Proregenerative Activity of IL-33 in Gastric Tissue Cells Undergoing Helicobacter Pylori-Induced Apoptosis

Round 1

Reviewer 1 Report

The authors present and interesting topic here on Proregenerative Activity of IL-33 in Gastric Tissue Cells Undergoing Helicobacter Pylori-Induced Apoptosis. A well conducted research. Issues which will need to be addressed are:

Abstract look ok.

Introduction: would be better if you can show a diagram regarding the pathogenesis or mechanism or gastric mucosa and IL-33

Materials and methods section should be before results and discussion

In methods section please explain in confounders to the study and how they were resolved.

Discussion section:

Explain the limitations of the study

Conclusion section should be included

Figures and tables look ok

English and grammar needs to be thoroughly checked throughout the manuscript

Author Response

Reviewer 1

R1. “Introduction: would be better if you can show a diagram regarding the pathogenesis or mechanism or …. gastric mucosa and IL-33”

Responses: Table 1 summarizing major IL-33 activities and Fig 7 showing the possible mechanisms of IL-33 role in H. pylori induced pathogenesis were included in the Introduction and Conclusions sections, respectively.

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R1. Materials and methods section should be before results and discussion.

Responses: Materials and methods section is right after Discussion and before Conclusions according to the journal requirements

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R1: In methods section please explain in confounders to the study and how they were resolved

Response: Potential confounders to the study were associated with methodology and were resolved in preliminary study

R1: Discussion section: Explain the limitations of the study

Response: In the section Results and Discussion 2.4 Control of H. pylori-induced Erk activation by IL-33.

The limitation of these data is associated with missing the IL-33 dependent molecular mechanism of Erk control, including the influence of well-defined H. pylori components and the inflammatory milieu. Further study in this area can help to better understand these processes.

Add in 243 line

R1: Conclusion section should be included

Response: Conclusion section was included to the text

Add in 654 line

R1: English and grammar needs to be thoroughly checked throughout the manuscript

Response: The linguistic correction of the manuscript was performed, and the text was revised. We also include the language certificate made by American Journal Experts PDF documents

Author Response File: Author Response.pdf

Reviewer 2 Report

The presented manuscript “Proregenerative Activity of IL-33 in Gastric Tissue Cells Undergoing Helicobacter Pylori-Induced Apoptosis” is an interesting topic and could be useful to understand the role of IL-33 in H pylori infection. The manuscript is well written and discussed. Though, I have a few minor comments.

• Section 2 results and discussion. TNF-alpha data has not shown to support the sentence " In the present study, primary guinea pig gastric epithelial cells and fibroblasts that were untransfected or transfected with IL-33 siRNA did not produce TNF-α in response to stimulation with H. pylori GE”
• The statistics in figure 3A ii, C i, ii is confusing. How the two different stars (single and double stars) represent two bars? please clarify it.
• Figure 2A please replace “contriol” with control. Please also complete the text “Guinea pig primary gastric cells of”?
• Figure 4A, please replace “epothelial” with “epithelial”.

Author Response

Reviewer 2.

R2: Section 2 results and discussion. TNF-alpha data has not shown to support the sentence “In the present study, primary guinea pig gastric epithelial cells and fibroblasts that were untransfected or transfected with IL-33 siRNA did not produce TNF-α in response to stimulation with H. pylori GE”

Response: This section of Results and Discussion was revised to support and explain the observation

R2: The statistics in figure 3A ii, C i, ii is confusing. How the two different starts (single and double stars) represent two bars? Please clarify it.

Response: The explanation of statistic in Figure 3 and following figures was included in figure legends

R2: Figure 2A please replace” contriol” with control. Please also complete the text “Guinea pig primary gastric cells of”?

Response: Misspelled word was corrected, and the part of text completed

R2: Figure 4A, please replace “epothelial with “epithelial”

Response: Misspelled word in the text was corrected

Language Certificate was add as a PDF file

Author Response File: Author Response.pdf