Effects of the Combined Treatment with a G-Quadruplex-Stabilizing Ligand and Photon Beams on Glioblastoma Stem-like Cells: A Magnetic Resonance Study
Abstract
:1. Introduction
2. Results
3. Discussion
- (i)
- Line #83 was only slightly affected by irradiation, as previously observed in Palma et al. [12]. Ligand #190 induced a growth arrest; however, further cell irradiation did not induce any additional effect;
- (ii)
- Line #1 cells showed a slowdown in growth after the combined treatments with radiation and ligand #190. Cell growth was mainly slowed down by the action of the ligand, while the combined treatment gave only a moderate synergistic effect;
- (iii)
- MRS experiments on the two cell lines showed significant effects on several metabolites, mainly on lipid signals.
3.1. Lipid Metabolism in Gliomas
3.2. Other Metabolic Changes in Glioma Cells
3.3. Glutatione (GSH) Metabolism in Gliomas
3.4. Myo-I Metabolism in Gliomas
3.5. Metabolism of NAA in Gliomas
4. Materials and Methods
4.1. Ligand #190 Synthesis
4.2. GSC Isolation and Cell Culture
4.3. Ligand #190 and Irradiation Treatment
4.4. H MRS Cell Sample Preparation
4.5. H MRS Measurements
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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p-Value | ||||||
Line #1 | A | Myo-I | GSH | NAA | Gln | Glu |
CON-TR | 0.0002 | 0.0002 | 0.9608 | 0.0007 | 0.0134 | 0.0032 |
CON-TR+IR | 0.0039 | 0.0010 | 0.0122 | 0.0001 | 0.0002 | 0.0071 |
CON-IR | 0.0010 | 0.0130 | 0.0008 | 0.0030 | 0.0002 | 0.3438 |
TR-TR+IR | 0.2873 | 0.7910 | 0.3530 | 0.0808 | 0.1213 | 0.0706 |
TR-IR | 0.4845 | 0.6515 | 0.0540 | 0.9201 | 0.0677 | 0.1829 |
IR-TR+IR | 0.6508 | 0.4907 | 0.0076 | 0.0004 | 0.6543 | 0.0599 |
p-Value | ||||||
---|---|---|---|---|---|---|
Line #83 | A | Myo-I | GSH | NAA | Gln | Glu |
CON-TR | 0.8213 | 0.0005 | 0.7732 | 0.3474 | 0.0184 | 0.8406 |
CON-TR+IR | 0.0001 | 0.0050 | 0.0815 | 0.0797 | 0.5177 | 0.0337 |
CON-IR | 0.0506 | 0.0415 | 0.1096 | 0.6933 | 0.0258 | 0.0689 |
TR-TR+IR | 0.0056 | 0.5769 | 0.1536 | 0.2222 | 0.1070 | 0.0743 |
TR-IR | 0.2069 | 0.8341 | 0.1990 | 0.8991 | 0.0001 | 0.0766 |
IR-TR+IR | 0.0795 | 0.2535 | 0.6462 | 0.4463 | 0.0001 | 0.0006 |
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Palma, A.; Grande, S.; Luciani, A.M.; Ricci-Vitiani, L.; Buccarelli, M.; Pallini, R.; Triveri, A.; Pirota, V.; Doria, F.; D’Alessandris, Q.G.; et al. Effects of the Combined Treatment with a G-Quadruplex-Stabilizing Ligand and Photon Beams on Glioblastoma Stem-like Cells: A Magnetic Resonance Study. Int. J. Mol. Sci. 2021, 22, 12709. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222312709
Palma A, Grande S, Luciani AM, Ricci-Vitiani L, Buccarelli M, Pallini R, Triveri A, Pirota V, Doria F, D’Alessandris QG, et al. Effects of the Combined Treatment with a G-Quadruplex-Stabilizing Ligand and Photon Beams on Glioblastoma Stem-like Cells: A Magnetic Resonance Study. International Journal of Molecular Sciences. 2021; 22(23):12709. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222312709
Chicago/Turabian StylePalma, Alessandra, Sveva Grande, Anna Maria Luciani, Lucia Ricci-Vitiani, Mariachiara Buccarelli, Roberto Pallini, Alice Triveri, Valentina Pirota, Filippo Doria, Quintino Giorgio D’Alessandris, and et al. 2021. "Effects of the Combined Treatment with a G-Quadruplex-Stabilizing Ligand and Photon Beams on Glioblastoma Stem-like Cells: A Magnetic Resonance Study" International Journal of Molecular Sciences 22, no. 23: 12709. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222312709