9-PAHSA Prevents Mitochondrial Dysfunction and Increases the Viability of Steatotic Hepatocytes
Abstract
:1. Introduction
2. Results
2.1. Pretreatment with 9-PAHSA Prevented Mitochondrial Dysfunction in Steatotic Cells
2.2. 9-PAHSA Treatment Improved the Viability of the Steatotic Primary Murine Hepatocytes (PMH)
2.3. 9-PAHSA Treatment Reduced the Intracellular Lipid Accumulation in Steatotic Cells
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Primary Murine Hepatocyte Purification
4.3. Steatosis Induction
4.4. Red Oil O Staining and Quantification
4.5. 9-PAHSA Treatment
4.6. Cell Viability
4.7. Mitochondrial Respiration
4.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
NAFLD | Nonalcoholic fatty liver disease |
NASH | Nonalcoholic steatohepatitis |
9-PAHSA | Palmitic-acid-9-hydroxy-stearic-acid |
NAFL | Nonalcoholic fatty liver |
FAHFA | Branched fatty acid esters of hydroxyl fatty acid |
OA | Oleic acid |
FCCP | Phenylhydrazone |
OCR | Oxygen consumption rate |
HFD | High fat diet |
BSA | Bovine serum albumin |
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Schultz Moreira, A.R.; Rüschenbaum, S.; Schefczyk, S.; Hendgen-Cotta, U.; Rassaf, T.; Broering, R.; Hardtke-Wolenski, M.; Buitrago-Molina, L.E. 9-PAHSA Prevents Mitochondrial Dysfunction and Increases the Viability of Steatotic Hepatocytes. Int. J. Mol. Sci. 2020, 21, 8279. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218279
Schultz Moreira AR, Rüschenbaum S, Schefczyk S, Hendgen-Cotta U, Rassaf T, Broering R, Hardtke-Wolenski M, Buitrago-Molina LE. 9-PAHSA Prevents Mitochondrial Dysfunction and Increases the Viability of Steatotic Hepatocytes. International Journal of Molecular Sciences. 2020; 21(21):8279. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218279
Chicago/Turabian StyleSchultz Moreira, Adriana R., Sabrina Rüschenbaum, Stefan Schefczyk, Ulrike Hendgen-Cotta, Tienush Rassaf, Ruth Broering, Matthias Hardtke-Wolenski, and Laura Elisa Buitrago-Molina. 2020. "9-PAHSA Prevents Mitochondrial Dysfunction and Increases the Viability of Steatotic Hepatocytes" International Journal of Molecular Sciences 21, no. 21: 8279. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218279