Study on Kinetics of Trans-Resveratrol, Total Phenolic Content, and Antioxidant Activity Increase in Vine Waste during Post-Pruning Storage
Round 1
Reviewer 1 Report
Dears Authors,
This paper studies the t-resveratrol, total phenolic contents and antioxidant capacity in different parts of the wine and as a function of the storage time. For me, the work is based on a topic that has already been extensively studied as shown by all the bibliography provided by the authors. However, in my opinion, nothing new to the subject is contributed. Therefore, the authors must emphasize what is the novelty that their work offers. In addition, the structure of the results and discussions makes reading a bit difficult, having to improve and order in another way.
For all these reasons, I consider that the article needs big improvements.
Observations:
Line 63. I do not see coherent explaining the stilbenes here. I would first explain stilbenes, then stilbenoid and finally t-resveratrol (From more to less).
Line 116-118. Beware of the use of "was" and "were".
Line 161. R2 put in superscript.
Line 170-171. Separate the numbers from the units.
Line 174. Put the equation of the calibration line for gallic acid and R2.
Table 1. What is the number in parentheses? what does “ns” and the “**” mean? Put a footnote explaining it.
Results in general. In my opinion, it is not very clear that some tables and figures represent the same results (Figure 1 and Table 1). Also, I would put the tables in the order that I then talk about in the text in the discussions section.
Figure 1. The values ​​that are represented in the figure are average values? If so, put the standard deviation. What days do those numbers correspond to? Sometimes they are in the middle of the points, other times down, other times up ... Also, I would not use this type of axis to represent days since it is an original variable and a bar diagram is more correct.
Figure 2. The values ​​that are represented in the figure are average values? If so, put the standard deviation. What days do those numbers correspond to? Sometimes they are in the middle of the points, other times down, other times up ... How often is data collected? Not the same days as for resveratrol? There is more data in this case than in Figure 1.
Figure 3. The values ​​that are represented in the figure are average values? If so, put the standard deviation.
Section 4.1. This section is a repetition of the same that is discussed in the following sections but all together. For me, this section should be included in the following sections because, in addition to repeating the same information, it confuses the reader since it seems that something new is being talked about.
Line 252. “181”. Where does the value 181 come from?
Line 259-260. The values ​​indicated in these lines do not appear in Figure 1.
Line 261-263. Information repeated in the previous paragraph.
Line 336 and line 348. You should not speak before Table 3 than Table 4.
Section 4.5. The increases in the TPC on specific days without any concrete holding seem very strange to me.
395-406 and Line 443-451. The information that appears in these paragraphs in the part of resveratrol is grouped in different sections. I would unify the way to describe these results: in a single section as for TPC and antioxidant activity or different sections for storage, disposal and size as for resveratrol.
Author Response
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Author Response File: 
Author Response.pdf
Reviewer 2 Report
There are many typos in the text as follows.
Page 2, Line 85: “3,5,40-trihydroxy-transstilbene” is “trans-3,5,4’-trihydroxystilbene”
Page 4, Line 117: “Merk” is “Merck”
Page 4, Line 121: “1 g/l” is “1 g/L”
Page 4, Line 152: “Schimadzu” is “Shimadzu”
Page 5, Line 168, Page 12, Line 364, and Page 14, Line 439: “et. al” is “et al.”
Page 5, Line 180: “2.5 ml” is “2.5 mL.”
Page 12, Line 346: “Trans-Resveratrol” is “Trans-resveratrol”
Page 12, Line 374~376: “Piacetanol [3,6], Apigenin, Ellagic acid [33], Viniferin, Trans-Vitisin, trans-Piceid [34], Gallic acid, Ellagic acid, p-Coumaric acid and others [35]” is “piacetanol [3,6], apigenin, ellagic acid [33], viniferin, trans-vitisin, trans-piceid [34], gallic acid, ellagic acid, p-coumaric acid and others [35]”
Page 13, Line 407: “3.6.” is “4.6.”
Page 5, Line 180~181: “The mixture (total volume of 10 mL)” is miscalculation?
Page 4, Line 157~158: The first gradient speed is very slow (from 0% to 10% B in 42 min), but second gradient speed (10-40% B in 42.6 min) and third speed (40-90% B in 46.5 min) are fast extremely. You should be indicated the typical chromatogram in this article.
Page 5, Table 1.: You should be shown the TRC and DPPH% of standard trans-resveratrol and discussed in “Discussion” section about comparison to samples and standard compound.
Page 13, Line 388~390: Generally, the phenolic compounds are not moved to aqueous layer by the use of sodium bicarbonate. You should explain another reasons about lower amount of TRC.
You are not discussing about the results of HPLC analysis in this article. You need to indicate the possible contained compounds such as the decreasing TRC and DPPH% from the HPLC analysis.
Author Response
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Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Dear Authors,
Most of the changes indicated have been made, and the comments have been consistently discussed, so the quality of the article has improved considerably.
However, in my opinion, the topic of this work continues to be widely studied by other authors, especially considering that no individual analysis of the total phenolic compounds (apart from resveratrol) have been carried out.
Author Response
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Author Response File: Author Response.pdf
Reviewer 2 Report
There are many typos in the text as follows.
Page 11, Line 286: “almost doubled (170.44 ± 3.82 mg / kg D.W)” is “almost doubled (151.60 ± 9.42 mg / kg D.W.)”
Page 11, Line 289: “with 151.60 ± 9.42 mg / kg D.W” is “with 50.41 ± 2.74 mg / kg D.W.)”
Page 11, Line 307: “bellow 20” is “bellow 20 °C”
Page 11, Line 308: “stored at -20” is “stored at -20 °C”
Page 13, Line 418: “stobenoid” is “stilbenoid”
Page 13, Line 437: “din” is “did”
Page 13~14, Line 443~447: “How ~ compound” was long sentence and understood hardly for readers.
Author Response
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Author Response File: Author Response.pdf
