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Open AccessArticle

Effects of Chemically-Functionalized Single-Walled Carbon Nanotubes on the Morphology and Vitality of D54MG Human Glioblastoma Cells

1
Department of Biology, The University of Alabama at Birmingham, Birmingham, AL 35294, USA
2
Department of Neurobiology, The University of Alabama at Birmingham, Birmingham, AL 35294, USA
3
Department of Biomedical Engineering and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
4
Department of Neuroscience, Center for Brain and Spinal Cord Repair and Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA
5
Departments of Chemistry and Chemical Engineering and Center for Nanoscale Science and Engineering, University of California, Riverside, CA 92521, USA
6
Carbon Solutions, Inc., Riverside, CA 92507, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Deceased on 21 April 2016.
Received: 30 August 2018 / Revised: 2 October 2018 / Accepted: 8 October 2018 / Published: 16 October 2018
The unique properties of single-walled carbon nanotubes (SWCNTs) have made them interesting candidates for applications in biomedicine. There are diverse chemical groups that can be attached to SWCNTs in order for these tiny tubes to gain various functionalities, for example, water solubility. Due to the availability of these “functionalization” approaches, SWCNTs are seen as agents for a potential anti-cancer therapy. In this context, we tested different chemically-functionalized forms of SWCNTs to determine which modifications make them better combatants against glioblastoma (astrocytoma grade IV), the deadliest brain cancer. We investigated the effects that two types of water soluble SWCNTs, functionalized with polyethylene glycol (SWCNT-PEG) or tetrahydrofurfuryl-terminated polyethylene glycol (SWCNT-PEG-THFF), have on the morphology and vitality, that is, cell adhesion, proliferation and death rate, of the D54MG human glioblastoma cells in culture. We found that SWCNT-PEG-THFF solute, when added to culture media, makes D54MG cells less round (measured as a significant decrease, by ~23%, in the form factor). This morphological change was induced by the PEG-THFF functional group, but not the SWCNT backbone itself. We also found that SWCNT-PEG-THFF solute reduces the proliferation rate of D54MG cells while increasing the rate of cell death. The functional groups PEG and PEG-THFF, on the other hand, reduce the cell death rate of D54MG human glioma cells. These data indicate that the process of functionalization of SWCNTs for potential use as glioma therapeutics may affect their biological effects. View Full-Text
Keywords: glioblastoma multiforme; carbon nanotubes; morphology; cell adhesion; cell proliferation; cell death rate glioblastoma multiforme; carbon nanotubes; morphology; cell adhesion; cell proliferation; cell death rate
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MDPI and ACS Style

Hopkins, S.; Gottipati, M.K.; Montana, V.; Bekyarova, E.; Haddon, R.C.; Parpura, V. Effects of Chemically-Functionalized Single-Walled Carbon Nanotubes on the Morphology and Vitality of D54MG Human Glioblastoma Cells. Neuroglia 2018, 1, 327-338. https://0-doi-org.brum.beds.ac.uk/10.3390/neuroglia1020022

AMA Style

Hopkins S, Gottipati MK, Montana V, Bekyarova E, Haddon RC, Parpura V. Effects of Chemically-Functionalized Single-Walled Carbon Nanotubes on the Morphology and Vitality of D54MG Human Glioblastoma Cells. Neuroglia. 2018; 1(2):327-338. https://0-doi-org.brum.beds.ac.uk/10.3390/neuroglia1020022

Chicago/Turabian Style

Hopkins, Seantel; Gottipati, Manoj K.; Montana, Vedrana; Bekyarova, Elena; Haddon, Robert C.; Parpura, Vladimir. 2018. "Effects of Chemically-Functionalized Single-Walled Carbon Nanotubes on the Morphology and Vitality of D54MG Human Glioblastoma Cells" Neuroglia 1, no. 2: 327-338. https://0-doi-org.brum.beds.ac.uk/10.3390/neuroglia1020022

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