | a | 2.53 | b | 0.64 | a | 0.85 | b | |
500 | 0.71 | a | 16.08 | a | 3.14 | ab | 0.23 | b | 0.90 | a | ||
1000 | 0.68 | a | 10.32 | b | 3.30 | a | 0.08 | c | 0.92 | a | ||
df | ||||||||||||
BLday | 3 | ** | *** | * | *** | *** | ||||||
Light | 2 | n.s. | *** | *** | *** | *** | ||||||
BLday × Light | 6 | n.s. | * | n.s. | *** | n.s. |
Light | BL_Ac | df | F/vFm | ETR | NPQ | qP | qN | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
ML500 | BL | 0.59 | a | 7.03 | a | 2.03 | a | 0.36 | a | 0.87 | a | |
CK | 0.61 | a | 7.99 | a | 2.12 | a | 0.34 | a | 0.85 | a | ||
HL1000 | BL | 0.42 | a | 5.65 | a | 1.98 | a | 0.35 | a | 0.89 | a | |
CK | 0.08 | b | −0.56 | b | 0.58 | b | −0.34 | a | 0.81 | a | ||
Light | 1 | *** | * | * | * | n.s. | ||||||
BL_Ac | 1 | * | n.s. | n.s. | * | n.s. | ||||||
Light × BL_Ac | 1 | * | n.s. | * | * | n.s. |
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Ko, S.-S.; Jhong, C.-M.; Lin, Y.-J.; Wei, C.-Y.; Lee, J.-Y.; Shih, M.-C. Blue Light Mediates Chloroplast Avoidance and Enhances Photoprotection of Vanilla Orchid. Int. J. Mol. Sci. 2020, 21, 8022. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218022
Ko S-S, Jhong C-M, Lin Y-J, Wei C-Y, Lee J-Y, Shih M-C. Blue Light Mediates Chloroplast Avoidance and Enhances Photoprotection of Vanilla Orchid. International Journal of Molecular Sciences. 2020; 21(21):8022. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218022
Chicago/Turabian StyleKo, Swee-Suak, Chung-Min Jhong, Yi-Jyun Lin, Ching-Yu Wei, Ju-Yin Lee, and Ming-Che Shih. 2020. "Blue Light Mediates Chloroplast Avoidance and Enhances Photoprotection of Vanilla Orchid" International Journal of Molecular Sciences 21, no. 21: 8022. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218022