Next Article in Journal
Increase of the Intracellular Zinc Concentration Leads to an Activation and Internalisation of the Epidermal Growth Factor Receptor in A549 Cells
Next Article in Special Issue
Biomineralization of Collagen-Based Materials for Hard Tissue Repair
Previous Article in Journal
Role of Host-Mediated Post-Translational Modifications (PTMs) in RNA Virus Pathogenesis
Previous Article in Special Issue
Spatio-Temporal Bone Remodeling after Hematopoietic Stem Cell Transplantation
Article

CaSR-Mediated hBMSCs Activity Modulation: Additional Coupling Mechanism in Bone Remodeling Compartment

1
College of Life Science and Graduate School of Biotechnology, Kyung Hee University, Seochon-dong, Kiheung-go, Yongin-si, Geonggi-do 17104, Korea
2
Department of Medical Engineering, Graduate School, Kyung Hee University, Seoul 02447, Korea
3
R&D Institute, Biosolution Inc., Seoul 18111, Korea
4
Department of Biomedical Engineering, School of Medicine, Kyung Hee University, Seoul 02447, Korea
5
Department of Genetic Engineering, College of Life Science, Kyung Hee University, Seochon-dong, Kiheung-go, Yongin-si, Geonggi-do 17104, Korea
6
Impedance Imaging Research Center, Kyung Hee University, Seoul 02447, Korea
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(1), 325; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010325
Received: 11 December 2020 / Revised: 24 December 2020 / Accepted: 27 December 2020 / Published: 30 December 2020
(This article belongs to the Special Issue Novel Extracellular and Intracellular Signalling Mechanisms in Bone)
Near the bone remodeling compartments (BRC), extracellular calcium concentration (Ca2+o) is locally elevated and bone marrow stromal cells (BMSCs) close to the BRC can be exposed to high calcium concentration. The calcium-sensing receptor (CaSR) is known to play a key role in maintaining extracellular calcium homeostasis by sensing fluctuations in the levels of extracellular calcium (Ca2+o). When human BMSCs (hBMSCs) were exposed to various calcium concentrations (1.8, 3, 5, 10, 30 mM), moderate-high extracellular calcium concentrations (3–5 mM) stimulated proliferation, while a high calcium concentration (30 mM) inhibited the proliferation. Exposure to various calcium concentrations did not induce significant differences in the apoptotic cell fraction. Evaluation of multi-lineage differentiation potential showed no significant difference among various calcium concentration groups, except for the high calcium concentration (30 mM) treated group, which resulted in increased calcification after in vitro osteogenic differentiation. Treatment of NPS2143, a CaSR inhibitor, abolished the stimulatory effect on hBMSCs proliferation and migration indicating that CaSR is involved. These results suggest that the calcium concentration gradient near the BRC may play an important role in bone remodeling by acting as an osteoblast–osteoclast coupling mechanism through CaSR. View Full-Text
Keywords: bone remodeling; calcium-sensing receptor; proliferation; osteoblast-osteoclast coupling bone remodeling; calcium-sensing receptor; proliferation; osteoblast-osteoclast coupling
Show Figures

Figure 1

MDPI and ACS Style

Cho, H.; Lee, J.; Jang, S.; Lee, J.; Oh, T.I.; Son, Y.; Lee, E. CaSR-Mediated hBMSCs Activity Modulation: Additional Coupling Mechanism in Bone Remodeling Compartment. Int. J. Mol. Sci. 2021, 22, 325. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010325

AMA Style

Cho H, Lee J, Jang S, Lee J, Oh TI, Son Y, Lee E. CaSR-Mediated hBMSCs Activity Modulation: Additional Coupling Mechanism in Bone Remodeling Compartment. International Journal of Molecular Sciences. 2021; 22(1):325. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010325

Chicago/Turabian Style

Cho, Hyunji, Jisoo Lee, Seoyoung Jang, Jungsun Lee, Tong I. Oh, Youngsook Son, and EunAh Lee. 2021. "CaSR-Mediated hBMSCs Activity Modulation: Additional Coupling Mechanism in Bone Remodeling Compartment" International Journal of Molecular Sciences 22, no. 1: 325. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010325

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop