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Article

Propagation, Inactivation, and Safety Testing of SARS-CoV-2

Center for Microbial Pathogenesis, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 303030, USA
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Author to whom correspondence should be addressed.
Those authors contributed equally to this manuscript.
Received: 13 May 2020 / Revised: 1 June 2020 / Accepted: 4 June 2020 / Published: 6 June 2020
(This article belongs to the Section SARS-CoV-2 and COVID-19)
In late 2019, a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, the capital of the Chinese province Hubei. Since then, SARS-CoV-2 has been responsible for a worldwide pandemic resulting in over 4 million infections and over 250,000 deaths. The pandemic has instigated widespread research related to SARS-CoV-2 and the disease that it causes, COVID-19. Research into this new virus will be facilitated by the availability of clearly described and effective procedures that enable the propagation and quantification of infectious virus. As work with the virus is recommended to be performed at biosafety level 3, validated methods to effectively inactivate the virus to enable the safe study of RNA, DNA, and protein from infected cells are also needed. Here, we report methods used to grow SARS-CoV-2 in multiple cell lines and to measure virus infectivity by plaque assay using either agarose or microcrystalline cellulose as an overlay as well as a SARS-CoV-2 specific focus forming assay. We also demonstrate effective inactivation by TRIzol, 10% neutral buffered formalin, beta propiolactone, and heat. View Full-Text
Keywords: SARS-CoV-2; coronavirus; virus; virology; plaque assay; inactivation SARS-CoV-2; coronavirus; virus; virology; plaque assay; inactivation
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MDPI and ACS Style

Jureka, A.S.; Silvas, J.A.; Basler, C.F. Propagation, Inactivation, and Safety Testing of SARS-CoV-2. Viruses 2020, 12, 622. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060622

AMA Style

Jureka AS, Silvas JA, Basler CF. Propagation, Inactivation, and Safety Testing of SARS-CoV-2. Viruses. 2020; 12(6):622. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060622

Chicago/Turabian Style

Jureka, Alexander S.; Silvas, Jesus A.; Basler, Christopher F. 2020. "Propagation, Inactivation, and Safety Testing of SARS-CoV-2" Viruses 12, no. 6: 622. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060622

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