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Interleukin-17 Reduces βENaC via MAPK Signaling in Vascular Smooth Muscle Cells

Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS 39206, USA
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Int. J. Mol. Sci. 2020, 21(8), 2953; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21082953
Received: 27 March 2020 / Revised: 8 April 2020 / Accepted: 14 April 2020 / Published: 22 April 2020
(This article belongs to the Section Molecular Biology)
Degenerin proteins, such as the beta epithelial Na+ channel (βENaC), are essential in the intracellular signaling of pressure-induced constriction, an important vascular smooth muscle cell (VSMC) function. While certain cytokines reduce ENaC protein in epithelial tissue, it is unknown if interleukin-17 (IL-17), a potent pro-inflammatory cytokine, directly mediates changes in membrane-associated βENaC in VSMCs. Therefore, we tested the hypothesis that exposure to IL-17 reduces βENaC in VSMCs through canonical mitogen-activated protein kinase (MAPK) signaling pathways. We treated cultured rat VSMCs (A10 cell line) with IL-17 (1–100 ng/mL) for 15 min to 16 h and measured expression of βENaC, p38MAPK, c-jun kinase (JNK), and nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB). IL-17 reduced βENaC protein expression in a concentration-dependent fashion and increased phosphorylation of p38MAPK by 15 min and JNK by 8 h. NFκB was unaffected by IL-17 in VSMCs. IL-17 treatment reduced VSMC viability but had no effect on cell death. To determine the underlying signaling pathway involved in this response, VSMCs were treated before and during IL-17 exposure with p38MAPK or JNK inhibitors. We found that JNK blockade prevented IL-17-mediated βENaC protein suppression. These data demonstrate that the pro-inflammatory cytokine IL-17 regulates VSMC βENaC via canonical MAPK signaling pathways, raising the possibility that βENaC-mediated loss of VSMC function may occur in inflammatory disorders. View Full-Text
Keywords: IL-17; degenerins; beta-ENaC; VSMCs; p38MAPK; JNK; NFκB IL-17; degenerins; beta-ENaC; VSMCs; p38MAPK; JNK; NFκB
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MDPI and ACS Style

Duncan, J.W.; Granger, J.P.; Ryan, M.J.; Drummond, H.A. Interleukin-17 Reduces βENaC via MAPK Signaling in Vascular Smooth Muscle Cells. Int. J. Mol. Sci. 2020, 21, 2953. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21082953

AMA Style

Duncan JW, Granger JP, Ryan MJ, Drummond HA. Interleukin-17 Reduces βENaC via MAPK Signaling in Vascular Smooth Muscle Cells. International Journal of Molecular Sciences. 2020; 21(8):2953. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21082953

Chicago/Turabian Style

Duncan, Jeremy W., Joey P. Granger, Michael J. Ryan, and Heather A. Drummond. 2020. "Interleukin-17 Reduces βENaC via MAPK Signaling in Vascular Smooth Muscle Cells" International Journal of Molecular Sciences 21, no. 8: 2953. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21082953

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