Next Article in Journal
Application of Mesenchymal Stem Cells in Inflammatory and Fibrotic Diseases
Next Article in Special Issue
Transcriptome Analysis of Pyrus betulaefolia Seedling Root Responses to Short-Term Potassium Deficiency
Previous Article in Journal
Cardiovascular Active Peptides of Marine Origin with ACE Inhibitory Activities: Potential Role as Anti-Hypertensive Drugs and in Prevention of SARS-CoV-2 Infection
Previous Article in Special Issue
Calcium Plays a Double-Edged Role in Modulating Cadmium Uptake and Translocation in Rice
Article

Genome-Wide Analysis of the Five Phosphate Transporter Families in Camelina sativa and Their Expressions in Response to Low-P

1
Department of Plant and Microbial Biology, University of California at Berkeley, Berkeley, CA 94720, USA
2
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(21), 8365; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218365
Received: 22 October 2020 / Revised: 5 November 2020 / Accepted: 5 November 2020 / Published: 7 November 2020
(This article belongs to the Special Issue Ion Transport and Homeostasis in Plants)
Phosphate transporters (PHTs) play pivotal roles in phosphate (Pi) acquisition from the soil and distribution throughout a plant. However, there is no comprehensive genomic analysis of the PHT families in Camelina sativa, an emerging oilseed crop. In this study, we identified 73 CsPHT members belonging to the five major PHT families. A whole-genome triplication event was the major driving force for CsPHT expansion, with three homoeologs for each Arabidopsis ortholog. In addition, tandem gene duplications on chromosome 11, 18 and 20 further enlarged the CsPHT1 family beyond the ploidy norm. Phylogenetic analysis showed clustering of the CsPHT1 and CsPHT4 family members into four distinct groups, while CsPHT3s and CsPHT5s were clustered into two distinct groups. Promoter analysis revealed widespread cis-elements for low-P response (P1BS) specifically in CsPHT1s, consistent with their function in Pi acquisition and translocation. In silico RNA-seq analysis revealed more ubiquitous expression of several CsPHT1 genes in various tissues, whereas CsPHT2s and CsPHT4s displayed preferential expression in leaves. While several CsPHT3s were expressed in germinating seeds, most CsPHT5s were expressed in floral and seed organs. Suneson, a popular Camelina variety, displayed better tolerance to low-P than another variety, CS-CROO, which could be attributed to the higher expression of several CsPHT1/3/4/5 family genes in shoots and roots. This study represents the first effort in characterizing CsPHT transporters in Camelina, a promising polyploid oilseed crop that is highly tolerant to abiotic stress and low-nutrient status, and may populate marginal soils for biofuel production. View Full-Text
Keywords: PHT gene family; phylogenetic analysis; chromosomal distribution; protein domain; cis-element; tissue expression; low-P response; Camelina (false flax) PHT gene family; phylogenetic analysis; chromosomal distribution; protein domain; cis-element; tissue expression; low-P response; Camelina (false flax)
Show Figures

Figure 1

MDPI and ACS Style

Lhamo, D.; Shao, Q.; Tang, R.; Luan, S. Genome-Wide Analysis of the Five Phosphate Transporter Families in Camelina sativa and Their Expressions in Response to Low-P. Int. J. Mol. Sci. 2020, 21, 8365. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218365

AMA Style

Lhamo D, Shao Q, Tang R, Luan S. Genome-Wide Analysis of the Five Phosphate Transporter Families in Camelina sativa and Their Expressions in Response to Low-P. International Journal of Molecular Sciences. 2020; 21(21):8365. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218365

Chicago/Turabian Style

Lhamo, Dhondup, Qiaolin Shao, Renjie Tang, and Sheng Luan. 2020. "Genome-Wide Analysis of the Five Phosphate Transporter Families in Camelina sativa and Their Expressions in Response to Low-P" International Journal of Molecular Sciences 21, no. 21: 8365. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218365

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop