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Article

Dynamic Transcriptome Analysis of Anther Response to Heat Stress during Anthesis in Thermotolerant Rice (Oryza sativa L.)

Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(3), 1155; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031155
Received: 13 January 2020 / Revised: 6 February 2020 / Accepted: 7 February 2020 / Published: 10 February 2020
(This article belongs to the Special Issue Molecular Research in Rice: Agronomically Important Traits)
High temperature at anthesis is one of the most serious stress factors for rice (Oryza sativa L.) production, causing irreversible yield losses and reduces grain quality. Illustration of thermotolerance mechanism is of great importance to accelerate rice breeding aimed at thermotolerance improvement. Here, we identified a new thermotolerant germplasm, SDWG005. Microscopical analysis found that stable anther structure of SDWG005 under stress may contribute to its thermotolerance. Dynamic transcriptomic analysis totally identified 3559 differentially expressed genes (DEGs) in SDWG005 anthers at anthesis under heat treatments, including 477, 869, 2335, and 2210 for 1, 2, 6, and 12 h, respectively; however, only 131 were regulated across all four-time-points. The DEGs were divided into nine clusters according to their expressions in these heat treatments. Further analysis indicated that some main gene categories involved in heat-response of SDWG005 anthers, such as transcription factors, nucleic acid and protein metabolisms related genes, etc. Comparison with previous studies indicates that a core gene-set may exist for thermotolerance mechanism. Expression and polymorphic analysis of agmatine-coumarin-acyltransferase gene OsACT in different accessions suggested that it may involve in SDWG005 thermotolerance. This study improves our understanding of thermotolerance mechanisms in rice anthers during anthesis, and also lays foundation for breeding thermotolerant varieties via molecular breeding. View Full-Text
Keywords: rice; heat stress; transcriptome; anther; anthesis rice; heat stress; transcriptome; anther; anthesis
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MDPI and ACS Style

Liu, G.; Zha, Z.; Cai, H.; Qin, D.; Jia, H.; Liu, C.; Qiu, D.; Zhang, Z.; Wan, Z.; Yang, Y.; Wan, B.; You, A.; Jiao, C. Dynamic Transcriptome Analysis of Anther Response to Heat Stress during Anthesis in Thermotolerant Rice (Oryza sativa L.). Int. J. Mol. Sci. 2020, 21, 1155. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031155

AMA Style

Liu G, Zha Z, Cai H, Qin D, Jia H, Liu C, Qiu D, Zhang Z, Wan Z, Yang Y, Wan B, You A, Jiao C. Dynamic Transcriptome Analysis of Anther Response to Heat Stress during Anthesis in Thermotolerant Rice (Oryza sativa L.). International Journal of Molecular Sciences. 2020; 21(3):1155. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031155

Chicago/Turabian Style

Liu, Gang, Zhongping Zha, Haiya Cai, Dandan Qin, Haitao Jia, Changyan Liu, Dongfeng Qiu, Zaijun Zhang, Zhenghuang Wan, Yuanyuan Yang, Bingliang Wan, Aiqing You, and Chunhai Jiao. 2020. "Dynamic Transcriptome Analysis of Anther Response to Heat Stress during Anthesis in Thermotolerant Rice (Oryza sativa L.)" International Journal of Molecular Sciences 21, no. 3: 1155. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031155

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