Sasa quelpaertensis Leaf Extract Ameliorates Dyslipidemia, Insulin Resistance, and Hepatic Lipid Accumulation in High-Fructose-Diet-Fed Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Preparation of SQE
2.2. Animals and Diet
2.3. Biochemical Parameter Assays
2.4. Western Blot Analysis
2.5. Histological Analysis
2.6. RNA Extraction, Library, and Sequencing
2.7. mRNA-Seq Data and Gene Expression Level
2.8. Statistical Analysis
3. Results
3.1. Effect of SQE on Changes in Body and Organ Weight
3.2. Effect of SQE on Serum Lipid Profiles
3.3. Effects of SQE on Glucose Homeostasis
3.4. Effects of SQE on Liver Function
3.5. Effects of SQE on Protein Expression in Liver Tissue
3.6. Effect of SQE on Liver Transcriptome Profiles
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Groups | HC | HF | SQE |
---|---|---|---|
Initial body weight (g) | 322.50 ± 17.77 | 322.87 ± 22.56 | 309.79 ± 12.87 |
Final body weight (g) | 699.63 ± 70.02 a | 703.13 ± 42.48 a | 638.75 ± 35.75 b |
Weight gain (g) | 377.12 ± 57.08 a | 380.25 ± 28.17 a | 329.00 ± 27.17 b |
Total food intake for 16 weeks (kcal/g) | 11,600.82 ± 79.22 a | 16,520.70 ± 148.46 b | 16,030.99 ± 113.54 b |
Total water intake for 16 weeks (mL) | 5197.25 ± 27.30 a | 6597.06 ± 37.49 b | 7476.96 ± 95.24 c |
Food efficiency ratio (%) | 9.31 ± 1.29 a | 8.43 ± 0.49 a | 7.28 ± 0.57 b |
Liver weight (g) | 14.93 ± 2.63 a | 20.15 ± 4.97 b | 15.11 ± 1.78 a |
Spleen weight (g) | 1.01 ± 0.19 a | 1.00 ± 0.14 a | 0.78 ± 0.09 b |
Kidney weight (g) | 1.69 ± 0.17 a | 2.08 ± 0.20 b | 1.87 ± 0.20 ab |
Testis weight (g) | 1.70 ± 0.11 | 1.82 ± 0.11 | 1.61 ± 0.38 |
Epididymal fat (g) | 0.43 ± 0.11 a | 0.57 ± 0.11 b | 0.42 ± 0.06 a |
Sample | Total Reads | GC Contents (%) | Q30 (%) | Processed Reads | Mapped Reads | Unmapped Reads |
---|---|---|---|---|---|---|
HF-1 | 63,992,692 | 49.00 | 95.58 | 63,992,692 | 62,592,420 (97.81%) | 1,400,273 (2.19%) |
HF-2 | 63,132,988 | 49.18 | 95.80 | 63,132,988 | 61,846,383 (97.96%) | 1,286,605 (2.04%) |
HF-3 | 75,518,180 | 49.50 | 95.59 | 75,518,180 | 73,953,123 (97.93%) | 1,565,057 (2.07%) |
SQE-1 | 83,369,682 | 49.13 | 95.71 | 83,396,682 | 81,437,777 (97.65%) | 1,958,905 (2.35%) |
SQE-2 | 64,484,030 | 49.11 | 95.83 | 64,484,030 | 63,011,809 (97.72%) | 1,472,221 (2.28%) |
SQE-3 | 87,240,986 | 49.30 | 95.74 | 87,240,986 | 85,412,156 (97.90%) | 1,828,830 (2.1%) |
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Park, J.Y.; Jang, M.G.; Oh, J.M.; Ko, H.C.; Hur, S.-P.; Kim, J.-W.; Baek, S.; Kim, S.-J. Sasa quelpaertensis Leaf Extract Ameliorates Dyslipidemia, Insulin Resistance, and Hepatic Lipid Accumulation in High-Fructose-Diet-Fed Rats. Nutrients 2020, 12, 3762. https://0-doi-org.brum.beds.ac.uk/10.3390/nu12123762
Park JY, Jang MG, Oh JM, Ko HC, Hur S-P, Kim J-W, Baek S, Kim S-J. Sasa quelpaertensis Leaf Extract Ameliorates Dyslipidemia, Insulin Resistance, and Hepatic Lipid Accumulation in High-Fructose-Diet-Fed Rats. Nutrients. 2020; 12(12):3762. https://0-doi-org.brum.beds.ac.uk/10.3390/nu12123762
Chicago/Turabian StylePark, Jeong Yong, Mi Gyeong Jang, Jung Min Oh, Hee Chul Ko, Sung-Pyo Hur, Jae-Won Kim, Songyee Baek, and Se-Jae Kim. 2020. "Sasa quelpaertensis Leaf Extract Ameliorates Dyslipidemia, Insulin Resistance, and Hepatic Lipid Accumulation in High-Fructose-Diet-Fed Rats" Nutrients 12, no. 12: 3762. https://0-doi-org.brum.beds.ac.uk/10.3390/nu12123762