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Article

The Effects of Zearalenone on the Localization and Expression of Reproductive Hormones in the Ovaries of Weaned Gilts

1
Department of Animal Sciences and Technology, Shandong Agricultural University, No. 61 Daizong Street, Tai’an 271018, China
2
Department of Life Sciences, Shandong Agricultural University, No. 61 Daizong Street, Tai’an 271018, China
*
Authors to whom correspondence should be addressed.
Received: 12 July 2021 / Revised: 22 August 2021 / Accepted: 5 September 2021 / Published: 7 September 2021
(This article belongs to the Special Issue Research on Pathogenic Fungi and Mycotoxins in China)
This study aims to investigate the effects of zearalenone (ZEA) on the localizations and expressions of follicle stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), gonadotropin releasing hormone (GnRH) and gonadotropin releasing hormone receptor (GnRHR) in the ovaries of weaned gilts. Twenty 42-day-old weaned gilts were randomly allocated into two groups, and treated with a control diet and a ZEA-contaminated diet (ZEA 1.04 mg/kg), respectively. After 7-day adjustment, gilts were fed individually for 35 days and euthanized for blood and ovarian samples collection before morning feeding on the 36th day. Serum hormones of E2, PRG, FSH, LH and GnRH were determined using radioimmunoassay kits. The ovaries were collected for relative mRNA and protein expression, and immunohistochemical analysis of FSHR, LHR, GnRH and GnRHR. The results revealed that ZEA exposure significantly increased the final vulva area (p < 0.05), significantly elevated the serum concentrations of estradiol, follicle stimulating hormone and GnRH (p < 0.05), and markedly up-regulated the mRNA and protein expressions of FSHR, LHR, GnRH and GnRHR (p < 0.05). Besides, the results of immunohistochemistry showed that the immunoreactive substances of ovarian FSHR, LHR, GnRH and GnRHR in the gilts fed the ZEA-contaminated diet were stronger than the gilts fed the control diet. Our findings indicated that dietary ZEA (1.04 mg/kg) could cause follicular proliferation by interfering with the localization and expression of FSHR, LHR, GnRH and GnRHR, and then affect the follicular development of weaned gilts. View Full-Text
Keywords: zearalenone; gilts; ovary; hormone zearalenone; gilts; ovary; hormone
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MDPI and ACS Style

Wan, B.; Yuan, X.; Yang, W.; Jiao, N.; Li, Y.; Liu, F.; Liu, M.; Yang, Z.; Huang, L.; Jiang, S. The Effects of Zearalenone on the Localization and Expression of Reproductive Hormones in the Ovaries of Weaned Gilts. Toxins 2021, 13, 626. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13090626

AMA Style

Wan B, Yuan X, Yang W, Jiao N, Li Y, Liu F, Liu M, Yang Z, Huang L, Jiang S. The Effects of Zearalenone on the Localization and Expression of Reproductive Hormones in the Ovaries of Weaned Gilts. Toxins. 2021; 13(9):626. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13090626

Chicago/Turabian Style

Wan, Boyang, Xuejun Yuan, Weiren Yang, Ning Jiao, Yang Li, Faxiao Liu, Mei Liu, Zaibin Yang, Libo Huang, and Shuzhen Jiang. 2021. "The Effects of Zearalenone on the Localization and Expression of Reproductive Hormones in the Ovaries of Weaned Gilts" Toxins 13, no. 9: 626. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13090626

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