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Article

Characterization of New Allergens from the Venom of the European Paper Wasp Polistes dominula

1
Center of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Helmholtz Center Munich, German Research Center for Environmental Health, 80802 Munich, Germany
2
Department of Dermatology and Allergy Biederstein, Technical University of Munich, 80802 Munich, Germany
3
Immunology Department, CDB Hospital Clinic de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, 08007 Barcelona, Spain
4
Spanish Network for Allergy—RETIC de Asma, Reacciones Adversas y Alérgicas (ARADYAL), 28040 Madrid, Spain
5
Immunology Department, CDB Hospital Clinic de Barcelona, Universitat de Barcelona, 08007 Barcelona, Spain
6
Allergy Section, Hospital Clinic de Barcelona, Clinical Medical Group, 08036 Barcelona, Spain
7
Department of Infection and Immunity, Luxembourg Institute of Health (LIH), 4354 Esch-Sur-Alzette, Luxembourg
8
Department of Dermatology and Allergy Center, Odense Research Center for Anaphylaxis, University of Southern Denmark, 5000 Odense, Denmark
9
Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60121 Ancona, Italy
10
Allergy Unit, Department of Internal Medicine, University Hospital of Ancona, 60126 Ancona, Italy
*
Author to whom correspondence should be addressed.
Received: 30 June 2021 / Revised: 27 July 2021 / Accepted: 6 August 2021 / Published: 10 August 2021
(This article belongs to the Special Issue Venom Allergy: General Concepts, Allergens, Diagnosis and Treatment)
Discriminating Polistes dominula and Vespula spp. venom allergy is of growing importance worldwide, as systemic reactions to either species’ sting can lead to severe outcomes. Administering the correct allergen-specific immunotherapy is therefore a prerequisite to ensure the safety and health of venom-allergic patients. Component-resolved diagnostics of Hymenoptera venom allergy might be improved by adding additional allergens to the diagnostic allergen panel. Therefore, three potential new allergens from P. dominula venom—immune responsive protein 30 (IRP30), vascular endothelial growth factor C (VEGF C) and phospholipase A2 (PLA2)—were cloned, recombinantly produced and biochemically characterized. Sera sIgE titers of Hymenoptera venom-allergic patients were measured in vitro to assess the allergenicity and potential cross-reactivity of the venom proteins. IRP30 and VEGF C were classified as minor allergens, as sensitization rates lay around 20–40%. About 50% of P. dominula venom-allergic patients had measurable sIgE titers directed against PLA2 from P. dominula venom. Interestingly, PLA2 was unable to activate basophils of allergic patients, questioning its role in the context of clinically relevant sensitization. Although the obtained results hint to a questionable benefit of the characterized P. dominula venom proteins for improved diagnosis of venom-allergic patients, they can contribute to a deeper understanding of the molecular mechanisms of Hymenoptera venoms and to the identification of factors that determine the allergenic potential of proteins. View Full-Text
Keywords: allergen; Hymenoptera venom allergy; phospholipase A2; Polistes dominula; allergen cross-reactivity allergen; Hymenoptera venom allergy; phospholipase A2; Polistes dominula; allergen cross-reactivity
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MDPI and ACS Style

Grosch, J.; Eberlein, B.; Waldherr, S.; Pascal, M.; San Bartolomé, C.; De La Roca Pinzón, F.; Dittmar, M.; Hilger, C.; Ollert, M.; Biedermann, T.; Darsow, U.; Bilò, M.B.; Schmidt-Weber, C.B.; Blank, S. Characterization of New Allergens from the Venom of the European Paper Wasp Polistes dominula. Toxins 2021, 13, 559. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13080559

AMA Style

Grosch J, Eberlein B, Waldherr S, Pascal M, San Bartolomé C, De La Roca Pinzón F, Dittmar M, Hilger C, Ollert M, Biedermann T, Darsow U, Bilò MB, Schmidt-Weber CB, Blank S. Characterization of New Allergens from the Venom of the European Paper Wasp Polistes dominula. Toxins. 2021; 13(8):559. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13080559

Chicago/Turabian Style

Grosch, Johannes, Bernadette Eberlein, Sebastian Waldherr, Mariona Pascal, Clara San Bartolomé, Federico De La Roca Pinzón, Michael Dittmar, Christiane Hilger, Markus Ollert, Tilo Biedermann, Ulf Darsow, Maria B. Bilò, Carsten B. Schmidt-Weber, and Simon Blank. 2021. "Characterization of New Allergens from the Venom of the European Paper Wasp Polistes dominula" Toxins 13, no. 8: 559. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13080559

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