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Biosensors
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Advances in Amplification Methods for Biosensors (Volume II)
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Advances in Amplification Methods for Biosensors (Volume II)

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (30 September 2023) | Viewed by 2930

Special Issue Editor

Dr. Arnaud Buhot

E-Mail Website
Guest Editor
Grenoble Alpes University, CEA, CNRS, IRIG-SyMMES, 17 Rue des Martyrs, 38000 Grenoble, France
Interests: optoelectronic nose/tongue development; aptamer biosensors; surface plasmons resonance imaging; theory of microarrays (DNA or protein); biopolymer conformation; DNA based architectures; soft condensed matter
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Today, there is rapidly growing demand for sensitive and selective biosensors in various domains, including environment monitoring such as (waste)water control, detection of pollution for personal/public safety, agricultural/food safety and quality control, veterinary and medical diagnostics, etc. For these applications, the main challenge remains to detect a minute number of analytes in complex samples. Thus, recent biosensors based on biomolecular recognition between analyte targets and relevant probes (antibodies, aptamers, molecular imprinted polymers (MIP), etc.) require the use of amplification methods to produce a measurable signal. The COVID-19 pandemic is a perfect demonstration of the need for (nucleic acid) amplification methods for the detection of the SARS-CoV2 virus (RT-PCR, RT-LAMP, ELISA, etc.).

Therefore, this Special Issue of Biosensors will highlight recent advances in the design and development of novel amplification methods to improve the performance of biosensors. All potential analyte targets will be considered, ranging from molecules (ions, pesticides, hormones, antibiotics, endocrine disruptors, miRNA, proteins, biomarkers, etc.) to larger objects (viruses, spores, fungus, bacteria, cancer cells, etc.). Biosensors based on optical, electrochemical, chemiluminescence, fluorescence, resonant or mechanical transduction methods are encouraged. Finally, amplification methods are not restricted: nucleic acid amplification (PCR, LAMP, RCA, NASBA, HCR, etc.) or logic gate circuits, enzymatic amplification (enzymes, DNAzymes, CRISPR, etc.), nanostructure-based amplification (nanoparticles, nanotubes, nanovesicles, MEMS/NEMS, nanosensors, etc.), as well as combined strategies implying several amplifications.

Dr. Arnaud Buhot
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • amplification
  • biosensors
  • electrochemical sensors
  • chemiluminescence sensors
  • optical sensors
  • resonant sensors
  • fluorescence sensors
  • nanosensors
  • nanoparticles
  • nanotubes
  • MEMS and/or NEMS
  • enzymes
  • DNAzymes
  • nucleic acid amplification
  • polymer chain reaction (PCR)
  • loop-mediated isothermal amplification (LAMP)
  • rolling circle amplification (RCA)
  • nucleic acid sequence-based amplification (NASBA)
  • strand displacement amplification (SDA)
  • hybridization chain reaction (HCR)
  • recombinase polymerase amplification (RPA)
  • logic gate circuits
  • sensor analysis
  • environmental analysis
  • food quality control
  • diagnostics

Published Papers (2 papers)

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Research

15 pages, 2396 KiB  
Article
Topoisomerase 1 Activity Is Reduced in Response to Thermal Stress in Fruit Flies and in Human HeLa Cells
by Trine Juul-Kristensen, Josephine Geertsen Keller, Kathrine Nygaard Borg, Noriko Y. Hansen, Amalie Foldager, Rasmus Ladegaard, Yi-Ping Ho, Volker Loeschcke and Birgitta R. Knudsen
Biosensors 2023, 13(11), 950; https://0-doi-org.brum.beds.ac.uk/10.3390/bios13110950 - 24 Oct 2023
Viewed by 1252
Abstract
In the modern world with climate changes and increasing pollution, different types of stress are becoming an increasing challenge. Hence, the identification of reliable biomarkers of stress and accessible sensors to measure such biomarkers are attracting increasing attention. In the current study, we [...] Read more.
In the modern world with climate changes and increasing pollution, different types of stress are becoming an increasing challenge. Hence, the identification of reliable biomarkers of stress and accessible sensors to measure such biomarkers are attracting increasing attention. In the current study, we demonstrate that the activity, but not the expression, of the ubiquitous enzyme topoisomerase 1 (TOP1), as measured in crude cell extracts by the REEAD sensor system, is markedly reduced in response to thermal stress in both fruit flies (Drosophila melanogaster) and cultivated human cells. This effect was observed in response to both mild-to-moderate long-term heat stress and more severe short-term heat stress in D. melanogaster. In cultivated HeLa cells a reduced TOP1 activity was observed in response to both cold and heat stress. The reduced TOP1 activity appeared dependent on one or more cellular pathways since the activity of purified TOP1 was unaffected by the utilized stress temperatures. We demonstrate successful quantitative measurement of TOP1 activity using an easily accessible chemiluminescence readout for REEAD pointing towards a sensor system suitable for point-of-care assessment of stress responses based on TOP1 as a biomarker. Full article
(This article belongs to the Special Issue Advances in Amplification Methods for Biosensors (Volume II))
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14 pages, 1789 KiB  
Article
Development of an Electrochemical Sensor for SARS-CoV-2 Detection Based on Loop-Mediated Isothermal Amplification
by Ane Rivas-Macho, Unai Eletxigerra, Ruth Diez-Ahedo, Ángela Barros, Santos Merino, Felipe Goñi-de-Cerio and Garbiñe Olabarria
Biosensors 2023, 13(10), 924; https://0-doi-org.brum.beds.ac.uk/10.3390/bios13100924 - 11 Oct 2023
Cited by 1 | Viewed by 1330
Abstract
The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused more than 6 million deaths all over the world, demonstrating the need for a simple, fast and cost-effective point-of-care (POC) test for the detection of the virus. In this work, [...] Read more.
The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused more than 6 million deaths all over the world, demonstrating the need for a simple, fast and cost-effective point-of-care (POC) test for the detection of the virus. In this work, we developed an electrochemical sensor for SARS-CoV-2 virus detection on clinical samples based on loop-mediated isothermal amplification (LAMP). With the development of this novel sensor, the time of each measurement is significantly reduced by avoiding the DNA extraction step and replacing it with inactivation of the sample by heating it at 95 °C for 10 min. To make the reaction compatible with the sample pre-treatment, an RNase inhibitor was added directly to the premix. The LAMP product was measured in a novel, easy-to-use manufactured sensor containing a custom-made screen-printed carbon electrode. Electrochemical detection was performed with a portable potentiostat, and methylene blue was used as the redox-transducing molecule. The developed sensor achieved a limit of detection of 62 viral copies and was 100% specific for the detection of the SARS-CoV-2 virus. The performance of the electrochemical sensor was validated with nasopharyngeal samples, obtaining a sensibility and specificity of 100% compared to the gold standard RT-PCR method. Full article
(This article belongs to the Special Issue Advances in Amplification Methods for Biosensors (Volume II))
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