Inactivation of Infectious Bacteria Using Nonthermal Biocompatible Plasma Cabinet Sterilizer
Abstract
:1. Introduction
2. Results
2.1. Physical Characterization and RONS Generation of DBD Plasma Cabinet Sterilizer
2.2. Effect of Six Chambers (Cabinet 1) Plasma Cabinet Treatment on Inactivation of Bacteria
2.3. Effect of Three-Chamber (cabinet 2) Plasma Cabinet Treatment on the Inactivation of Bacteria
2.4. In Silico Study
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains and Maintenance of Culture
4.2. Preparation of Bacterial Cell Suspension
4.3. Experimental Cabinet Sterilizer and Plasma Generation in Cabinet
4.4. Measurement of Reactive Oxygen and Nitrogen Species (RONS)
4.5. Colony-Forming unit Analysis
4.6. In Silico Study
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Parameters | Conditions |
---|---|
Voltage (Vrms, kV) | 4.16 |
Current (Irms, mA) | 13.01 |
Period (µs) | 36.20 |
Frequency (kHz) | 27.6 |
Energy (Duty) (J/sec) | 0.24 |
Species | Chamber | Inhibition% | Species | Chamber | Inhibition% |
---|---|---|---|---|---|
E. coli (Dilution104) | Control | 0 | S. aureus (Dilution 104) | Control | 0 |
No. 1 | ND | No. 1 | 99.8 ± 0.1 | ||
No. 2 | 99.8 ± 0.1 | No. 2 | 99.7 ± 0.1 | ||
No. 3 | ND | No. 3 | 99.4 ± 0.1 | ||
No. 4 | 99.8 ± 0.1 | No. 4 | 99.7 ± 0.1 | ||
No. 5 | ND | No. 5 | 99.7 ± 0.1 | ||
No. 6 | 98.9 ± 0.2 | No. 6 | 99.3 ± 0.1 | ||
E. coli (Dilution105) | Control | 0 | S. aureus (Dilution 105) | Control | 0 |
No.1 | ND | No. 1 | 99.7 ± 0.1 | ||
No. 2 | 99.8 ± 0.1 | No. 2 | 99.3 ± 0.1 | ||
No. 3 | ND | No. 3 | 99.4 ± 0.2 | ||
No. 4 | 99.6 ± 0.1 | No. 4 | 99.5 ± 0.1 | ||
No. 5 | ND | No. 5 | 98.9 ± 0.1 | ||
No. 6 | 99.6 ± 0.1 | No. 6 | 99.2 ± 0.2 | ||
E. coli (Dilution 106) | Control | 0 | S. aureus (Dilution 106) | Control | 0 |
No. 1 | ND | No. 1 | 99.7 ± 0.1 | ||
No. 2 | ND | No. 2 | 99.4 ± 0.1 | ||
No. 3 | ND | No. 3 | 99.2 ± 0.1 | ||
No. 4 | ND | No. 4 | 99.4 ± 0.1 | ||
No. 5 | ND | No. 5 | ND | ||
No. 6 | ND | No. 6 | 99.8 ± 0.1 |
Species | Chamber | Inhibition% | Species | Chamber | Inhibition% |
---|---|---|---|---|---|
E. coli (Dilution104) | Control | 0 | S. aureus (Dilution 104) | Control | 0 |
No. 1 | 99.5 ± 0.1 | No. 1 | 98.5 ± 0.1 | ||
No. 2 | 99.8 ± 0.1 | No. 2 | 97.1 ± 0.1 | ||
No. 3 | 99.4 ± 0.1 | No. 3 | 97.7 ± 0.1 | ||
E. coli (Dilution105) | Control | 0 | S. aureus (Dilution 105) | Control | 0 |
No. 1 | 97.9 ± 0.1 | No. 1 | 99.6 ± 0.1 | ||
No. 2 | 99.5 ± 0.1 | No. 2 | 97.6 ± 0.1 | ||
No. 3 | 99.2 ± 0.1 | No. 3 | 98.8 ±0.1 | ||
E. coli (Dilution106) | Control | 0 | S. aureus (Dilution 106) | Control | 0 |
No. 1 | ND | No. 1 | ND | ||
No. 2 | ND | No. 2 | ND | ||
No. 3 | ND | No. 3 | ND |
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Akter, M.; Yadav, D.K.; Ki, S.H.; Choi, E.H.; Han, I. Inactivation of Infectious Bacteria Using Nonthermal Biocompatible Plasma Cabinet Sterilizer. Int. J. Mol. Sci. 2020, 21, 8321. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218321
Akter M, Yadav DK, Ki SH, Choi EH, Han I. Inactivation of Infectious Bacteria Using Nonthermal Biocompatible Plasma Cabinet Sterilizer. International Journal of Molecular Sciences. 2020; 21(21):8321. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218321
Chicago/Turabian StyleAkter, Mahmuda, Dharmendra Kumar Yadav, Se Hoon Ki, Eun Ha Choi, and Ihn Han. 2020. "Inactivation of Infectious Bacteria Using Nonthermal Biocompatible Plasma Cabinet Sterilizer" International Journal of Molecular Sciences 21, no. 21: 8321. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218321