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Open AccessArticle

Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus

1
Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt
2
Specialist of Laboratory Medical Analysis, Almokhtabar Private Laboratories, Zagazig 44511, Egypt
3
Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig 44511, Egypt
4
Fellow Pharmacist, Infection Control Unit, Zagazig University Hospital, Zagazig 44511, Egypt
5
Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh 11442, Saudi Arabia
6
Department of Biochemistry, Cancer Metabolism and Epigenetic Unit, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia
7
Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia
8
Department of Microbiology and Immunology, Faculty of Pharmacy, Port Said University, Port Said 42511, Egypt
*
Author to whom correspondence should be addressed.
Received: 25 August 2020 / Revised: 22 September 2020 / Accepted: 28 September 2020 / Published: 3 October 2020
(This article belongs to the Special Issue Nanoparticles-Based Antimicrobials)
Multidrug resistant (MDR) methicillin-resistant Staphylococcus aureus (MRSA) is a superbug pathogen that causes serious diseases. One of the main reasons for the lack of the effectiveness of antibiotic therapy against infections caused by this resistant pathogen is the recalcitrant nature of MRSA biofilms, which results in an increasingly serious situation worldwide. Consequently, the development of innovative biofilm inhibitors is urgently needed to control the biofilm formation by this pathogen. In this work, we thus sought to evaluate the biofilm inhibiting ability of some promising antibiofilm agents such as zinc oxide nanoparticles (Zno NPs), proteinase K, and hamamelitannin (HAM) in managing the MRSA biofilms. Different phenotypic and genotypic methods were used to identify the biofilm producing MDR MRSA isolates and the antibiofilm/antimicrobial activities of the used promising agents. Our study demonstrated strong antibiofilm activities of ZnO NPs, proteinase K, and HAM against MRSA biofilms along with their transcriptional modulation of biofilm (intercellular adhesion A, icaA) and quorum sensing (QS) (agr) genes. Interestingly, only ZnO NPs showed a powerful antimicrobial activity against this pathogen. Collectively, we observed overall positive correlations between the biofilm production and the antimicrobial resistance/agr genotypes II and IV. Meanwhile, there was no significant correlation between the toxin genes and the biofilm production. The ZnO NPs were recommended to be used alone as potent antimicrobial and antibiofilm agents against MDR MRSA and their biofilm-associated diseases. On the other hand, proteinase-K and HAM can be co-administrated with other antimicrobial agents to manage such types of infections. View Full-Text
Keywords: MRSA; antibiofilm; antimicrobial; ZnO-NPs; proteinase-K; HAM MRSA; antibiofilm; antimicrobial; ZnO-NPs; proteinase-K; HAM
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MDPI and ACS Style

Abd El-Hamid, M.I.; Y. El-Naenaeey, E.-s.; M kandeel, T.; Hegazy, W.A.H.; Mosbah, R.A.; Nassar, M.S.; Bakhrebah, M.A.; Abdulaal, W.H.; Alhakamy, N.A.; Bendary, M.M. Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus. Antibiotics 2020, 9, 667. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9100667

AMA Style

Abd El-Hamid MI, Y. El-Naenaeey E-s, M kandeel T, Hegazy WAH, Mosbah RA, Nassar MS, Bakhrebah MA, Abdulaal WH, Alhakamy NA, Bendary MM. Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus. Antibiotics. 2020; 9(10):667. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9100667

Chicago/Turabian Style

Abd El-Hamid, Marwa I.; Y. El-Naenaeey, El-sayed; M kandeel, Toka; Hegazy, Wael A.H.; Mosbah, Rasha A.; Nassar, Majed S.; Bakhrebah, Muhammed A.; Abdulaal, Wesam H.; Alhakamy, Nabil A.; Bendary, Mahmoud M. 2020. "Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus" Antibiotics 9, no. 10: 667. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9100667

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