High-Level Carbapenem Resistance among OXA-48-Producing Klebsiellapneumoniae with Functional OmpK36 Alterations: Maintenance of Ceftazidime/Avibactam Susceptibility
Abstract
:1. Introduction
2. Results and Discussion
2.1. Carbapenems and Ceftazidime/Avibactam Susceptibility, β-Lactamases and Molecular Epidemiology
2.2. Molecular Analysis of OmpK35 and OmpK36 in OXA-48 Producers
2.3. Disk Diffusion Assay Using Carbapenems and Cefoxitin for Detection of Functional Alteration in OmpK36:
3. Materials and Methods
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strain | ST | Sample | Previous Carbapenem Exposure (days) 1 | ESBL Enzyme | FOX MIC/IZD 2 | MER MIC/IZD 2 | ERT MIC/IZD2 | IPM vMIC/IZD 2 | CAZ/AVI MIC | ompK35 | ompK36 |
---|---|---|---|---|---|---|---|---|---|---|---|
Kp_HUCA_Bac_5 | 326 | Blood culture | Yes (10) | CTX-M-15 | >16/6 | >32/6 | >4/6 | >8/6 | 0.5 | WT | ::IS5 |
Kp_HUCA_Bac_7 | 326 | Blood culture | No | CTX-M-15 | >16/6 | 16/6 | >4/6 | >8/6 | 0.75 | WT | ::IS5 |
Kp_HUCA_Bac_10 | 326 | Blood culture | Yes (21) | CTX-M-15 | >16/6 | >32/8 | >4/6 | >8/9 | 0.5 | WT | ΔC201 |
Kp_HUCA_Bac_13 | 326 | Blood culture | Yes (11) | CTX-M-15 | >16/6 | >32/9 | >4/7 | >8/11 | 0.75 | WT | G259T |
Kp_HUCA_Bac_18 | 326 | Blood culture | No | CTX-M-15 | >16/7 | 32/8 | >4/6 | >8/9 | 0.5 | WT | ΔC201 |
Kp_HUCA_Bac_24 | 326 | Blood culture | Yes (5) | CTX-M-15 | >16/11 | >32/6 | >1/6 | >8/9 | 0.5 | ::623T | ::IS5 |
Kp_HUCA_Bac_64 | 16 | Blood culture | Yes (5) | CTX-M-15 | 16/11 | 8/16 | >4/16 | 8/18 | 0.5 | WT | ΔA74 |
Kp_HUCA_Bac_65 | 147 | Blood culture | No | SHV-12 | ≤8/15 | 8/16 | >1/16 | 4/19 | 0.38 | WT | ::IS5 |
Kp_HUCA_Bac_76 | 405 | Blood culture | Yes (7) | CTX-M-15 | >16/12 | >32/6 | >1/6 | >8/6 | 0.75 | ::623T | T612A |
Kp_HUCA_Bac_85 | 273 | Blood culture | Yes (19) | Non-ESBL | >16/6 | >32/6 | >1/6 | >8/6 | 0.5 | ::623T | ::29TG |
Kp_HUCA_Bac_88 | 405 | Blood culture | Yes (6) | Non-ESBL | 16/18 | 8/16 | >1/16 | 8/17 | 0.19 | WT | ::818ACAAAGCGCAGAACTTCGAACCTGGGCTTTGCAA |
Kp_HUCA_Bac_90 | 101 | Blood culture | Yes (2) | CTX-M-15 | >16/6 | 24/6 | >1/6 | 4/17 | 0.5 | ΔG185 | ::403GACGGC |
Kp_HUCA_2 | 326 | Rectal swab | No | CTX-M-15 | >16/6 | >32/6 | >1/6 | >8/8 | 0.5 | WT | G844T |
Kp_HUCA_3 | 15 | Sputum | No | CTX-M-15 | >16/6 | >32/6 | >1/6 | >8/14 | 0.75 | ::623T | ::403GACGGC |
Kp_HUCA_4 | 15 | Urine | Yes (15) | CTX-M-15 | >16/7 | >32/6 | >1/6 | >8/6 | 0.75 | WT | Δ(886)-184 |
Kp_HUCA_5 | 193 | Urine | No | Non-ESBL | >16/8 | 16/12 | >1/10 | >8/12 | 0.5 | WT | ::IS1 |
Kp_HUCA_8 | 326 | Rectal swab | Yes (4) | CTX-M-15 | >16/6 | 32/6 | >1/6 | >8/9 | 0.5 | WT | ΔA4 |
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Lumbreras-Iglesias, P.; Rodicio, M.R.; Valledor, P.; Suárez-Zarracina, T.; Fernández, J. High-Level Carbapenem Resistance among OXA-48-Producing Klebsiellapneumoniae with Functional OmpK36 Alterations: Maintenance of Ceftazidime/Avibactam Susceptibility. Antibiotics 2021, 10, 1174. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10101174
Lumbreras-Iglesias P, Rodicio MR, Valledor P, Suárez-Zarracina T, Fernández J. High-Level Carbapenem Resistance among OXA-48-Producing Klebsiellapneumoniae with Functional OmpK36 Alterations: Maintenance of Ceftazidime/Avibactam Susceptibility. Antibiotics. 2021; 10(10):1174. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10101174
Chicago/Turabian StyleLumbreras-Iglesias, Pilar, María Rosario Rodicio, Pablo Valledor, Tomás Suárez-Zarracina, and Javier Fernández. 2021. "High-Level Carbapenem Resistance among OXA-48-Producing Klebsiellapneumoniae with Functional OmpK36 Alterations: Maintenance of Ceftazidime/Avibactam Susceptibility" Antibiotics 10, no. 10: 1174. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10101174