Animal Herpesvirus Infections

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Veterinary Clinical Studies".

Deadline for manuscript submissions: closed (10 July 2023) | Viewed by 1415

Special Issue Editors


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Guest Editor
Virology Laboratory, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Prédio UFRGS nº 21116 - Sala 523, Porto Alegre, Brazil CEP 90035-003
Interests: Bovine herpesviruses

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Guest Editor
Virology Laboratory, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Prédio UFRGS nº 21116 - Sala 523, Porto Alegre, Brazil
Interests: bovine herpesvirus; bubaline herpesvirus
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Special Issue Information

Dear Colleagues,

Animal herpesviruses can infect either domestic or wild animals, mostly in a species-specific manner. These viruses are prevalent and widespread pathogens that are able to establish lifelong latent infections in different cell types, depending on the virus species and host factors. Latency is a unique example of a fascinating type of virus–host interaction and a result of a long co-evolution process between virus and host. Understanding viral evolution, epidemiology, virus–cell interactions, virus pathogenesis, immune response and pathology caused by these pathogens is crucial for the development of new tools for the control and/or treatment of such infections. Moreover, herpesviruses have been used as effective vectors to convey heterologous immunogens targeting several pathogens of different species. This Special Issue of Animals will provide reviews, contemporary research reports, and commentaries about these fascinating pathogens and a platform for important contributions to the current knowledge on animal herpesviruses.

Dr. Ana Cláudia Franco
Dr. Fabrício S. Campos
Guest Editors

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Keywords

  • herpesvirus
  • alphaherpesvirus
  • betaherpesvirus
  • gammaherpesvirus
  • latency
  • epidemiology
  • pathogenesis
  • immune response
  • vaccine

Published Papers (1 paper)

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Research

14 pages, 2962 KiB  
Article
Molecular Characterization of UL50 (dUTPase) Gene of Bovine Herpes Virus 1
by Farzana Shahin, Sohail Raza, Xi Chen, Changmin Hu, Yingyu Chen, Huanchun Chen and Aizhen Guo
Animals 2023, 13(16), 2607; https://0-doi-org.brum.beds.ac.uk/10.3390/ani13162607 - 12 Aug 2023
Viewed by 1084
Abstract
Bovine herpes virus -1 (BoHV-1) infection leads to upper respiratory tract infection, conjunctivitis and genital disorders in cattle. To control BoHV-1, it is important to understand the role of viral proteins in viral infection. BoHV-1 has several gene products to help in viral [...] Read more.
Bovine herpes virus -1 (BoHV-1) infection leads to upper respiratory tract infection, conjunctivitis and genital disorders in cattle. To control BoHV-1, it is important to understand the role of viral proteins in viral infection. BoHV-1 has several gene products to help in viral replication in infected cell. One such gene is deoxyuridine triphosphate nucleotidohydrolase (dUTPase) also known as UL50. In this study, we analyzed the amino acid sequence of UL50 (dUTPase) using bioinformatics tools and found that it was highly conserved among herpesvirus family. Then, it was cloned and expressed in Escherichia coli Rosetta (DE3), induced by isopropy1-b-D-thiogalactopyranoside (IPTG) and the recombinant UL50 protein was purified to immunize rabbits for the preparation of polyclonal antiserum. The results indicated that the UL50 gene of BoHV-1 was composed of 978 nucleotides, which encoded 323 amino acids. Western blot analysis revealed that polyclonal sera against UL50 reacted with a band of 34 kDa. Furthermore, immunofluorescence assay showed that UL50 localized in the cytoplasmic area. Taken together, UL50 was successfully cloned, expressed and detected in BoHV-1-infected cells and was localized in the cytoplasm to help in the replication of BoHV-1 in infected cells. Full article
(This article belongs to the Special Issue Animal Herpesvirus Infections)
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