Control of Surface Properties of Hyaluronan/Chitosan Multilayered Coatings for Tumor Cell Capture
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Polyelectrolyte Solutions
2.2.2. Preparation of Substrates
2.2.3. Film Assembly via Layer-by-Layer Deposition
2.2.4. Cell Adhesion Assays
2.2.5. Physicochemical Characterization
3. Results and Discussion
3.1. Film Characterization
3.1.1. Profilometry
3.1.2. UV-Vis
3.1.3. Contact Angle
3.1.4. Atomic Force Microscopy (AFM)
3.1.5. Capacitance
3.2. Cell Adhesion Assays
Selective Potential of the Multilayer Films
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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pH Condition | Number of Bilayers | Roughness (nm) |
---|---|---|
3.00 | 3.5 | 13 ± 3 |
10.5 | 44 ± 12 | |
20.5 | 42 ± 6 | |
5.00 | 3.5 | 8 ± 1 |
10.5 | 23 ± 4 | |
20.5 | 21 ± 6 |
pH Condition | Number of Bilayers | Capacitance (mV) |
---|---|---|
3.00 | 3.5 | 119 ± 23 |
10.5 | 122 ± 29 | |
20.5 | 150 ± 10 | |
5.00 | 3.5 | 155 ± 52 |
10.5 | 152 ± 47 | |
20.5 | 146 ± 10 |
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Lima, G.G.; Rocha Neto, J.B.M.; Carvalho, H.F.d.; Beppu, M.M. Control of Surface Properties of Hyaluronan/Chitosan Multilayered Coatings for Tumor Cell Capture. Polysaccharides 2021, 2, 387-399. https://0-doi-org.brum.beds.ac.uk/10.3390/polysaccharides2020025
Lima GG, Rocha Neto JBM, Carvalho HFd, Beppu MM. Control of Surface Properties of Hyaluronan/Chitosan Multilayered Coatings for Tumor Cell Capture. Polysaccharides. 2021; 2(2):387-399. https://0-doi-org.brum.beds.ac.uk/10.3390/polysaccharides2020025
Chicago/Turabian StyleLima, Giulia G., João B. M. Rocha Neto, Hernandes Faustino de Carvalho, and Marisa Masumi Beppu. 2021. "Control of Surface Properties of Hyaluronan/Chitosan Multilayered Coatings for Tumor Cell Capture" Polysaccharides 2, no. 2: 387-399. https://0-doi-org.brum.beds.ac.uk/10.3390/polysaccharides2020025