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Article

Expression Level of DREB Gene of Local Corn Cultivars from Kisar Island-Maluku, Indonesia, Using Quantitative Real Time Polymerase Chain Reaction

by
Hermalina Sinay
1,* and
Estri Laras Arumingtyas
2
1
Biology Education Program, Faculty of Teaching and Education, Pattimura University, Ambon 97231, Indonesia
2
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, Indonesia
*
Author to whom correspondence should be addressed.
Int. J. Plant Biol. 2018, 9(1), 7005; https://0-doi-org.brum.beds.ac.uk/10.4081/pb.2018.7005
Submission received: 13 December 2016 / Revised: 12 January 2017 / Accepted: 12 January 2017 / Published: 24 May 2018

Abstract

The research objective was to determine the expression level of dehydration responsive element binding (DREB) gene of local corn cultivars from Kisar Island Maluku, Indonesia. The study was designed as randomized block design with single factor consist of six local corn cultivars obtained from farmers in Kisar Island and one reference varieties which has been released as a drought-tolerant varieties and obtained from Cereal Crops Research Institute Maros South Sulawesi. Isolation of total RNA from the second leaf after the flag leaf at the 65 days after planting were carried out according to the protocols of the R and ABlueTM Total RNA Extraction Kit, and was used as a template for cDNA synthesis. Amplification of cDNA from total RNA was carried out according to the protocol of One-Step Reverse Transcriptase PCR Premix Kit. Real Time-PCR was performed usingcDNA from reverse transcription following the procedures of Real MODTM Green Real-Time PCR Master Mix Kit. The real time-PCR data were analyzed using relative quantification method based on the critical point/cycle threshold. The highest DREB gene expression was showed by Deep Yellow local corn cultivar, and the lowest one was showed by Rubby Brown Cob cultivar. The DREB gene expression level of deep yellow local corn cultivar was even higher than Srikandi variety as a reference variety.
Keywords: expression level; DREB gene; local corn cultivars expression level; DREB gene; local corn cultivars

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MDPI and ACS Style

Sinay, H.; Arumingtyas, E.L. Expression Level of DREB Gene of Local Corn Cultivars from Kisar Island-Maluku, Indonesia, Using Quantitative Real Time Polymerase Chain Reaction. Int. J. Plant Biol. 2018, 9, 7005. https://0-doi-org.brum.beds.ac.uk/10.4081/pb.2018.7005

AMA Style

Sinay H, Arumingtyas EL. Expression Level of DREB Gene of Local Corn Cultivars from Kisar Island-Maluku, Indonesia, Using Quantitative Real Time Polymerase Chain Reaction. International Journal of Plant Biology. 2018; 9(1):7005. https://0-doi-org.brum.beds.ac.uk/10.4081/pb.2018.7005

Chicago/Turabian Style

Sinay, Hermalina, and Estri Laras Arumingtyas. 2018. "Expression Level of DREB Gene of Local Corn Cultivars from Kisar Island-Maluku, Indonesia, Using Quantitative Real Time Polymerase Chain Reaction" International Journal of Plant Biology 9, no. 1: 7005. https://0-doi-org.brum.beds.ac.uk/10.4081/pb.2018.7005

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