Microbial Profile of the Leachate from Mexico City’s Bordo Poniente Composting Plant: An Inoculum to Digest Organic Waste
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling and Preparation of Substrate and Inoculum
2.2. Biochemical Methane Potential (BMP) Assay
2.3. Chemical Analysis
2.4. Generation and Composition of Biogas
2.5. Counting Mesophilic and Thermophilic Groups
2.6. Biochemical Tests
2.7. DNA Extraction and 16S rDNA Library Preparation
2.8. High Throughput Sequencing and Analysis
2.9. Bacterial and Archaeal Relative Abundance and Diversity
2.10. Predicted Metabolic Pathways of Bacteria and Archaea
2.11. Statistical Analysis
3. Results
3.1. Chemical Characteristics of the OFMSW, Inoculum, and Reactor Mixtures at the End of the 30-Day Period
3.2. Biochemical Methane Potential Assay
3.3. Counting Mesophilic and Thermophilic Groups
3.4. Bacterial Identification
3.5. Microbial Analysis
3.6. Diversity of Microbial Communities
3.7. Predicted Metabolic Pathways of Bacteria and Archaea
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameters | OFMSW | LCP | INCP |
---|---|---|---|
pH | 4.55 ± 0.05 | 6.89 ± 0.03 | 8.33 ± 0.01 |
ORP (mV) | 47.00 ± 1.73 | −284.00 ± 3.61 | −335.67 ± 3.79 |
EC (mS/cm) | 17.42 ± 0.92 | 33.35 ± 0.05 | 34.77 ± 0.26 |
TS (g/kg) | 288.57 ± 34.70 | 56.40 ± 2.56 | 47.70 ± 6.17 |
VS (g/kg) | 232.44 ± 28.14 | 29.75 ± 1.33 | 23.53 ± 3.30 |
VS/TS ratio (%) | 80.54 ± 0.14 | 52.76 ± 0.22 | 49.29 ± 1.26 |
Parameters | NC30 | PC30 | SB30 | p Value |
---|---|---|---|---|
pH | 8.51 ± 0.12 | 7.98 ± 0.14 | 7.75 ± 0.16 | 0.001 a |
ORP (mV) | −366.00 ± 11.53 | −379.00 ± 9.00 | −378.67 ± 3.06 | 0.245 b |
EC (mS/cm) | 32.17 ± 4.04 | 34.16 ± 3.77 | 40.80 ± 1.35 | 0.041 a |
TS (g/kg) | 45.68 ± 0.76 | 39.57 ± 0.75 | 43.73 ± 7.08 | 0.258 a |
VS (g/kg) | 13.90 ± 1.70 | 8.22 ± 0.44 | 19.96 ± 1.78 | 0.004 b |
VS/TS ratio | 30.41 ± 3.23 | 20.78 ± 0.73 | 46.01 ± 3.89 | 0.004 b |
Mesophilic (CFU/mL) | Thermophilic (MPN/mL) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
LCP | INCP | Treatments | Day 17 | Day 30 | LCP | INCP | Treatments | Day 17 | Day 30 | |
TAB | 6.4 × 105 | 5.9 × 105 | NC | 3.7 × 104 | 5.3 × 105 | 4.3 × 104 | 4.3 × 104 | NC | 9.5 × 104 | 2.9 × 103 |
PC | 3.5 × 104 | 3.2 × 105 | PC | 2.4 × 105 | 1.5 × 104 | |||||
SB | 3.7 × 104 | 2.8 × 105 | SB | 2.4 × 105 | 1.6 × 103 | |||||
CA | 4.6 × 106 | 8.9 × 104 | NC | 9.1 × 104 | 6.9 × 106 | 35 | 1.1 × 103 | NC | 3.5 × 103 | 3.5 × 103 |
PC | 4.9 × 107 | 1.5 × 105 | PC | 3.5 × 103 | 2.1 × 104 | |||||
SB | 6.1 × 106 | 8.2 × 104 | SB | 1.5 × 103 | 2.0 × 103 |
Sample | Taxa (%) | ||||
---|---|---|---|---|---|
LCP | g_Bacteroides (40.29) | f_Porphyromonadaceae (18.59) | g_Acidaminococcus (3.35) | f_Lachnospiraceae (2.48) | Others (35.30) |
INCP | o_MBA08 (47.07) | c_OPB54 (6.95) | o_ML615J-2 (5.59) | g_Acinetobacter (4.86) | Others (35.52) |
NC17 | g_Acinetobacter (36.25) | o_MBA08 (10.65) | g_S1 (4.91) | f_Porphyromonadaceae (3.90) | Others (44.28) |
NC30 | o_MBA08 (46.02) | g_S1 (5.31) | c_OPB54 (4.58) | f_Porphyromonadaceae (4.21) | Others (39.88) |
PC17 | f_Lachnospiraceae (42.57) | f_Halanaerobiaceae (11.03) | o_MBA08 (8.71) | f_Ruminococcaceae (6.07) | Others (31.63) |
PC30 | g_Bacteroides (26.82) | f_S24-7 (13.61) | f_Ruminococcaceae (10.21) | f_Lachnospiraceae (5.38) | Others (39.03) |
SB17 | f_Pseudomonadaceae (18.73) | o_MBA08 (13.60) | o_OPB54 (8.13) | c_OPB54 (6.19) | Others (53.35) |
SB30 | o_MBA08 (30.29) | o_OPB54 (8.66) | o_Bacteroidales (8.00) | f_Halanaerobiaceae (5.17) | Others (47.88) |
Sample | Taxa (%) | |||
---|---|---|---|---|
LCP | g_Methanosarcina (81.09) | g_Methanobrevibacter (16.10) | g_Methanoculleus (0.63) | Others (2.17) |
INCP | g_Methanoculleus (77.28) | g_Methanosarcina (15.42) | g_Methanomassiliicoccus (3.33) | Others (7.30) |
NC17 | g_vadinCA11 (54.08) | g_Methanoculleus (35.87) | g_Methanobrevibacter (3.47) | Others (6.58) |
NC30 | g_vadinCA11 (65.45) | g_Methanoculleus (25.60) | g_Methanosarcina (2.9681.09) | Others (6.00) |
PC17 | g_Methanoculleus (92.57) | g_vadinCA11 (4.19) | g_Methanosarcina (1.62) | Others (1.61) |
PC30 | g_Methanoculleus (75.07) | g_Methanothermobacter (11.35) | g_vadinCA11 (7.22) | Others (6.35) |
SB17 | g_Methanoculleus (91.67) | g_Methanosarcina (3.71) | g_vadinCA11 (2.44) | Others (2.17) |
SB30 | g_Methanoculleus (59.79) | g_vadinCA11 (29.24) | g_Methanosarcina (5.55) | Others (5.42) |
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Gállego Bravo, A.K.; Salcedo Serrano, D.A.; López Jiménez, G.; Nirmalkar, K.; Murugesan, S.; García-Mena, J.; Gutiérrez Castillo, M.E.; Tovar Gálvez, L.R. Microbial Profile of the Leachate from Mexico City’s Bordo Poniente Composting Plant: An Inoculum to Digest Organic Waste. Energies 2019, 12, 2343. https://0-doi-org.brum.beds.ac.uk/10.3390/en12122343
Gállego Bravo AK, Salcedo Serrano DA, López Jiménez G, Nirmalkar K, Murugesan S, García-Mena J, Gutiérrez Castillo ME, Tovar Gálvez LR. Microbial Profile of the Leachate from Mexico City’s Bordo Poniente Composting Plant: An Inoculum to Digest Organic Waste. Energies. 2019; 12(12):2343. https://0-doi-org.brum.beds.ac.uk/10.3390/en12122343
Chicago/Turabian StyleGállego Bravo, Aixa Kari, Daniel Alejandro Salcedo Serrano, Gloria López Jiménez, Khemlal Nirmalkar, Selvasankar Murugesan, Jaime García-Mena, María Eugenia Gutiérrez Castillo, and Luis Raúl Tovar Gálvez. 2019. "Microbial Profile of the Leachate from Mexico City’s Bordo Poniente Composting Plant: An Inoculum to Digest Organic Waste" Energies 12, no. 12: 2343. https://0-doi-org.brum.beds.ac.uk/10.3390/en12122343