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Article

Investigating Useful Properties of Four Streptomyces Strains Active against Fusarium graminearum Growth and Deoxynivalenol Production on Wheat Grains by qPCR

Department of Food, Environmental and Nutritional Science (DeFENS), University of Milan, 20133 Milan, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Received: 8 July 2020 / Revised: 20 August 2020 / Accepted: 26 August 2020 / Published: 31 August 2020
Streptomyces spp. can be exploited as biocontrol agents (BCAs) against plant pathogens such as Fusarium graminearum, the main causal agent of Fusarium head blight (FHB) and against the contamination of grains with deoxynivalenol (DON). In the present research, four Streptomyces strains active against F. graminearum in dual plate assays were characterized for their ability to colonize detached wheat grains in the presence of F. graminearum and to limit DON production. The pathogen and BCA abundance were assessed by a quantitative real-time PCR, while DON production was assessed by HPLC quantification and compared to ergosterol to correlate the toxin production to the amount of fungal mycelium. Fungal growth and mycotoxin production were assessed with both co-inoculation and late inoculation of the BCAs in vitro (three days post-Fusarium inoculation) to test the interaction between the fungus and the bacteria. The level of inhibition of the pathogen and the toxin production were strain-specific. Overall, a higher level of DON inhibition (up to 99%) and a strong reduction in fungal biomass (up to 71%) were achieved when streptomycetes were co-inoculated with the fungus. This research enabled studying the antifungal efficacy of the four Streptomyces strains and monitoring their development in DON-inducing conditions. View Full-Text
Keywords: biocontrol; mycotoxin; Triticum aestivum; qPCR; ergosterol biocontrol; mycotoxin; Triticum aestivum; qPCR; ergosterol
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MDPI and ACS Style

Colombo, E.M.; Kunova, A.; Gardana, C.; Pizzatti, C.; Simonetti, P.; Cortesi, P.; Saracchi, M.; Pasquali, M. Investigating Useful Properties of Four Streptomyces Strains Active against Fusarium graminearum Growth and Deoxynivalenol Production on Wheat Grains by qPCR. Toxins 2020, 12, 560. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090560

AMA Style

Colombo EM, Kunova A, Gardana C, Pizzatti C, Simonetti P, Cortesi P, Saracchi M, Pasquali M. Investigating Useful Properties of Four Streptomyces Strains Active against Fusarium graminearum Growth and Deoxynivalenol Production on Wheat Grains by qPCR. Toxins. 2020; 12(9):560. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090560

Chicago/Turabian Style

Colombo, Elena M., Andrea Kunova, Claudio Gardana, Cristina Pizzatti, Paolo Simonetti, Paolo Cortesi, Marco Saracchi, and Matias Pasquali. 2020. "Investigating Useful Properties of Four Streptomyces Strains Active against Fusarium graminearum Growth and Deoxynivalenol Production on Wheat Grains by qPCR" Toxins 12, no. 9: 560. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090560

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