Lipid Profiles of Urinary Extracellular Vesicles Released during the Inactive and Active Phases of Aged Male Mice with Spontaneous Hypertension
Abstract
:1. Introduction
2. Results
2.1. Amiloride Reduces Systolic Blood Pressure in the Inactive and Active Cycles of Aged Male Mice with Spontaneous Hypertension
2.2. Urinary Electrolyte Concentration during the Inactive and Active Phases of Aged Male Mice
2.3. Concentration of EVs Isolated from the Inactive and Active Phases of Aged Male Mice with Spontaneous Hypertension
2.4. Lipidomic Profiles of uEVs from Aged Male Mice with Spontaneous Hypertension
2.5. uEVs from Aged Mice Augment ENaC Activity in Recipient Mouse mpkCCD Cells
2.6. ENaC’s Association with Lipid Rafts from mpkCCD Cells Is Augmented after Treating the Cells with EVs Isolated from the Urine Collected during the Active Phase of Aged Mice
3. Discussion
4. Materials and Methods
4.1. Animal Diet and Metabolic Cage Studies
4.2. Benzyl Amiloride Treatment
4.3. Blood Pressure Measurements
4.4. Electrolyte Measurements
4.5. Isolation and Characterization of Urinary Extracellular Vesicles
4.6. Western Blotting of Lysed uEVs
4.7. Extraction of Lipids
4.8. Liquid Chromatography with Tandem Mass Spectrometry
4.9. Quantification of Lipids
4.10. mpkCCD Cell Culture
4.11. Amiloride-Sensitive Transepithelial Current Measurements
4.12. Sucrose Density Gradient Assays
4.13. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lopez, J.P.; Nouri, M.-Z.; Ebrahim, A.; Chacko, K.M.; Schramm, W.C.; Gholam, M.F.; Ozrazgat-Baslanti, T.; Denslow, N.D.; Alli, A.A. Lipid Profiles of Urinary Extracellular Vesicles Released during the Inactive and Active Phases of Aged Male Mice with Spontaneous Hypertension. Int. J. Mol. Sci. 2022, 23, 15397. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232315397
Lopez JP, Nouri M-Z, Ebrahim A, Chacko KM, Schramm WC, Gholam MF, Ozrazgat-Baslanti T, Denslow ND, Alli AA. Lipid Profiles of Urinary Extracellular Vesicles Released during the Inactive and Active Phases of Aged Male Mice with Spontaneous Hypertension. International Journal of Molecular Sciences. 2022; 23(23):15397. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232315397
Chicago/Turabian StyleLopez, Juliana Pena, Mohammad-Zaman Nouri, Areej Ebrahim, Kevin M. Chacko, Whitney C. Schramm, Mohammed F. Gholam, Tezcan Ozrazgat-Baslanti, Nancy D. Denslow, and Abdel A. Alli. 2022. "Lipid Profiles of Urinary Extracellular Vesicles Released during the Inactive and Active Phases of Aged Male Mice with Spontaneous Hypertension" International Journal of Molecular Sciences 23, no. 23: 15397. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232315397