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Article

Distinctive Properties of Endothelial Cells from Tumor and Normal Tissue in Human Breast Cancer

1
Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine, PL-04-141 Warsaw, Poland
2
Postgraduate School of Molecular Medicine, Medical University of Warsaw, PL-02-091 Warsaw, Poland
3
Laboratory of Human Molecular Genetics, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Poznan, 61-614 Poznan, Poland
4
Center for Molecular Biophysics UPR 4301 CNRS, 45071 Orleans, France
*
Author to whom correspondence should be addressed.
Academic Editors: Ignacio Benedicto and Maria Luisa Balestrieri
Int. J. Mol. Sci. 2021, 22(16), 8862; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22168862
Received: 7 July 2021 / Revised: 5 August 2021 / Accepted: 12 August 2021 / Published: 17 August 2021
Tumor microenvironments shape aggressiveness and are largely maintained by the conditions of angiogenesis formation. Thus, endothelial cells’ (ECs) biological reactions are crucial to understand and control the design of efficient therapies. In this work, we used models of ECs to represent a breast cancer tumor site as well as the same, healthy tissue. Cells characterization was performed at the transcriptome and protein expression levels, and the cells functional biological responses (angiogenesis and permeability) were assessed. We showed that the expression of proteins specific to ECs (ACE+, VWF+), their differentiation (CD31+, CD 133+, CD105+, CD34-), their adhesion properties (ICAM-1+, VCAM-1+, CD62-L+), and their barrier formation (ZO-1+) were all downregulated in tumor-derived ECs. NGS-based differential transcriptome analysis confirmed CD31-lowered expression and pointed to the increase of Ephrin-B2 and SNCAIP, indicative of dedifferentiation. Functional assays confirmed these differences; angiogenesis was impaired while permeability increased in tumor-derived ECs, as further validated by the distinctly enhanced VEGF production in response to hypoxia, reflecting the tumor conditions. This work showed that endothelial cells differed highly significantly, both phenotypically and functionally, in the tumor site as compared to the normal corresponding tissue, thus influencing the tumor microenvironment. View Full-Text
Keywords: microenvironment; angiogenesis; endothelial cells; breast cancer; organospecificity; vascular dysfunction microenvironment; angiogenesis; endothelial cells; breast cancer; organospecificity; vascular dysfunction
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MDPI and ACS Style

Wilkus, K.; Brodaczewska, K.; Kajdasz, A.; Kieda, C. Distinctive Properties of Endothelial Cells from Tumor and Normal Tissue in Human Breast Cancer. Int. J. Mol. Sci. 2021, 22, 8862. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22168862

AMA Style

Wilkus K, Brodaczewska K, Kajdasz A, Kieda C. Distinctive Properties of Endothelial Cells from Tumor and Normal Tissue in Human Breast Cancer. International Journal of Molecular Sciences. 2021; 22(16):8862. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22168862

Chicago/Turabian Style

Wilkus, Kinga, Klaudia Brodaczewska, Arkadiusz Kajdasz, and Claudine Kieda. 2021. "Distinctive Properties of Endothelial Cells from Tumor and Normal Tissue in Human Breast Cancer" International Journal of Molecular Sciences 22, no. 16: 8862. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22168862

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