Silencing a Simple Extracellular Leucine-Rich Repeat Gene OsI-BAK1 Enhances the Resistance of Rice to Brown Planthopper Nilaparvata lugens
Abstract
:1. Introduction
2. Results
2.1. Isolation and Characterization of OsI-BAK1
2.2. Herbivore Infestation, Wounding and JA and Abscisic Acid Treatment Enhance Transcript Levels of OsI-BAK1
2.3. Silencing OsI-BAK1 in Rice
2.4. OsI-BAK1 Negatively Regulates the Transcript Level of Defense-Related WRKYs
2.5. Silencing OsI-BAK1 Reduces the Accumulation of BPH-Induced Ethylene
2.6. Silencing OsI-BAK1 Enhances the Resistance of Rice to BPH
3. Discussion
4. Materials and Methods
4.1. Plant Growth and Insects
4.2. Isolation of OsI-BAK1 cDNA
4.3. Structure and Phylogenetic Analysis of OsI-BAK1
4.4. Subcellular Localization Assay
4.5. Plant Treatment
4.6. RNA Extraction and Real Time-qPCR
4.7. Generation and Characterization of Transgenic Plants
4.8. Measurement of Plant Growth Parameters
4.9. BPH Bioassay
4.10. Detection of MPK Activation
4.11. SA, JA, JA-Ile, ABA and H2O2 Analysis
4.12. Ethylene Analysis
4.13. Data Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ye, M.; Kuai, P.; Chen, S.; Lin, N.; Ye, M.; Hu, L.; Lou, Y. Silencing a Simple Extracellular Leucine-Rich Repeat Gene OsI-BAK1 Enhances the Resistance of Rice to Brown Planthopper Nilaparvata lugens. Int. J. Mol. Sci. 2021, 22, 12182. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212182
Ye M, Kuai P, Chen S, Lin N, Ye M, Hu L, Lou Y. Silencing a Simple Extracellular Leucine-Rich Repeat Gene OsI-BAK1 Enhances the Resistance of Rice to Brown Planthopper Nilaparvata lugens. International Journal of Molecular Sciences. 2021; 22(22):12182. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212182
Chicago/Turabian StyleYe, Miaofen, Peng Kuai, Shuting Chen, Na Lin, Meng Ye, Lingfei Hu, and Yonggen Lou. 2021. "Silencing a Simple Extracellular Leucine-Rich Repeat Gene OsI-BAK1 Enhances the Resistance of Rice to Brown Planthopper Nilaparvata lugens" International Journal of Molecular Sciences 22, no. 22: 12182. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212182