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Article

The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes

1
Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic
2
Department of Zoology, Faculty of Science, Charles University, BIOCEV, Vinicna 7, 128 44 Prague 2, Czech Republic
3
Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, University of Life Sciences Prague, Kamycka 129, 165 00 Prague 6, Czech Republic
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(7), 2651; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072651
Received: 27 February 2020 / Revised: 7 April 2020 / Accepted: 9 April 2020 / Published: 10 April 2020
(This article belongs to the Special Issue Advances in Molecular Regulation of Spermatozoa Function)
Fertilization is a multiple step process leading to the fusion of female and male gametes and the formation of a zygote. Besides direct gamete membrane interaction via binding receptors localized on both oocyte and sperm surface, fertilization also involves gamete communication via chemical molecules triggering various signaling pathways. This work focuses on a mouse taste receptor, mTAS1R3, encoded by the Tas1r3 gene, as a potential receptor mediating chemical communication between gametes using the C57BL/6J lab mouse strain. In order to specify the role of mTAS1R3, we aimed to characterize its precise localization in testis and sperm using super resolution microscopy. The testis cryo-section, acrosome-intact sperm released from cauda epididymis and sperm which underwent the acrosome reaction (AR) were evaluated. The mTAS1R3 receptor was detected in late spermatids where the acrosome was being formed and in the acrosomal cap of acrosome intact sperm. AR is triggered in mice during sperm maturation in the female reproductive tract and by passing through the egg surroundings such as cumulus oophorus cells. This AR onset is independent of the extracellular matrix of the oocyte called zona pellucida. After AR, the relocation of mTAS1R3 to the equatorial segment was observed and the receptor remained exposed to the outer surroundings of the female reproductive tract, where its physiological ligand, the amino acid L-glutamate, naturally occurs. Therefore, we targeted the possible interaction in vitro between the mTAS1R3 and L-glutamate as a part of chemical communication between sperm and egg and used an anti-mTAS1R3-specific antibody to block it. We detected that the acrosome reacted spermatozoa showed a chemotactic response in the presence of L-glutamate during and after the AR, and it is likely that mTAS1R3 acted as its mediator. View Full-Text
Keywords: acrosome reaction; chemotaxis; chemoattractant; gamete; L-glutamate; mouse; sperm; TAS1R family; mTAS1R3 receptor acrosome reaction; chemotaxis; chemoattractant; gamete; L-glutamate; mouse; sperm; TAS1R family; mTAS1R3 receptor
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MDPI and ACS Style

Frolikova, M.; Otcenaskova, T.; Valasková, E.; Postlerova, P.; Stopkova, R.; Stopka, P.; Komrskova, K. The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes. Int. J. Mol. Sci. 2020, 21, 2651. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072651

AMA Style

Frolikova M, Otcenaskova T, Valasková E, Postlerova P, Stopkova R, Stopka P, Komrskova K. The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes. International Journal of Molecular Sciences. 2020; 21(7):2651. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072651

Chicago/Turabian Style

Frolikova, Michaela; Otcenaskova, Tereza; Valasková, Eliska; Postlerova, Pavla; Stopkova, Romana; Stopka, Pavel; Komrskova, Katerina. 2020. "The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes" Int. J. Mol. Sci. 21, no. 7: 2651. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072651

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