Processed Eggshell Membrane Powder Is a Promising Biomaterial for Use in Tissue Engineering
Abstract
:1. Introduction
2. Results
2.1. Moisture Absorption and Texture Properties of Collagen Cryo-Scaffold is Improved with PEP
2.2. Scaffold with and without PEP Differ in Structure Properties
2.3. Fibroblasts and Skeletal Muscle Cells Retain their Phenotypes in Both Types of Scaffold
3. Discussion
4. Materials and Methods
4.1. Preparation of Scaffolds
4.2. Moisture Absorption and Texture Properties
4.3. Scanning Electron Microscopy (SEM)
4.4. Cell Culturing in Scaffolds
4.5. Histology and Immunohistochemistry
4.6. Quantitative Real-Time PCR
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
PEP | Processed eggshell membrane powder |
ESM | Eggshell membrane |
ECM | Extracellular matrix |
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Physical Properties | 1 h Post-Swelling | 4 h Post-Swelling | SEM | p Values | ||||
---|---|---|---|---|---|---|---|---|
Collagen | PEP | Collagen | PEP | Scaffold (S) | Time (T) | S × T | ||
Height swollen, mm | 1.50c | 2.75a | 1.25d | 2.28b | 0.13 | 0.000 | 0.000 | 0.012 |
Height reduction following compression, % 1 | 16.4 | 17.3 | - | - | 1.34 | 0.756 | - | - |
PBS content, mg/mm scaffold | 81.2b | 123.4a | 61.4c | 80.0b | 4.80 | 0.000 | 0.000 | 0.000 |
Water-holding capacity, % 2 | 75.0 | 69.5 | 84.2 | 77.2 | 1.23 | 0.000 | 0.000 | 0.528 |
Hardness, N | 2.19b | 2.57b | 2.31b | 4.44a | 0.21 | 0.000 | 0.000 | 0.000 |
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Rønning, S.B.; Berg, R.S.; Høst, V.; Veiseth-Kent, E.; Wilhelmsen, C.R.; Haugen, E.; Suso, H.-P.; Barham, P.; Schmidt, R.; Pedersen, M.E. Processed Eggshell Membrane Powder Is a Promising Biomaterial for Use in Tissue Engineering. Int. J. Mol. Sci. 2020, 21, 8130. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218130
Rønning SB, Berg RS, Høst V, Veiseth-Kent E, Wilhelmsen CR, Haugen E, Suso H-P, Barham P, Schmidt R, Pedersen ME. Processed Eggshell Membrane Powder Is a Promising Biomaterial for Use in Tissue Engineering. International Journal of Molecular Sciences. 2020; 21(21):8130. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218130
Chicago/Turabian StyleRønning, Sissel B., Ragnhild S. Berg, Vibeke Høst, Eva Veiseth-Kent, Christian R. Wilhelmsen, Eirik Haugen, Henri-Pierre Suso, Paul Barham, Ralf Schmidt, and Mona E. Pedersen. 2020. "Processed Eggshell Membrane Powder Is a Promising Biomaterial for Use in Tissue Engineering" International Journal of Molecular Sciences 21, no. 21: 8130. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218130