Submit to Biosensors Review for Biosensors Propose a Special Issue

Journal Menu

Journal Browser

► Journal Browser

Advances in Magnetic Particle-Based Bioassays

Special Issue Editors
Special Issue Information
Keywords
Published Papers

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 14948

Share This Special Issue

Special Issue Editor

Dr. Carole Chaix

E-Mail Website
Guest Editor

Institut des Sciences Analytiques, UMR 5280 CNRS Université de Lyon, Université Lyon 1, 69100 Villeurbanne, France
Interests: biosensor; bioconjugate; nanotechnology; interface; electrochemical detection; multidetection; methylene blue; ferrocene; DNA; nanomedicine; nanostructuration; diamond; gold; graphene; multi-functionalization
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The special issue aims to highlight the latest experimental approaches and methods using magnetic particles to develop rapid bioassays for point-of-care diagnostics, agri-food safety or environmental quality control. Applications in the field of nanomedicine are also of great interest.

Magnetic beads are commonly used in bioassays thanks to their easy functionalization by specific biomarkers, and their ability to efficiently capture and pre-concentration targets to be detected from biological fluids or complex matrices. Nowadays, these approaches are increasingly integrated into microfluidic, potentially multiplexable, lab-on-a-chip systems, in order to improve the sensitivity/quantification and specificity of the assays, and to reduce the quantities of samples to be analyzed. Recent advances focus on the development of smaller and more efficient magnetic particles well-adapted to microfluidic systems. The latest applications also concern the use of magnetic beads in screening and extraction methods such as aptamer selection, miRNA pre-concentration or exosome isolation.

The scope of the issue includes innovative approaches using functionalised magnetic micro/nanoparticles based on different biological (e.g., enzymes, antibodies, nucleic acids, tissues, organelles, etc.), and (bio)synthetic (e.g., peptides, molecularly imprinted polymers, affimers, oligonucleotides, aptamers, etc.) materials. A wide range of biosensing methods are applicable (e.g., optical, electrochemical, piezoelectric, thermometric, micromechanical, magnetoresistive, etc.). Research dedicated to the development of hybrid or more performant magnetic nanomaterials as well as innovative surface chemistries for biofunctionalization are encouraged. All bioanalytical applications are considered, with a particular interest for bioassays adapted to portable and cost-effective devices. In summary, this issue focuses on innovative technologies and up-to-date methods using magnetic particles which help advance science in the bioanalytical field.

Dr. Carole Chaix
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

magnetic particle
bioassay
biosensing
bioanalysis
point-of-care diagnostics
agri-food
environmental monitoring
rapid test
biofunctionalization
nanoparticle surface chemistry
magnetic extraction

Published Papers (7 papers)

Download All Papers

Research

Jump to: Review

15 pages, 1371 KiB

Open AccessArticle

Patient Stratification for Antibiotic Prescriptions Based on the Bound-Free Phase Detection Immunoassay of C-Reactive Protein in Serum Samples

by Benita Johannsen, Desirée Baumgartner, Michal Karpíšek, David Stejskal, Noémie Boillat-Blanco, José Knüsli, Marcus Panning, Nils Paust, Roland Zengerle and Konstantinos Mitsakakis

Biosensors 2023, 13(12), 1009; https://0-doi-org.brum.beds.ac.uk/10.3390/bios13121009 - 03 Dec 2023

Cited by 1 | Viewed by 1323

Abstract

C-reactive protein is a well-studied host response biomarker, whose diagnostic performance depends on its accurate classification into concentration zones defined by clinical scenario-specific cutoff values. We validated a newly developed, bead-based, bound-free phase detection immunoassay (BFPD-IA) versus a commercial CE-IVD enzyme-linked immunosorbent assay (ELISA) kit and a commercial CE-IVD immunoturbidimetric assay (ITA) kit. The latter was performed on a fully automated DPC Konelab 60i clinical analyzer used in routine diagnosis. We classified 53 samples into concentration zones derived from four different sets of cutoff values that are related to antibiotic prescription scenarios in the case of respiratory tract infections. The agreements between the methods were ELISA/ITA at 87.7%, ELISA/BFPD-IA at 87.3%, and ITA/-BFPD-IA at 93.9%, reaching 98–99% in all cases when considering the calculated relative combined uncertainty of the single measurement of each sample. In a subgroup of 37 samples, which were analyzed for absolute concentration quantification, the scatter plot slopes’ correlations were as follows: ELISA/ITA 1.15, R² = 0.97; BFPD-IA/ELISA 1.12, R² = 0.95; BFPD-IA/ITA 0.95, R² = 0.93. These very good performances and the agreement between BFPD-IA and ITA (routine diagnostic), combined with BFPD-IA’s functional advantages over ITA (and ELISA)—such as quick time to result (~20 min), reduced consumed reagents (only one assay buffer and no washing), few and easy steps, and compatibility with nucleic-acid-amplification instruments—render it a potential approach for a reliable, cost-efficient, evidence-based point-of-care diagnostic test for guiding antibiotic prescriptions. Full article

(This article belongs to the Special Issue Advances in Magnetic Particle-Based Bioassays)

► Show Figures

Figure 1

19 pages, 3947 KiB

Open AccessArticle

Rapid Isolation of Low-Level Carbapenem-Resistant E. coli from Water and Foods Using Glycan-Coated Magnetic Nanoparticles

by Oznur Caliskan-Aydogan, Saad Asadullah Sharief and Evangelyn C. Alocilja

Biosensors 2023, 13(10), 902; https://0-doi-org.brum.beds.ac.uk/10.3390/bios13100902 - 23 Sep 2023

Cited by 1 | Viewed by 1316

Abstract

Carbapenem-resistant Enterobacterales (CRE) are one of the major global issues needing attention. Among them, carbapenemase-producing (CP) E. coli strains are commonly found in clinical and biological samples. Rapid and cost-effective detection of such strains is critical in minimizing their deleterious impact. While promising progress is being made in rapid detection platforms, separation and enrichment of bacteria are required to ensure the detection of low bacterial counts. The current separation methods, such as centrifugation, filtration, electrophoresis, and immunomagnetic separation, are often tedious, expensive, or ineffective for clinical and biological samples. Further, the extraction and concentration of antimicrobial-resistant bacteria (ARB) are not well documented. Thus, this study assessed the applicability of cost-effective glycan-coated magnetic nanoparticles (gMNPs) for simple and rapid extraction of CP E. coli. The study included two resistant (R)strains: Klebsiella pneumoniae carbapenemase (KPC)-producing E. coli (R: KPC) and New Delhi metallo-β-lactamase (NDM)-producing E. coli (R: NDM). A susceptible E. coli (S) strain was used as a control, a reference bacterium. The gMNPs successfully extracted and concentrated E. coli (R) and E. coli (S) at low concentrations from large volumes of buffer solution, water, and food samples. The gMNPs concentrated up to two and five times their initial concentration for E. coli (R) and E. coli (S) in the buffer solution, respectively. In water and food samples, the concentration of E. coli (S) and E. coli (R) were similar and ranged 1–3 times their initial inoculation. A variation in the concentration from different food samples was seen, displaying the impact of food microstructure and natural microflora. The cost-effective and rapid bacterial cell capture by gMNPs was achieved in 15 min, and its successful binding to the bacterial cells in the buffer solution and food matrices was also confirmed using Transmission Electron Microscopy (TEM). These results show promising applications of gMNPs to extract pathogens and ARB from biological samples. Full article

(This article belongs to the Special Issue Advances in Magnetic Particle-Based Bioassays)

► Show Figures

Figure 1

15 pages, 4507 KiB

Open AccessArticle

Post-Vaccination Detection of SARS-CoV-2 Antibody Response with Magnetic Nanoparticle-Based Electrochemical Biosensor System

by Duygu Harmanci, Simge Balaban Hanoglu, Gozde Akkus Kayali, Evrim Durgunlu, Nursima Ucar, Candan Cicek and Suna Timur

Biosensors 2023, 13(9), 851; https://0-doi-org.brum.beds.ac.uk/10.3390/bios13090851 - 26 Aug 2023

Cited by 1 | Viewed by 1083

Abstract

Here, we report magnetic nanoparticle-based biosensor platforms for the rapid detection of SARS-CoV-2 antibody responses in human serum. The use of the proposed system enabled the detection of anti-SARS-CoV-2 spike (S) and nucleocapsid (N) proteins at a concentration of ng/mL in both buffer and real serum samples. In particular, the protocol, which is considered an indicator of innate immunity after vaccination or post-infection, could be useful for the evaluation of antibody response. We included a total of 48 volunteers who either had COVID-19 but were not vaccinated or who had COVID-19 and were vaccinated with CoronoVac or Biontech. Briefly, in this study, which was planned as a cohort, serum samples were examined 3, 6, and 12 months from the time the volunteers’ showed symptoms of COVID-19 with respect to antibody response in the proposed system. Anti-S Ab and anti-N Ab were detected with a limit of detection of 0.98 and 0.89 ng/mL, respectively. These data were confirmed with the corresponding commercial an electrochemiluminescence immunoassay (ECLIA) assays. Compared with ECLIA, more stable data were obtained, especially for samples collected over 6 months. After this period, a drop in the antibody responses was observed. Our findings showed that it could be a useful platform for exploring the dynamics of the immune response, and the proposed system has translational use potential for the clinic. In conclusion, the MNP-based biosensor platform proposed in this study, together with its counterparts in previous studies, is a candidate for determining natural immunity and post-vaccination antibody response, as well as reducing the workload of medical personnel and paving the way for screening studies on vaccine efficacy. Full article

(This article belongs to the Special Issue Advances in Magnetic Particle-Based Bioassays)

► Show Figures

Figure 1

14 pages, 1592 KiB

Open AccessArticle

Serum Autoantibody Biomarkers for Management of Rheumatoid Arthritis Disease

by Esther Sánchez-Tirado, Lourdes Agüí, Marta Sánchez-Paniagua, Araceli González-Cortés, Beatriz López-Ruiz, Paloma Yáñez-Sedeño and José M. Pingarrón

Biosensors 2023, 13(3), 381; https://0-doi-org.brum.beds.ac.uk/10.3390/bios13030381 - 13 Mar 2023

Cited by 1 | Viewed by 1614

Abstract

Rheumatoid arthritis (RA) is a systemic chronic autoimmune inflammatory disease that is characterized by the destruction of bone and production of autoantibodies such as rheumatoid factor (RF) and anticitrullinated protein antibodies (ACPAs). The high prevalence of this disease and the need of affordable tools for its early detection led us to prepare the first electrochemical immunoplatform for the simultaneous determination of four RA biomarkers, the autoantibodies: RF, anti-peptidyl-arginine deiminase enzyme (anti-PAD4), anti-cyclic citrullinated peptide (anti-CCP), and anti-citrullinated vimentin (anti-MCV). Functionalized magnetic beads (MBs) were used to immobilize the specific antigens, and sandwich-type immunoassays were implemented for the amperometric detection of the four autoantibodies, using the horseradish peroxidase (HRP)/H₂O₂/hydroquinone (HQ) system. The immunoplatform was applied to the determination of the biomarkers in human serum of twenty-two patients diagnosed with RA and four healthy individuals, and the results were validated against ELISA tests and the certified values. Full article

(This article belongs to the Special Issue Advances in Magnetic Particle-Based Bioassays)

► Show Figures

Figure 1

17 pages, 3411 KiB

Open AccessArticle

Magnetic Nanoparticle-Based Electrochemical Sensing Platform Using Ferrocene-Labelled Peptide Nucleic Acid for the Early Diagnosis of Colorectal Cancer

by Simge Balaban Hanoglu, Ezgi Man, Duygu Harmanci, Serife Tozan Ruzgar, Serdar Sanli, Nazim Arda Keles, Atakan Ayden, Bilge Guvenc Tuna, Ozgul Duzgun, Omer Faruk Ozkan, Soner Dogan, Faezeh Ghorbanizamani, Hichem Moulahoum, Emine Guler Celik, Serap Evran and Suna Timur

Biosensors 2022, 12(9), 736; https://0-doi-org.brum.beds.ac.uk/10.3390/bios12090736 - 07 Sep 2022

Cited by 13 | Viewed by 3395

Abstract

Diagnostic biomarkers based on epigenetic changes such as DNA methylation are promising tools for early cancer diagnosis. However, there are significant difficulties in directly and specifically detecting methylated DNA regions. Here, we report an electrochemical sensing system based on magnetic nanoparticles that enable a quantitative and selective analysis of the methylated septin9 (mSEPT9) gene, which is considered a diagnostic marker in early stage colorectal cancer (CRC). Methylation levels of SEPT9 in CRC samples were successfully followed by the selective recognition ability of a related peptide nucleic acid (PNA) after hybridization with DNA fragments in human patients’ serums and plasma (n = 10). Moreover, this system was also adapted into a point-of-care (POC) device for a one-step detection platform. The detection of mSEPT9 demonstrated a limit of detection (LOD) value of 0.37% and interference-free measurement in the presence of branched-chain amino acid transaminase 1 (BCAT1) and SRY box transcription factor 21 antisense divergent transcript 1 (SOX21-AS1). The currently proposed functional platform has substantial prospects in translational applications of early CRC detection. Full article

(This article belongs to the Special Issue Advances in Magnetic Particle-Based Bioassays)

► Show Figures

Figure 1

Review

Jump to: Research

23 pages, 3908 KiB

Open AccessReview

A Review of Magnetic Nanoparticle-Based Surface-Enhanced Raman Scattering Substrates for Bioanalysis: Morphology, Function and Detection Application

by Hanbing Huang, Zhuomin Zhang and Gongke Li

Biosensors 2023, 13(1), 30; https://0-doi-org.brum.beds.ac.uk/10.3390/bios13010030 - 27 Dec 2022

Cited by 6 | Viewed by 2260

Abstract

Surface-enhanced Raman scattering (SERS) is a kind of popular non-destructive and water-free interference analytical technology with fast response, excellent sensitivity and specificity to trace biotargets in biological samples. Recently, many researches have focused on the preparation of various magnetic nanoparticle-based SERS substrates for developing efficient bioanalytical methods, which greatly improved the selectivity and accuracy of the proposed SERS bioassays. There has been a rapid increase in the number of reports about magnetic SERS substrates in the past decade, and the number of related papers and citations have exceeded 500 and 2000, respectively. Moreover, most of the papers published since 2009 have been dedicated to analytical applications. In the paper, the recent advances in magnetic nanoparticle-based SERS substrates for bioanalysis were reviewed in detail based on their various morphologies, such as magnetic core–shell nanoparticles, magnetic core–satellite nanoparticles and non-spherical magnetic nanoparticles and their different functions, such as separation and enrichment, recognition and SERS tags. Moreover, the typical application progress on magnetic nanoparticle-based SERS substrates for bioanalysis of amino acids and protein, DNA and RNA sequences, cancer cells and related tumor biomarkers, etc., was summarized and introduced. Finally, the future trends and prospective for SERS bioanalysis by magnetic nanoparticle-based substrates were proposed based on the systematical study of typical and latest references. It is expected that this review would provide useful information and clues for the researchers with interest in SERS bioanalysis. Full article

(This article belongs to the Special Issue Advances in Magnetic Particle-Based Bioassays)

► Show Figures

Figure 1

36 pages, 7343 KiB

Open AccessReview

Overview on the Design of Magnetically Assisted Electrochemical Biosensors

by Yong Chang, Yanyan Wang, Jingyi Zhang, Yuejiao Xing, Gang Li, Dehua Deng and Lin Liu

Biosensors 2022, 12(11), 954; https://0-doi-org.brum.beds.ac.uk/10.3390/bios12110954 - 01 Nov 2022

Cited by 7 | Viewed by 2693

Abstract

Electrochemical biosensors generally require the immobilization of recognition elements or capture probes on the electrode surface. This may limit their practical applications due to the complex operation procedure and low repeatability and stability. Magnetically assisted biosensors show remarkable advantages in separation and pre-concentration of targets from complex biological samples. More importantly, magnetically assisted sensing systems show high throughput since the magnetic materials can be produced and preserved on a large scale. In this work, we summarized the design of electrochemical biosensors involving magnetic materials as the platforms for recognition reaction and target conversion. The recognition reactions usually include antigen–antibody, DNA hybridization, and aptamer–target interactions. By conjugating an electroactive probe to biomolecules attached to magnetic materials, the complexes can be accumulated near to an electrode surface with the aid of external magnet field, producing an easily measurable redox current. The redox current can be further enhanced by enzymes, nanomaterials, DNA assemblies, and thermal-cycle or isothermal amplification. In magnetically assisted assays, the magnetic substrates are removed by a magnet after the target conversion, and the signal can be monitored through stimuli–response release of signal reporters, enzymatic production of electroactive species, or target-induced generation of messenger DNA. Full article

(This article belongs to the Special Issue Advances in Magnetic Particle-Based Bioassays)

► Show Figures

Figure 1

Journal Menu

Journal Browser

Advances in Magnetic Particle-Based Bioassays

Share This Special Issue

Special Issue Editor

Special Issue Information

Keywords

Published Papers (7 papers)

Research

Review

Further Information

Guidelines

MDPI Initiatives

Follow MDPI