Enhancing the Expression of the OsF3H Gene in Oryza sativa Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance
Abstract
:1. Introduction
2. Results and Discussion
2.1. Gene Ontology (GO) Enrichment and Pathway Analysis
2.2. DEGs Involved in the JA, BR, SA, and ET Signaling Pathways
2.3. Expression of Pathogenesis-Related Expression (PR) and Nonexpressor (NPR) Genes
2.4. WRKY Transcription Factor and MAPK Genes Expression during WBPH Stress
2.5. RNA-Seq Data Validation by RT-qPCR
3. Materials and Methods
3.1. Plant Material
3.2. RNA Extraction, Library Construction, and Sequencing
3.3. Bioinformatics Analyses
3.4. Reverse Transcription Quantitative PCR (RT-qPCR)
3.5. Quantification of Endogenous SA and JA
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jan, R.; Asaf, S.; Lubna; Asif, S.; Kim, E.-G.; Jang, Y.-H.; Kim, N.; Al-Harrasi, A.; Lee, G.-S.; Kim, K.-M. Enhancing the Expression of the OsF3H Gene in Oryza sativa Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance. Int. J. Mol. Sci. 2022, 23, 15308. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232315308
Jan R, Asaf S, Lubna, Asif S, Kim E-G, Jang Y-H, Kim N, Al-Harrasi A, Lee G-S, Kim K-M. Enhancing the Expression of the OsF3H Gene in Oryza sativa Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance. International Journal of Molecular Sciences. 2022; 23(23):15308. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232315308
Chicago/Turabian StyleJan, Rahmatullah, Sajjad Asaf, Lubna, Saleem Asif, Eun-Gyeong Kim, Yoon-Hee Jang, Nari Kim, Ahmed Al-Harrasi, Gang-Seob Lee, and Kyung-Min Kim. 2022. "Enhancing the Expression of the OsF3H Gene in Oryza sativa Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance" International Journal of Molecular Sciences 23, no. 23: 15308. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232315308