Nanoparticle-Mediated Delivery Systems in Photodynamic Therapy of Colorectal Cancer
Abstract
:1. Introduction
2. PDT in CRC Therapeutics
3. Principle of PDT
4. Cell Death Mechanism Associated with PDT
5. Photosensitizers Used in CRC
5.1. First-Generation PSs
5.2. Second-Generation PSs
5.3. Third-Generation PSs
PS | Generation | Cell Type | Remarks | Ref. |
---|---|---|---|---|
Sinoporphyrin sodium and photofrin | 2nd, 1st | HCT-8 and HCT-116 | The effects of sinoporphyrin sodium-PDT and photofrin-PDT resulted in significant antitumour efficacy | [54] |
Tetraaryl brominated porphyrin (TBr4) and with the diaryl (BBr2) derivative. | 2nd | Colorectal adenocarcinoma cells, HT29 | Significant reduction in cell growth and necrotic cell death within in vitro and in vivo studies | [55] |
Gallium (III) phthalocyanine chloride (GaPcCl) | 2nd | Caco-2 | GaPcCl with PDT led to 60% to 80% cell viability cytotoxic and apoptotic cell death. | [56] |
Tetra 4-(3-(piperidinium-1-ylmethyl) phenoxy substituted zinc (II) phthalocyanine (Zn6a) | 2nd | colorectal carcinoma (HCT-116) | High phototoxicity on HCT-116 cells | [57] |
Selenium tetrasubstituted zinc (II) phthalocyanines | 2nd | Murine colon carcinoma CT26 | Significant increment in ROS level and efficient antitumour effect. | [58] |
Hypericin (HY) | 2nd | SW480 and SW620 | HY mediated PDT demonstrated cytotoxic effect and inhibition of tumour cell proliferation in a dose-dependent manner. | [59] |
Chlorin e6 (Ce6) | 2nd | SW620 | Ce6 mediated PDT significantly reduced the healing and migration rate of colon cells. | [60] |
5-aminolevulinic acid | 2nd | SW480 and SW620 | PDT with 5-ALA improved anticancer effects and inhibited of the secretion of cytokines (IL-10) | [61] |
Ce6 | 2nd | SW480 | Decreased cell survival rate in a dose-dependent manner and significant inhibitory effect on F-actin microfilament and cytoskeleton. | [62] |
5-aminolevulinic acid | 2nd | Caco-2 | Cell viability inhibition~62.4%, and improved antitumour efficacy | [63] |
5,10,15,20-Tetra(quinolin-2-yl) porphyrin (2TQP) | 2nd | HT29 colorectal adenocarcinoma | 2-TQP displayed effective phototoxic effects with no dark toxicity on cells | [64] |
Hypericin (HYP) | 2nd | HCT116 and SW620 | Cell proliferation inhibited, and efficient ROS generated by HYP-PDT treatment. Apoptosis was induced | [65] |
Sinoporphyrin sodium (DVDMS) | 2nd | CX-1 | DVDMS-PDT triggered apoptosis. Inhibitory effect in a dose and time dependent manner | [66] |
6. Current Limitations of CRC PDT
7. Nanotechnology as a Favourable Strategy in PDT for CRC Therapy
7.1. NPs-Mediated PS Delivery in CRC PDT
7.1.1. Passive Targeting Strategy
7.1.2. Active Targeting Strategy
8. PDT Combined with Other Therapies in CRC Treatment
9. Application of 3D Tumour Models in PDT CRC Treatment
10. Clinical Application of PDT in CRC Treatment
11. Conclusions and Future Perspectives
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
Abbreviations
2D | Two-dimensional models |
3D | Three-dimensional models |
AuNPs | Gold nanoparticles |
CRC | Colorectal cancer |
ECM | Extracellular matrix |
e6 | Chlorin e6 |
EGFR | Epidermal growth factor receptors |
FOBT | Faecal occult blood test |
HpD | Hematoporphyrin derivative |
mAb | Monoclonal antibodies |
MCTS | Multicellular tumour spheroids |
NIR | Near infrared |
NPs | Nanoparticles |
PDT | Photodynamic therapy |
PSs | Photosensitizers |
PTT | Photothermal therapy |
ROS | Reactive oxygen species |
TNM | Tumour, nodes, metastasis |
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In Vivo CRC PDT Studies Reported on Passive Nanoparticle-Based Photosensitizers | ||||
---|---|---|---|---|
Nanosystem | PS | Key Findings | Cell Type | Ref. |
chitosan nanoparticles (CS NPs) | Encaspulated 5-Aminolevulinic acid 5-ALA and photothermal (IR780) | Superior photodynamic cytotoxicity effects, higher tumour accumulation | mouse colon tumours CT-26 cells | [87] |
lipid nanoparticles | HPPH | Effective accumulated in colon tumours and enhanced anticancer activity | Murine CT-26 colon carcinoma and HT29 tumour bearing mice | [88] |
PheoA-ss-GC nanoparticles (PheoA-ss-CNPs), | pheophorbidea (PheoA) | Increased selective accumulation and significant reduction in tumour growth | HT-29 tumour-bearing mice | [89] |
Functionalized polyacrylamide (AFPAA) | 2-[1-hexyloxyethyl]-2-devinyl pyropheophorbide-a (HPPH) | Improved localisation and the tumour response to the treatment was approximately 40%. | BALB/c mice bearing Colon26 tumours | [90] |
Chlorin-core star-shaped block copolymer (CSBC) | The combinative effects of chemotherapy and PDT -SN-38/CBSC demonstrated significant anticancer efficacy. | HT-29 xenograft model. | [91] |
In Vitro CRC PDT Studies Reported on Active Nanoparticle-Based Photosensitizers | |||||
---|---|---|---|---|---|
Nanosystem | Ligand/Moieties | PS | Key Findings | Cell Type | Ref. |
EGFR-hydrogel | EGFR antibody | chlorin e6 (Ce6) | Excellent synergistic anticancer effect with increased protein expression levels. | HT-29 (Human colon cancer cell lines) | [95] |
Liposomes encapsulated Ce6 and phosphoinositide 3-kinase gamma (PI3Kγ) inhibitor IPI-549 | IPI-549 | chlorin e6 | Efficient tumour targeting, and anticancer activity | CT26 cells | [96] |
Mannose-conjugated chlorin (M-chlorin) | Mannose- | M-chlorin | Higher tumour selectivity, increased cytotoxicity, and significantly suppressed tumour growth | HT29, HCT116, CT26 cells | [97] |
VPA moiety-platinum diimine complexes | VPA moiety | Platinum diimine complexes | Minimal dark toxicity and improved cytotoxic effect on cancer cells | SW480 human colon cancer cell line | [98] |
TPP-conjugated polymer-lipid hybrid nanoparticles | Triphenylphosphonium (TPP) | ZCNP/IR780 | Enhanced specific mitochondria-targeting and enhanced anticancer effect. | Human colon carcinoma (HT-29) and HT-29 cell-bearing xenograft | [99] |
In vivo CRC PDT studies reported on active nanoparticle-based photosensitizers | |||||
Nanosystem | PS | Key Findings | Cell Type | Ref. | |
Liposome encapsulated photosensitizer chlorin e6 (Ce6) and phosphoinositide 3-kinase gamma (PI3Kγ) inhibitor IPI-549 | IPI-549 | Ce6 | The nanoformulations improved PDT therapeutic effect | CT26 cells | [96] |
Verteporfin-loaded D-α-tocopheryl polyethylene glycol succinate (TPGS) nanoparticles modified with tLyp-1 tumour homing and peptide tLyp-1 decoration (t-NP) | tLyp-1 decoration (t-NP) peptide | Verteporfin (VP) | Higher tumour selectivity of PS, inhibition of tumour growth and enhanced in vivo photodynamic effects. | HCT15 colon cells | [100] |
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Winifred Nompumelelo Simelane, N.; Abrahamse, H. Nanoparticle-Mediated Delivery Systems in Photodynamic Therapy of Colorectal Cancer. Int. J. Mol. Sci. 2021, 22, 12405. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212405
Winifred Nompumelelo Simelane N, Abrahamse H. Nanoparticle-Mediated Delivery Systems in Photodynamic Therapy of Colorectal Cancer. International Journal of Molecular Sciences. 2021; 22(22):12405. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212405
Chicago/Turabian StyleWinifred Nompumelelo Simelane, Nokuphila, and Heidi Abrahamse. 2021. "Nanoparticle-Mediated Delivery Systems in Photodynamic Therapy of Colorectal Cancer" International Journal of Molecular Sciences 22, no. 22: 12405. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212405