Processing of Distillery Stillage to Recover Phenolic Compounds with Ultrasound-Assisted and Microwave-Assisted Extractions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Preparation
2.2. Extraction Procedure
2.3. Determination of Total Polyphenol Content (TPC) and Total Flavonoid Content (TFC)
2.4. Determination of Phenolic Acid Content
2.5. Antioxidant Activity
2.5.1. ABTS Radical Scavenging Assay
2.5.2. DPPH Radical Scavenging Assay
2.5.3. FRAP Assay
2.6. Calculations and Statistical Analysis
3. Results and Discussion
3.1. Polyphenol Extraction Assisted by Microwaves and Ultrasounds
3.1.1. Effect of Extraction Time on Polyphenol Recovery
3.1.2. Effect of Solid-to-Solvent Ratio on Polyphenol Recovery
3.1.3. Effect of Solvent Concentration on Polyphenol Recovery
3.1.4. pH and ST of the Extracts
3.2. Phenolic Acids
3.2.1. Content of Phenolic Acids in the Extracts
3.2.2. Kinetics of UAE and MAE
3.3. Antioxidant Activity
3.4. Should UAE or MAE Be Chosen for Polyphenol Recovery?
4. Conclusions and Future Perspectives
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Extraction | TFC (mg QUE/g DM) | Extraction | TFC (mg QUE/g DM) | |
---|---|---|---|---|
Solid-to-solvent ratio of 1:15 (w:v) | UAE3 A40 | 0.40 ± 0.10 | MAE1 A40 | 0.36 ± 0.44 |
UAE3 A60 | 0.50 ± 0.18 | MAE1 A60 | 0.44 ± 0.36 | |
UAE3 A80 | 0.43 ± 0.09 | MAE1 A80 | 0.40 ± 0.38 | |
UAE3 A100 | 0.26 ± 0.16 | MAE1 A100 | 0.23 ± 0.27 | |
UAE5 A40 | 0.49 ± 0.24 | MAE3 A40 | 0.45 ± 0.31 | |
UAE5 A60 | 0.60 ± 0.42 | MAE3 A60 | 0.53 ± 0.56 | |
UAE5 A80 | 0.50 ± 0.36 | MAE3 A80 | 0.44 ± 0.41 | |
UAE5 A100 | 0.31 ± 0.38 | MAE3 A100 | 0.27 ± 0.22 | |
UAE10 A40 | 0.58 ± 0.39 | MAE5 A40 | 0.52 ± 0.58 | |
UAE10 A60 | 0.72 ± 0.58 | MAE5 A60 | 0.61 ± 0.17 | |
UAE10 A80 | 0.59 ± 0.42 | MAE5 A80 | 0.54 ± 0.55 | |
UAE10 A100 | 0.38 ± 0.37 | MAE5 A100 | 0.35 ± 0.43 | |
UAE15 A40 | 0.52 ± 0.26 | MAE8 A40 | 0.47 ± 0.33 | |
UAE15 A60 | 0.64 ± 0.54 | MAE8 A60 | 0.57 ± 0.41 | |
UAE15 A80 | 0.53 ± 0.55 | MAE8 A80 | 0.47 ± 0.22 | |
UAE15 A100 | 0.35 ± 0.49 | MAE8 A100 | 0.32 ± 0.18 | |
UAE20 A40 | 0.43 ± 0.57 | MAE10 A40 | 0.35 ± 0.19 | |
UAE20 A60 | 0.57 ± 0.61 | MAE10 A60 | 0.52 ± 0.59 | |
UAE20 A80 | 0.46 ± 0.55 | MAE10 A80 | 0.41 ± 0.27 | |
UAE20 A100 | 0.28 ± 0.42 | MAE10 A100 | 0.25 ± 0.12 | |
Solid-to-solvent ratio of 1:30 (w:v) | UAE3 A40 | 0.35 ± 0.21 | MAE1 A40 | 0.31 ± 0.16 |
UAE3 A60 | 0.49 ± 0.26 | MAE1 A60 | 0.43 ± 0.38 | |
UAE3 A80 | 0.38 ± 0.32 | MAE1 A80 | 0.36 ± 0.41 | |
UAE3 A100 | 0.24 ± 0.18 | MAE1 A100 | 0.21 ± 0.09 | |
UAE5 A40 | 0.42 ± 0.23 | MAE3 A40 | 0.38 ± 0.27 | |
UAE5 A60 | 0.58 ± 0.44 | MAE3 A60 | 0.50 ± 0.42 | |
UAE5 A80 | 0.45 ± 0.37 | MAE3 A80 | 0.40 ± 0.39 | |
UAE5 A100 | 0.28 ± 0.19 | MAE3 A100 | 0.23 ± 0.27 | |
UAE10 A40 | 0.50 ± 0.43 | MAE5 A40 | 0.42 ± 0.34 | |
UAE10 A60 | 0.69 ± 0.54 | MAE5 A60 | 0.64 ± 0.59 | |
UAE10 A80 | 0.53 ± 0.51 | MAE5 A80 | 0.50 ± 0.39 | |
UAE10 A100 | 0.35 ± 0.38 | MAE5 A100 | 0.32 ± 0.26 | |
UAE15 A40 | 0.45 ± 0.52 | MAE8 A40 | 0.36 ± 0.14 | |
UAE15 A60 | 0.62 ± 0.59 | MAE8 A60 | 0.55 ± 0.48 | |
UAE15 A80 | 0.48 ± 0.58 | MAE8 A80 | 0.42 ± 0.44 | |
UAE15 A100 | 0.32 ± 0.48 | MAE8 A100 | 0.29 ± 0.22 | |
UAE20 A40 | 0.37 ± 0.36 | MAE10 A40 | 0.30 ± 0.19 | |
UAE20 A60 | 0.56 ± 0.25 | MAE10 A60 | 0.49 ± 0.34 | |
UAE20 A80 | 0.41 ± 0.58 | MAE10 A80 | 0.39 ± 0.26 | |
UAE20 A100 | 0.26 ± 0.42 | MAE10 A100 | 0.23 ± 0.29 |
Compound | Vm (mL/mol) | δD | δP | δH | δT |
---|---|---|---|---|---|
water | 18.06 | 15.5 | 16.0 | 42.3 | 47.8 |
acetone | 74.08 | 15.5 | 10.4 | 7.0 | 19.9 |
ferulic acid | 136.75 | 22.7 | 5.7 | 15.6 | 28.1 |
vanillic acid | 119.25 | 22.6 | 6.5 | 16.6 | 28.8 |
p-coumaric acid | 118.10 | 20.4 | 5.6 | 16.0 | 26.5 |
p-OH benzoic acid | 94.60 | 22.9 | 7.0 | 17.8 | 29.8 |
syringic acid | 151.28 | 21.2 | 5.8 | 15.4 | 26.8 |
sinapic acid | 167.32 | 21.6 | 5.2 | 14.7 | 26.6 |
Type of Extraction | Extraction Rate (µg/(g DM·Min)) | ||||||
---|---|---|---|---|---|---|---|
p-OH Benzoic | Vanillic | Syringic | p-Coumaric | Ferulic | Sinapic | Total Acids | |
UAE A40 1:15 | 0.047 | 0.085 | 0.038 | 0.178 | 0.348 | 0.062 | 0.758 |
UAE A60 1:15 | 0.070 | 0.130 | 0.058 | 0.249 | 0.539 | 0.095 | 1.142 |
UAE A80 1:15 | 0.054 | 0.098 | 0.045 | 0.206 | 0.403 | 0.071 | 0.878 |
UAE A100 1:15 | 0.034 | 0.062 | 0.028 | 0.131 | 0.255 | 0.045 | 0.557 |
UAE A40 1:30 | 0.038 | 0.069 | 0.032 | 0.145 | 0.284 | 0.051 | 0.620 |
UAE A60 1:30 | 0.065 | 0.103 | 0.055 | 0.191 | 0.458 | 0.089 | 0.958 |
UAE A80 1:30 | 0.049 | 0.079 | 0.040 | 0.166 | 0.306 | 0.064 | 0.703 |
UAE A100 1:30 | 0.026 | 0.049 | 0.023 | 0.089 | 0.188 | 0.036 | 0.412 |
MAE A40 1:15 | 0.151 | 0.272 | 0.125 | 0.570 | 1.118 | 0.199 | 2.435 |
MAE A60 1:15 | 0.250 | 0.453 | 0.206 | 0.850 | 1.846 | 0.328 | 3.929 |
MAE A80 1:15 | 0.180 | 0.330 | 0.150 | 0.687 | 1.342 | 0.238 | 3.417 |
MAE A100 1:15 | 0.099 | 0.180 | 0.081 | 0.375 | 0.732 | 0.129 | 1.594 |
MAE A40 1:30 | 0.116 | 0.211 | 0.095 | 0.442 | 0.860 | 0.153 | 1.875 |
MAE A60 1:30 | 0.306 | 0.400 | 0.214 | 0.715 | 1.728 | 0.339 | 3.647 |
MAE A80 1:30 | 0.179 | 0.294 | 0.152 | 0.619 | 1.138 | 0.238 | 2.618 |
MAE A100 1:30 | 0.079 | 0.154 | 0.071 | 0.275 | 0.581 | 0.111 | 1.272 |
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Mikucka, W.; Zielinska, M.; Bulkowska, K.; Witonska, I. Processing of Distillery Stillage to Recover Phenolic Compounds with Ultrasound-Assisted and Microwave-Assisted Extractions. Int. J. Environ. Res. Public Health 2022, 19, 2709. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph19052709
Mikucka W, Zielinska M, Bulkowska K, Witonska I. Processing of Distillery Stillage to Recover Phenolic Compounds with Ultrasound-Assisted and Microwave-Assisted Extractions. International Journal of Environmental Research and Public Health. 2022; 19(5):2709. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph19052709
Chicago/Turabian StyleMikucka, Wioleta, Magdalena Zielinska, Katarzyna Bulkowska, and Izabela Witonska. 2022. "Processing of Distillery Stillage to Recover Phenolic Compounds with Ultrasound-Assisted and Microwave-Assisted Extractions" International Journal of Environmental Research and Public Health 19, no. 5: 2709. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph19052709