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Article

Polyphasic Validation of a Nisin-Biogel to Control Canine Periodontal Disease

1
CIISA-Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal
2
Virbac de Portugal Laboratórios, Lda., Rua do Centro Empresarial, Quinta da Beloura, 2710-693 Sintra, Portugal
3
Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Received: 24 March 2020 / Revised: 11 April 2020 / Accepted: 13 April 2020 / Published: 14 April 2020
Background: Periodontal disease (PD) is a highly prevalent inflammatory disease in dogs. This disease is initiated by a polymicrobial biofilm on the teeth surface, whose control includes its prevention and removal. Recently, it was shown that nisin displays antimicrobial activity against canine PD-related bacteria. Moreover, guar gum biogel has shown to be a promising topical delivery system for nisin. Methods: In this study we aimed to evaluate the antimicrobial activity of the nisin-biogel in the presence of canine saliva and after a 24-month storage, at different conditions, using a canine oral enterococci collection. We also studied the nisin-biogel cytotoxicity using a Vero cell line and canine primary intestinal fibroblasts. Results: The presence of saliva hampers nisin-biogel antimicrobial activity, and higher nisin concentrations were required for an effective activity. A significant reduction (p ≤ 0.05) in inhibitory activity was observed for nisin-biogel solutions stored at 37 °C, over a 24-month period, which was not observed with the other conditions. The nisin-biogel showed no cytotoxicity against the cells tested at concentrations up to 200 µg/mL. Conclusions: Our results confirmed the potential of the nisin-biogel for canine PD control, supporting the development of an in vivo clinical trial. View Full-Text
Keywords: canine saliva; cell culture; dogs; nisin-biogel; periodontal disease; storage canine saliva; cell culture; dogs; nisin-biogel; periodontal disease; storage
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MDPI and ACS Style

Cunha, E.; Freitas, F.B.; São Braz, B.; Moreira da Silva, J.; Tavares, L.; Veiga, A.S.; Oliveira, M. Polyphasic Validation of a Nisin-Biogel to Control Canine Periodontal Disease. Antibiotics 2020, 9, 180. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9040180

AMA Style

Cunha E, Freitas FB, São Braz B, Moreira da Silva J, Tavares L, Veiga AS, Oliveira M. Polyphasic Validation of a Nisin-Biogel to Control Canine Periodontal Disease. Antibiotics. 2020; 9(4):180. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9040180

Chicago/Turabian Style

Cunha, Eva; Freitas, Ferdinando B.; São Braz, Berta; Moreira da Silva, Jorge; Tavares, Luís; Veiga, Ana S.; Oliveira, Manuela. 2020. "Polyphasic Validation of a Nisin-Biogel to Control Canine Periodontal Disease" Antibiotics 9, no. 4: 180. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9040180

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