Probes for Biosensing and Bioimaging

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Optical and Photonic Biosensors".

Deadline for manuscript submissions: 31 July 2024 | Viewed by 2082

Special Issue Editors


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Guest Editor
College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, China
Interests: fluorescent probe; in situ imaging; signal amplification

E-Mail Website
Guest Editor
Department of Chemistry, Hubei University, Wuhan, China
Interests: fluorescent probe; molecular theranostics; in vivo imaging

Special Issue Information

Dear Colleagues,

With the continuous development of biosensing and bioimaging technologies, fluorescent probes with the advantages of high sensitivity, selectivity, rapid responses and real-time detection have achieved remarkable achievements in food safety, environmental monitoring, disease diagnosis, and so on, and have exhibited great potential for various applications. The use of molecular fluorescent probes to monitor life activity events is of great importance to gain insight into relevant physiological and pathological processes, disease diagnosis and treatment. Molecular probe-based fluorescence imaging technology has become a powerful tool for monitoring disease biomarkers. It is believed that more biosensing and bioimaging probes will be taken from the laboratory to the practical field. Therefore, this Special Issue is dedicated to gathering significant advances in innovative research on the various probes available for biosensing and bioimaging and their various applications.

Dr. Hongwen Liu
Dr. Zhiqiang Mao
Guest Editors

Manuscript Submission Information

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Keywords

  • fluorescent probe
  • small-molecular probe
  • biosensing
  • bioimaging

Published Papers (1 paper)

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Review

34 pages, 10252 KiB  
Review
Fluorescent Probes for Mammalian Thioredoxin Reductase: Mechanistic Analysis, Construction Strategies, and Future Perspectives
by Zilong Song, Chengwu Fan, Jintao Zhao, Lei Wang, Dongzhu Duan, Tong Shen and Xinming Li
Biosensors 2023, 13(8), 811; https://0-doi-org.brum.beds.ac.uk/10.3390/bios13080811 - 13 Aug 2023
Viewed by 1439
Abstract
The modulation of numerous signaling pathways is orchestrated by redox regulation of cellular environments. Maintaining dynamic redox homeostasis is of utmost importance for human health, given the common occurrence of altered redox status in various pathological conditions. The cardinal component of the thioredoxin [...] Read more.
The modulation of numerous signaling pathways is orchestrated by redox regulation of cellular environments. Maintaining dynamic redox homeostasis is of utmost importance for human health, given the common occurrence of altered redox status in various pathological conditions. The cardinal component of the thioredoxin system, mammalian thioredoxin reductase (TrxR) plays a vital role in supporting various physiological functions; however, its malfunction, disrupting redox balance, is intimately associated with the pathogenesis of multiple diseases. Accordingly, the dynamic monitoring of TrxR of live organisms represents a powerful direction to facilitate the comprehensive understanding and exploration of the profound significance of redox biology in cellular processes. A number of classic assays have been developed for the determination of TrxR activity in biological samples, yet their application is constrained when exploring the real-time dynamics of TrxR activity in live organisms. Fluorescent probes offer several advantages for in situ imaging and the quantification of biological targets, such as non-destructiveness, real-time analysis, and high spatiotemporal resolution. These benefits facilitate the transition from a poise to a flux understanding of cellular targets, further advancing scientific studies in related fields. This review aims to introduce the progress in the development and application of TrxR fluorescent probes in the past years, and it mainly focuses on analyzing their reaction mechanisms, construction strategies, and potential drawbacks. Finally, this study discusses the critical challenges and issues encountered during the development of selective TrxR probes and proposes future directions for their advancement. We anticipate the comprehensive analysis of the present TrxR probes will offer some glitters of enlightenment, and we also expect that this review may shed light on the design and development of novel TrxR probes. Full article
(This article belongs to the Special Issue Probes for Biosensing and Bioimaging)
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