Metabolomics Analysis Reveals Dynamic Accumulation of Sugar and Acid during Stem Development of Brassica juncea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Treatment
2.2. Methods for Determination of Morphological Indexes, Sugar and Acid Contents, and Related Enzymes
2.3. Metabolomics Analysis
2.3.1. Sample Preparation and UHPLC-MS/MS
2.3.2. Data Processing
2.3.3. Identification and Screening of Metabolites
2.4. Validation of Gene Expression Levels
3. Results
3.1. Morphological and Growth Index Changes during Stem Expansion of Stem Mustard
3.2. The Variation of Sugar and Acid Content during Stem Development
3.3. Analysis of the Correlation between Sugar and Acid Metabolizing Enzymes during the Development of Stem
3.4. Analysis of Metabolite Levels
3.4.1. Metabolic Data Evaluation
3.4.2. Analysis of Differential Metabolites
3.4.3. Analysis of Differential Metabolites
3.4.4. Analysis of Differential Metabolite Pathways
3.4.5. Analysis of Metabolites Related to Sugar and Acid Biosynthetic Pathways
3.5. The Expression Level of Genes Related to Sugar and Acid Biosynthesis Pathway by qPCR
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Xie, F.; Xuan, Z.; Chen, W.; Du, J.; Wang, Y.; Huo, E.; Guo, L.; Li, M. Metabolomics Analysis Reveals Dynamic Accumulation of Sugar and Acid during Stem Development of Brassica juncea. Agronomy 2022, 12, 3227. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy12123227
Xie F, Xuan Z, Chen W, Du J, Wang Y, Huo E, Guo L, Li M. Metabolomics Analysis Reveals Dynamic Accumulation of Sugar and Acid during Stem Development of Brassica juncea. Agronomy. 2022; 12(12):3227. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy12123227
Chicago/Turabian StyleXie, Fangjie, Zhengying Xuan, Wanqin Chen, Jiageng Du, Yalin Wang, Entong Huo, Ling Guo, and Mengyao Li. 2022. "Metabolomics Analysis Reveals Dynamic Accumulation of Sugar and Acid during Stem Development of Brassica juncea" Agronomy 12, no. 12: 3227. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy12123227