Bioremediation of Polycyclic Aromatic Hydrocarbons from Industry Contaminated Soil Using Indigenous Bacillus spp.
Abstract
:1. Introduction
2. Research Design
3. Materials and Methods
3.1. Microbial Strain Selection
3.1.1. Sampling and Isolation
3.1.2. Molecular Identification
3.1.3. Strain Selection Based on Preliminary Hydrocarbon Degradation
3.2. High Cell Density Batch Cultivation
3.2.1. Fermentation Process for the Production of Hydrocarbon-Degrading Strains at 30 L Scale
3.2.2. Strain Recovery
3.3. Preparation of Mixed Culture Prototypes for Efficacy Testing
3.4. In Vitro Hydrocarbon Degradation Assessment
3.4.1. Assessment and Source of Contaminated Soil
3.4.2. Configuration and Setup of Bench-Scale Evaluation System
3.4.3. Monitoring and Evaluation of the Test Systems
3.4.4. Analyses
- (a)
- pH
- (b)
- Moisture content
- (c)
- PAH concentration
- (d)
- Statistical methods
4. Results and Discussion
4.1. Microbial Strain Identification and Preliminary Assessment of Hydrocarbon Degradation
4.2. High Cell Density Cultivations of Selected Strains
4.3. In Vitro Hydrocarbon Degradation Assessment of Prototypes Containing Mixed Cultures
4.3.1. Assessment of PAH Contaminants in Soil
4.3.2. Removal of Polycyclic Aromatic Hydrocarbons in Test Systems
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Andrade, J.M.; Estévez-Pérez, M.G. Statistical comparison of the slopes of two regression lines: A tutorial. Anal. Chim. Acta 2014, 838, 1–12. [Google Scholar] [CrossRef]
- Atlas, R.M. Microbial Degradation of Petroleum Hydrocarbons: An Environmental Perspective. Microbiol. Rev. 1981, 45, 180–209. [Google Scholar] [CrossRef]
- Chikere, C.B.; Okpokwasili, G.C.; Chikere, B.O. Monitoring of microbial hydrocarbon remediation in the soil. 3 Biotech 2011, 1, 117–138. [Google Scholar] [CrossRef] [Green Version]
- Das, N.; Chandran, P. Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview. Biotechnol. Res. Int. 2011, 2011, 941810. [Google Scholar] [CrossRef] [Green Version]
- Department of Environmental Affairs National Norms and Standards for the Remediation of Contaminated Land and Soil Quality. Gov. Gaz. 2012, 561, 1–16.
- Escobar, V.V.; López, S.M.; Uribe, L.F.P.; Correa, E.M.R.; Gaviria, T.Z.C.; Castillo, J.J.M.; Roldán, L.E.A. Process for Increasing Biomass and Spores Production of Plant Growth Promoting Bacteria of the Bacillus Genus. U.S. Patent No. 9,839,222, 12 December 2017. [Google Scholar]
- Gałazka, A.; Gałazka, R. Phytoremediation of polycyclic aromatic hydrocarbons in soils artificially polluted using plant-associated-endophytic bacteria and Dactylis glomerata as the bioremediation plant. Pol. J. Microbiol. 2015, 64, 241–252. [Google Scholar] [CrossRef]
- Ghazali, F.M.; Rahman, R.N.Z.A.; Salleh, A.B.; Basri, M. Biodegradation of hydrocarbons in soil by microbial consortium. Int. Biodeterior. Biodegrad. 2004, 54, 61–67. [Google Scholar] [CrossRef]
- Ghosal, D.; Ghosh, S.; Dutta, T.K.; Ahn, Y. Current state of knowledge in microbial degradation of polycyclic aromatic hydrocarbons (PAHs): A review. Front. Microbiol. 2016, 7, 1837. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Giunta, M.; Lo Bosco, D.; Leonardi, G.; Scopelliti, F. Estimation of gas and dust emissions in construction sites of a motorway project. Sustainability 2019, 11, 7218. [Google Scholar] [CrossRef] [Green Version]
- Gkorezis, P.; Daghio, M.; Franzetti, A.; Van Hamme, J.D.; Sillen, W.; Vangronsveld, J. The interaction between plants and bacteria in the remediation of petroleum hydrocarbons: An environmental perspective. Front. Microbiol. 2016, 7, 1836. [Google Scholar] [CrossRef] [PubMed]
- Gupta, G.; Kumar, V.; Pal, A.K. Microbial Degradation of High Molecular Weight Polycyclic Aromatic Hydrocarbons with Emphasis on Pyrene. Polycyclic Aromatic Compounds. Taylor Fr. 2019, 39, 124–138. [Google Scholar] [CrossRef]
- Habib, S.; Johari, W.L.W.; Shukor, M.Y.; Yasid, N.A. Screening of Hydrocarbon-degrading Bacterial Isolates Using the Redox Application of 2, 6-DCPIP. Bioremediat. Sci. Technol. 2017, 5, 13–16. [Google Scholar]
- Hamamura, N.; Olson, S.H.; Ward, D.M.; Inskeep, W.P. Microbial population dynamics associated with crude-oil biodegradation in diverse soils. Appl. Environ. Microbiol. 2006, 72, 6316–6324. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Haritash, A.K.; Kaushik, C.P. Biodegradation aspects of Polycyclic Aromatic Hydrocarbons (PAHs): A review. J. Hazard. Mater. 2009, 169, 1–15. [Google Scholar] [CrossRef] [PubMed]
- Ichor, T.; Okerentugba, P.; Okpokwasili, G. Biodegradation of Total Petroleum Hydrocarbon by Aerobic Heterotrophic Bacteria Isolated from Crude Oil Contaminated Brackish Waters of Bodo Creek. J. Bioremediat. Biodegrad. 2014, 5, 1–6. [Google Scholar] [CrossRef]
- Johnson, O.A.; Affam, A.C. Petroleum sludge treatment and disposal: A review. Environ. Eng. Res. 2019, 24, 191–201. [Google Scholar] [CrossRef]
- Kelly, D.P.; Ardley, J.K.; Wood, A.P. Cultivation of Methylotrophs. In Hydrocarbon and Lipid Microbiology Protocols—Springer Protocols Handbooks; Springer: Berlin/Heidelberg, Germany, 2014; pp. 249–268. [Google Scholar] [CrossRef]
- Kim, J.D.; Lee, C.G. Microbial degradation of polycyclic aromatic hydrocarbons in soils by bacterium-fungus co-cultures. Biotechnol. Bioprocess Eng. 2007, 12, 410–416. [Google Scholar] [CrossRef]
- Lalloo, R.; Ramchuran, S.; Ramduth, D.; Görgens, J.; Gardiner, N. Isolation and selection of Bacillus sas potential biological agents for enhancement of water quality in culture of ornamental fish. J. Appl. Microbiol. 2007, 103, 1471–1479. [Google Scholar] [CrossRef]
- Lawal, A.T. Polycyclic aromatic hydrocarbons: A review. Cogent Environmental Science. Cogent 2017, 3, 537–567. [Google Scholar] [CrossRef]
- Leahy, J.G.; Colwell, R.R. Microbial degradation of hydrocarbons in the environment. Microbiol. Rev. 1990, 54, 305–315. [Google Scholar] [CrossRef]
- Lee, K.C.; Darah, I.; Ibrahim, C.O. A laboratory scale bioremediation of Tapis crude oil contaminated soil bybioaugmentation of Acinetobacter baumannii T30C. Afr. J. Microbiol. Res. 2016, 5, 2609–2615. [Google Scholar] [CrossRef]
- Lei, Y.J.; Zhang, J.; Tian, Y.; Yao, J.; Duan, Q.S.; Zuo, W. Enhanced degradation of total petroleum hydrocarbons in real soil by dual-frequency ultrasound-activated persulfate. Sci. Total. Environ. 2020, 748, 141414. [Google Scholar] [CrossRef] [PubMed]
- Ma, J.; Xu, L.; Jia, L. Degradation of polycyclic aromatic hydrocarbons by Pseudomonas sp. JM2 isolated from active sewage sludge of chemical plant. J. Environ. Sci. 2012, 24, 2141–2148. [Google Scholar] [CrossRef]
- MacLeod, C.T.; Daugulis, A.J. Interfacial effects in a two-phase partitioning bioreactor: Degradation of polycyclic aromatic hydrocarbons (PAHs) by a hydrophobic Mycobacterium. Process. Biochem. 2005, 40, 1799–1805. [Google Scholar] [CrossRef]
- Masika, W.S.; Moonsamy, G.; Mandree, P.; Ramchuran, S.; Lalloo, R.; Kudanga, T. Biodegradation of petroleum hydrocarbon waste using consortia of Bacillus sp. Bioremediat. J. Taylor Fr. 2020, 25, 72–79. [Google Scholar] [CrossRef]
- Mohandass, R.; Rout, P.; Jiwal, S.; Sasikala, C. Biodegradation of Benzo[a]pyrene by the mixed culture of Bacillus cereus and Bacillus vlretl isolated from the petrochemical industry. J. Environ. Biol. 2012, 33, 985–989. [Google Scholar]
- Nicholson, W.L.; Munakata, N.; Horneck, G.; Melosh, H.J.; Setlow, P. Resistance of Bacillus Endospores to Extreme Terrestrial and Extraterrestrial Environments. Microbiol. Mol. Biol. Rev. 2000, 64, 548–572. [Google Scholar] [CrossRef] [Green Version]
- Obayori, O.S.; Salam, L.B. Degradation of polycyclic aromatic hydrocarbons: Role of plasmids. Sci. Res. Essays 2010, 5, 4096–4109. [Google Scholar]
- Pogodda, S. Requirements for Discharge of Trade Effluent into the Public Sewers. 2013; p. 18571442. Available online: https://www.pub.gov.sg/Documents/requirements_UW.pdf (accessed on 4 July 2018).
- Rabodonirina, S.; Rasolomampianina, R.; Krier, F.; Drider, D.; Merhaby, D.; Net, S.; Ouddane, B. Degradation of fluorene and phenanthrene in PAHs-contaminated soil using Pseudomonas and Bacillus strains isolated from oil spill sites. J. Environ. Manag. 2019, 232, 1–7. [Google Scholar] [CrossRef]
- Seo, J.-S.; Keom, Y.-S.; Li, Q.X. Bacterial degradation of aromatic compounds. Int. J. Environ. Res. Public Health 2009, 6, 278–309. [Google Scholar] [CrossRef]
- Sooch, B.S.; Kauldhar, B.S.; Puri, M. Types, Structure, Applications and Future Outlook. Microb. Enzym. Technol. Food Appl. 2020, 241–254. [Google Scholar] [CrossRef]
- Tarafdar, A.; Sinha, A.; Masto, R.E. Biodegradation of anthracene by a newly isolated bacterial strain, Bacillus thuringiensis AT.ISM.1, isolated from a fly ash deposition site. Lett. Appl. Microbiol. 2017, 65, 327–334. [Google Scholar] [CrossRef] [PubMed]
- USEPA Method 9045D: Soil and Waste pH. United States Environmental Protection Agency, (November). Available online: http://biblioteca.usac.edu.gt/tesis/08/08_2469_C.pdf (accessed on 6 July 2018).
- USEPA Method 8270E: Semivolatile Organic Compounds by Gas Chromatography/Mass Spectrometry (GC/MS). United States Environmental Protection Agency. 2018. Available online: https://www.epa.gov/sites/default/files/2020-10/documents/method_8270e_update_vi_06-2018_0.pdf (accessed on 8 July 2018).
- Wang, W.; Wang, L.; Shao, Z. Polycyclic aromatic hydrocarbon (PAH) degradation pathways of the obligate marine PAH degrader Cycloclasticus sp. strain P1. Appl. Environ. Microbiol. 2018, 84, e01261-18. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Strain | Molecular Identification | TRPH (%) |
---|---|---|
GPA7.1 | Bacillus velezensis | 92.94 ± 1.23 |
GPA11.2 | Bacillus subtilis | 84.86 ± 1.84 |
GPA6.2 | Bacillus velezensis | 95.99 ± 0.48 |
P401 | Bacillus cereus | 65.65 ± 6.30 |
D014 | Bacillus subtilis | 78.83 ± 4.50 |
P402 | Bacillus subtilis | 78.83 ± 4.50 |
Strain | Final Cell Concentration (Cell·mL−1) | Sporulation Efficiency (%) |
---|---|---|
GPA7.1 | 2.29 × 1010 | 90 |
GPA11.2 | 1.62 × 1010 | 91 |
GPA6.2 | 1.51 × 1010 | 76 |
P401 | 2.10 × 1010 | 95 |
D014 | 3.3 × 1010 | 92 |
P402 | 1.84 × 1010 | 81 |
Hydrocarbon | Concentration (µg·kg−1) | Chemical Description | Molecular Weight (g·mol−1) |
---|---|---|---|
M+P Xylene | 19 | 106.16 | |
O-Xylene | 18 | 106.16 | |
1,2,4 Trimethyl benzene | 19 | 120.19 | |
Naphthalene | 29 | 128.17 | |
Phenanthrene | 46 | 178.23 | |
Fluoranthene | 34 | 202.25 | |
Pyrene | 70 | 202.26 |
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Mandree, P.; Masika, W.; Naicker, J.; Moonsamy, G.; Ramchuran, S.; Lalloo, R. Bioremediation of Polycyclic Aromatic Hydrocarbons from Industry Contaminated Soil Using Indigenous Bacillus spp. Processes 2021, 9, 1606. https://0-doi-org.brum.beds.ac.uk/10.3390/pr9091606
Mandree P, Masika W, Naicker J, Moonsamy G, Ramchuran S, Lalloo R. Bioremediation of Polycyclic Aromatic Hydrocarbons from Industry Contaminated Soil Using Indigenous Bacillus spp. Processes. 2021; 9(9):1606. https://0-doi-org.brum.beds.ac.uk/10.3390/pr9091606
Chicago/Turabian StyleMandree, Prisha, Wendy Masika, Justin Naicker, Ghaneshree Moonsamy, Santosh Ramchuran, and Rajesh Lalloo. 2021. "Bioremediation of Polycyclic Aromatic Hydrocarbons from Industry Contaminated Soil Using Indigenous Bacillus spp." Processes 9, no. 9: 1606. https://0-doi-org.brum.beds.ac.uk/10.3390/pr9091606