Sargahydroquinoic Acid Suppresses Hyperpigmentation by cAMP and ERK1/2-Mediated Downregulation of MITF in α-MSH-Stimulated B16F10 Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Plant Material, Extraction, and Isolation of SHQA
2.3. Cell Culture, Treatment, and Viability Assay
2.4. Melanin Content Assay
2.5. Intracellular cAMP Assay
2.6. Immunoprecipitation
2.7. Protein-Ligand Docking Simulation
2.8. Statistical Analysis
3. Results
3.1. SHQA Inhibits Cellular TYR Activity and Melanin Production in B16F10 Cells
3.2. SHQA Suppresses the Production of Melanogenic Enzymes TYR and TRP1, and Their Transcription Factor MITF
3.3. SHQA Inhibits CREB Activation in B16F10 Cells
3.4. SHQA Suppresses Cellular cAMP Production and PKA Activation
3.5. SHQA May Have Binding Affinity to PKA (RIIβ)
3.6. SHQA Induces Proteasome-Mediated Degradation of MITF via ERK1/2 Activation
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Azam, M.S.; Kim, J.-I.; Choi, C.G.; Choi, J.; Lee, B.; Kim, H.-R. Sargahydroquinoic Acid Suppresses Hyperpigmentation by cAMP and ERK1/2-Mediated Downregulation of MITF in α-MSH-Stimulated B16F10 Cells. Foods 2021, 10, 2254. https://0-doi-org.brum.beds.ac.uk/10.3390/foods10102254
Azam MS, Kim J-I, Choi CG, Choi J, Lee B, Kim H-R. Sargahydroquinoic Acid Suppresses Hyperpigmentation by cAMP and ERK1/2-Mediated Downregulation of MITF in α-MSH-Stimulated B16F10 Cells. Foods. 2021; 10(10):2254. https://0-doi-org.brum.beds.ac.uk/10.3390/foods10102254
Chicago/Turabian StyleAzam, Mohammed Shariful, Jae-Il Kim, Chang Geun Choi, Jinkyung Choi, Bonggi Lee, and Hyeung-Rak Kim. 2021. "Sargahydroquinoic Acid Suppresses Hyperpigmentation by cAMP and ERK1/2-Mediated Downregulation of MITF in α-MSH-Stimulated B16F10 Cells" Foods 10, no. 10: 2254. https://0-doi-org.brum.beds.ac.uk/10.3390/foods10102254