Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
3.9
4.2
Journals
Chemosensors
Special Issues
Microfluidic Biosensing Platform
share announcement

Microfluidic Biosensing Platform

A special issue of Chemosensors (ISSN 2227-9040). This special issue belongs to the section "Applied Chemical Sensors".

Deadline for manuscript submissions: closed (20 May 2022) | Viewed by 33185

Special Issue Editor

Dr. Patricia Khashayar

E-Mail Website1 Website2
Guest Editor
Center for Microsystems Technology, Imec and Ghent University, 9050 Ghent, Belgium
Interests: osteoporosis; biosensors; microfluidic; cervical cancer; proteomics; immunosensors; in-vitro diagnostics; point-of-care; diagnostics; medical devices; nanotechnology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The integration of microfluidic platforms and biosensing technologies is a rapidly developing field with major applications towards in-vitro diagnostic devices. This is of the utmost importance in healthcare, where early and accurate diagnosis, especially as point-of-care (PoC), without expert intervention, can affect the course of the treatment. In other words, the combination of these technologies at the bedside or even at home, away from centralized care facilities, can result in equality in access to healthcare even in remote areas, resource-limited settings, or under infrastructure shortfalls.

Replacing single-analyte measurements with multiplexing microsystems also allows for the simultaneous measurement of several biological analytes in a controllable environment with a reduced amount of analytes, in a shorter time and at a lower price.

In brief, these technologies provide a patient-centered care approach, making personalized screening, diagnosis, and prognostic assessments possible at decreased turnaround time, reduced cost, and real-time response.

This Special Issue is a research forum covering recent development and innovations in microfluidic-based biosensors and in-vitro diagnostics with various applications, as well as new fabrication and integration technologies relevant to biosensing and actuation. Researchers working in a wide range of disciplines are invited to contribute research papers, short communications, or reviews that include, but are not limited to, the following:

  • Multiplexed bioassays/sensor arrays, including electrochemical, optical, magnetic, and other transduction types;
  • Novel microfluidic-based biosensing concepts, mechanisms, and detection principles;
  • Lab-on-a-chip and other biochips and microarray systems;
  • Low-cost diagnostics
  • Wearable biosensors
  • Advances in instrumentation (including 3D printing, scaffold-based biomimetic systems, Micro total analysis systems (μTAS))

Dr. Patricia Khashayar
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Chemosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Biosensors
  • Lab-on-chip
  • Microfluidics
  • Nanotechnology
  • In-vitro diagnostics
  • Point of care
  • Diagnostics

Published Papers (9 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

14 pages, 5091 KiB  
Article
A 4 × 4 Array of Complementary Split-Ring Resonators for Label-Free Dielectric Spectroscopy
by Matko Martinic, Tomislav Markovic, Adrijan Baric and Bart Nauwelaers
Chemosensors 2021, 9(12), 348; https://0-doi-org.brum.beds.ac.uk/10.3390/chemosensors9120348 - 08 Dec 2021
Cited by 6 | Viewed by 2584
Abstract
In this study, complementary split-ring resonator (CSRR) metamaterial structures are proposed for label-free dielectric spectroscopy of liquids in microplates. This novel combination of an array of sensors and microplates is readily scalable and thus offers a great potential for non-invasive, rapid, and label-free [...] Read more.
In this study, complementary split-ring resonator (CSRR) metamaterial structures are proposed for label-free dielectric spectroscopy of liquids in microplates. This novel combination of an array of sensors and microplates is readily scalable and thus offers a great potential for non-invasive, rapid, and label-free dielectric spectroscopy of liquids in large microplate arrays. The proposed array of sensors on a printed circuit board consists of a microstrip line coupled to four CSRRs in cascade with resonant frequencies ranging from 7 to 10 GHz, spaced around 1 GHz. The microwells were manufactured and bonded to the CSRR using polydimethylsiloxane, whose resonant frequency is dependent on a complex relative permittivity of the liquid loaded in the microwell. The individual microstrip lines with CSRRs were interconnected to the measurement equipment using two electronically controllable microwave switches, which enables microwave measurements of the 4 × 4 CSRR array using only a two-port measurement system. The 4 × 4 microwell sensor arrays were calibrated and evaluated using water-ethanol mixtures with different ethanol concentrations. The proposed measurement setup offers comparable results to ones obtained using a dielectric probe, confirming the potential of the planar sensor array for large-scale microplate experiments. Full article
(This article belongs to the Special Issue Microfluidic Biosensing Platform)
►▼ Show Figures

Figure 1

12 pages, 4054 KiB  
Article
Off-Grid Electrical Cell Lysis Microfluidic Device Utilizing Thermoelectricity and Thermal Radiation
by Duong-Duy Duong and Nae-Yoon Lee
Chemosensors 2021, 9(10), 292; https://0-doi-org.brum.beds.ac.uk/10.3390/chemosensors9100292 - 14 Oct 2021
Cited by 1 | Viewed by 1832
Abstract
Microfluidic devices have enormous potential and a wide range of applications. However, most applications end up as chip-in-a-lab systems because of power source constraints. This work focuses on reducing the reliance on the power network and expanding on the concept of a lab-on-a-chip [...] Read more.
Microfluidic devices have enormous potential and a wide range of applications. However, most applications end up as chip-in-a-lab systems because of power source constraints. This work focuses on reducing the reliance on the power network and expanding on the concept of a lab-on-a-chip for microfluidic devices. A cellulose-based radiator to reflect infrared (IR) radiation with wavelengths within the atmospheric window (8–13 µm) into outer space was fabricated. This process lowered the temperature inside the insulated environment. The difference in temperature was used to power a thermoelectric generator (TEG) and generate an electric current. This electric current was run through a DC-DC transformer to increase the voltage before being used to perform electrical cell lysis with a microfluidic device. This experimental setup successfully achieved 90% and 50% cell lysis efficiencies in ideal conditions and in field tests, respectively. This work demonstrated the possibility of utilizing the unique characteristics of a microfluidic device to perform an energy-intensive assay with minimal energy generated from a TEG and no initial power input for the system. The TEG system also required less maintenance than solar, wind, or hydroelectricity. The IR radiation process naturally allows for more dynamic working conditions for the entire system. Full article
(This article belongs to the Special Issue Microfluidic Biosensing Platform)
►▼ Show Figures

Graphical abstract

15 pages, 3379 KiB  
Article
Electrochemical Performance of Lithographically-Defined Micro-Electrodes for Integration and Device Applications
by Zohreh Hirbodvash, Mohamed S. E. Houache, Oleksiy Krupin, Maryam Khodami, Howard Northfield, Anthony Olivieri, Elena A. Baranova and Pierre Berini
Chemosensors 2021, 9(10), 277; https://0-doi-org.brum.beds.ac.uk/10.3390/chemosensors9100277 - 28 Sep 2021
Cited by 5 | Viewed by 2908
Abstract
Small; lithographically-defined and closely-spaced metallic features of dimensions and separation in the micrometer range are of strong interest as working and counter electrodes in compact electrochemical sensing devices. Such micro-electrode systems can be integrated with microfluidics and optical biosensors, such as surface plasmon [...] Read more.
Small; lithographically-defined and closely-spaced metallic features of dimensions and separation in the micrometer range are of strong interest as working and counter electrodes in compact electrochemical sensing devices. Such micro-electrode systems can be integrated with microfluidics and optical biosensors, such as surface plasmon waveguide biosensors, to enable multi-modal sensing strategies. We investigate lithographically-defined gold and platinum micro-electrodes experimentally, via cyclic voltammetry (CV) measurements obtained at various scan rates and concentrations of potassium ferricyanide as the redox species, in potassium nitrate as the supporting electrolyte. The magnitude of the double-layer capacitance is estimated using the voltammograms. Concentration curves for potassium ferricyanide are extracted from our CV measurements as a function of scan rate, and could be used as calibration curves from which an unknown concentration of potassium ferricyanide in the range of 0.5–5 mM can be determined. A blind test was done to confirm the validity of the calibration curve. The diffusion coefficient of potassium ferricyanide is also extracted from our CV measurements by fitting to the Randles–Sevcik equation (D = 4.18 × 10−10 m2/s). Our CV measurements were compared with measurements obtained using macroscopic commercial electrodes, yielding good agreement and verifying that the shape of our CV curves do not depend on micro-electrode geometry (only on area). We also compare our CV measurements with theoretical curves computed using the Butler–Volmer equation, achieving essentially perfect agreement while extracting the rate constant at zero potential for our redox species (ko = 10−6 m/s). Finally, we demonstrate the importance of burn-in to stabilize electrodes from the effects of electromigration and grain reorganization before use in CV measurements, by comparing with results obtained with as-deposited electrodes. Burn-in (or equivalently, annealing) of lithographic microelectrodes before use is of general importance to electrochemical sensing devices Full article
(This article belongs to the Special Issue Microfluidic Biosensing Platform)
►▼ Show Figures

Figure 1

12 pages, 4164 KiB  
Article
Complementary Split-Ring Resonator for Microwave Heating of µL Volumes in Microwells in Continuous Microfluidics
by Tomislav Markovic, Gertjan Maenhout, Matko Martinic and Bart Nauwelaers
Chemosensors 2021, 9(7), 184; https://0-doi-org.brum.beds.ac.uk/10.3390/chemosensors9070184 - 17 Jul 2021
Cited by 3 | Viewed by 2512
Abstract
This work presents the design and evaluation of a planar device for microwave heating of liquids in continuous microfluidics (CMF) made in polydimethylsiloxane (PDMS). It deals with volumes in the µL range, which are of high interest and relevance to biologists and chemists. [...] Read more.
This work presents the design and evaluation of a planar device for microwave heating of liquids in continuous microfluidics (CMF) made in polydimethylsiloxane (PDMS). It deals with volumes in the µL range, which are of high interest and relevance to biologists and chemists. The planar heater in this work is conceived around a complementary split-ring resonator (CSRR) topology that offers a desired electric field direction to—and interaction with—liquids in a microwell. The designed device on a 0.25 mm thick Rogers RO4350B substrate operates at around 2.5 GHz, while a CMF channel and a 2.45 µL microwell are manufactured in PDMS using the casting process. The evaluation of the performance of the designed heater is conducted using a fluorescent dye, Rhodamine B, dissolved in deionized water. Heating measurements are carried out using 1 W of power and the designed device achieves a temperature of 47 °C on a sample volume of 2.45 µL after 20 s of heating. Based on the achieved results, the CSRR topology has a large potential in microwave heating, in addition to the already demonstrated potential in microwave dielectric sensing, all proving the multifunctionality and reusability of single planar microwave-microfluidic devices. Full article
(This article belongs to the Special Issue Microfluidic Biosensing Platform)
►▼ Show Figures

Figure 1

17 pages, 2035 KiB  
Article
Compact Miniaturized Bioluminescence Sensor Based on Continuous Air-Segmented Flow for Real-Time Monitoring: Application to Bile Salt Hydrolase (BSH) Activity and ATP Detection in Biological Fluids
by Aldo Roda, Pierpaolo Greco, Patrizia Simoni, Valentina Marassi, Giada Moroni, Antimo Gioiello and Barbara Roda
Chemosensors 2021, 9(6), 122; https://0-doi-org.brum.beds.ac.uk/10.3390/chemosensors9060122 - 25 May 2021
Cited by 2 | Viewed by 2570
Abstract
A simple and versatile continuous air-segmented flow sensor using immobilized luciferase was designed as a general miniaturized platform based on sensitive biochemiluminescence detection. The device uses miniaturized microperistaltic pumps to deliver flows and compact sensitive light imaging detectors based on BI-CMOS (smartphone camera) [...] Read more.
A simple and versatile continuous air-segmented flow sensor using immobilized luciferase was designed as a general miniaturized platform based on sensitive biochemiluminescence detection. The device uses miniaturized microperistaltic pumps to deliver flows and compact sensitive light imaging detectors based on BI-CMOS (smartphone camera) or CCD technology. The low-cost components and power supply make it suitable as out-lab device at point of need to monitor kinetic-related processes or ex vivo dynamic events. A nylon6 flat spiral carrying immobilized luciferase was placed in front of the detector in lensless mode using a fiber optic tapered faceplate. ATP was measured in samples collected by microdialysis from rat brain with detecting levels as low as 0.4 fmoles. The same immobilized luciferase was also used for the evaluation of bile salt hydrolase (BSH) activity in intestinal microbiota. An aminoluciferin was conjugatated with chenodeoxycholic acid forming the amide derivative aLuc-CDCA. The hydrolysis of the aLuc-CDCA probe by BSH releases free uncaged aminoluciferin which is the active substrate for luciferase leading to light emission. This method can detect as low as 0.5 mM of aLuc-CDCA, so it can be used on real faecal human samples to study BSH activity and its modulation by diseases and drugs. Full article
(This article belongs to the Special Issue Microfluidic Biosensing Platform)
►▼ Show Figures

Figure 1

Review

Jump to: Research

17 pages, 1505 KiB  
Review
Advances in Nucleic Acid Amplification-Based Microfluidic Devices for Clinical Microbial Detection
by Thi Ngoc Diep Trinh and Nae Yoon Lee
Chemosensors 2022, 10(4), 123; https://0-doi-org.brum.beds.ac.uk/10.3390/chemosensors10040123 - 25 Mar 2022
Cited by 11 | Viewed by 3692
Abstract
Accurate and timely detection of infectious pathogens is urgently needed for disease treatment and control of possible outbreaks worldwide. Conventional methods for pathogen detection are usually time-consuming and labor-intensive. Novel strategies for the identification of pathogenic nucleic acids are necessary for practical application. [...] Read more.
Accurate and timely detection of infectious pathogens is urgently needed for disease treatment and control of possible outbreaks worldwide. Conventional methods for pathogen detection are usually time-consuming and labor-intensive. Novel strategies for the identification of pathogenic nucleic acids are necessary for practical application. The advent of microfluidic technology and microfluidic devices has offered advanced and miniaturized tools to rapidly screen microorganisms, improving many drawbacks of conventional nucleic acid amplification-based methods. In this review, we summarize advances in the microfluidic approach to detect pathogens based on nucleic acid amplification. We survey microfluidic platforms performing two major types of nucleic acid amplification strategies, namely, polymerase chain reaction (PCR) and isothermal nucleic acid amplification. We also provide an overview of nucleic acid amplification-based platforms including studies and commercialized products for SARS-CoV-2 detection. Technologically, we focus on the design of the microfluidic devices, the selected methods for sample preparation, nucleic acid amplification techniques, and endpoint analysis. We also compare features such as analysis time, sensitivity, and specificity of different platforms. The first section of the review discusses methods used in microfluidic devices for upstream clinical sample preparation. The second section covers the design, operation, and applications of PCR-based microfluidic devices. The third section reviews two common types of isothermal nucleic acid amplification methods (loop-mediated isothermal amplification and recombinase polymerase amplification) performed in microfluidic systems. The fourth section introduces microfluidic applications for nucleic acid amplification-based detection of SARS-CoV-2. Finally, the review concludes with the importance of full integration and quantitative analysis for clinical microbial identification. Full article
(This article belongs to the Special Issue Microfluidic Biosensing Platform)
►▼ Show Figures

Figure 1

20 pages, 4027 KiB  
Review
CRISPR-Powered Microfluidics in Diagnostics: A Review of Main Applications
by Mostafa Azimzadeh, Marziyeh Mousazadeh, Atieh Jahangiri-Manesh, Pouria Khashayar and Patricia Khashayar
Chemosensors 2022, 10(1), 3; https://0-doi-org.brum.beds.ac.uk/10.3390/chemosensors10010003 - 24 Dec 2021
Cited by 13 | Viewed by 4207
Abstract
In the past few years, the CRISPR (clustered regularly interspaced short palindromic repeats) applications in medicine and molecular biology have broadened. CRISPR has also been integrated with microfluidic-based biosensors to enhance the sensitivity and selectivity of medical diagnosis due to its great potentials. [...] Read more.
In the past few years, the CRISPR (clustered regularly interspaced short palindromic repeats) applications in medicine and molecular biology have broadened. CRISPR has also been integrated with microfluidic-based biosensors to enhance the sensitivity and selectivity of medical diagnosis due to its great potentials. The CRISPR-powered microfluidics can help quantify DNAs and RNAs for different diseases such as cancer, and viral or bacterial diseases among others. Here in this review, we discussed the main applications of such tools along with their advantages and limitations. Full article
(This article belongs to the Special Issue Microfluidic Biosensing Platform)
►▼ Show Figures

Graphical abstract

18 pages, 4625 KiB  
Review
Recent Microfluidic Innovations for Sperm Sorting
by Maedeh Khodamoradi, Saeed Rafizadeh Tafti, Seyed Ali Mousavi Shaegh, Behrouz Aflatoonian, Mostafa Azimzadeh and Patricia Khashayar
Chemosensors 2021, 9(6), 126; https://0-doi-org.brum.beds.ac.uk/10.3390/chemosensors9060126 - 01 Jun 2021
Cited by 14 | Viewed by 6364
Abstract
Sperm selection is a clinical need for guided fertilization in men with low-quality semen. In this regard, microfluidics can provide an enabling platform for the precise manipulation and separation of high-quality sperm cells through applying various stimuli, including chemical agents, mechanical forces, and [...] Read more.
Sperm selection is a clinical need for guided fertilization in men with low-quality semen. In this regard, microfluidics can provide an enabling platform for the precise manipulation and separation of high-quality sperm cells through applying various stimuli, including chemical agents, mechanical forces, and thermal gradients. In addition, microfluidic platforms can help to guide sperms and oocytes for controlled in vitro fertilization or sperm sorting using both passive and active methods. Herein, we present a detailed review of the use of various microfluidic methods for sorting and categorizing sperms for different applications. The advantages and disadvantages of each method are further discussed and future perspectives in the field are given. Full article
(This article belongs to the Special Issue Microfluidic Biosensing Platform)
►▼ Show Figures

Figure 1

19 pages, 5198 KiB  
Review
Recent Development in Nanomaterial-Based Electrochemical Sensors for Cholesterol Detection
by Hemraj Mahipati Yadav, Jong-Deok Park, Hyeong-Cheol Kang and Jae-Joon Lee
Chemosensors 2021, 9(5), 98; https://0-doi-org.brum.beds.ac.uk/10.3390/chemosensors9050098 - 29 Apr 2021
Cited by 25 | Viewed by 4694
Abstract
Functional nanomaterials have attracted significant attention in a variety of research fields (in particular, in the healthcare system) because of the easily controllable morphology, their high chemical and environmental stability, biocompatibility, and unique optoelectronic and sensing properties. The sensing properties of nanomaterials can [...] Read more.
Functional nanomaterials have attracted significant attention in a variety of research fields (in particular, in the healthcare system) because of the easily controllable morphology, their high chemical and environmental stability, biocompatibility, and unique optoelectronic and sensing properties. The sensing properties of nanomaterials can be used to detect biomolecules such as cholesterol. Over the past few decades, remarkable progress has been made in the production of cholesterol biosensors that contain nanomaterials as the key component. In this article, various nanomaterials for the electrochemical sensing of cholesterol were reviewed. Cholesterol biosensors are recognized tools in the clinical diagnosis of cardiovascular diseases (CVDs). The function of nanomaterials in cholesterol biosensors were thoroughly discussed. In this study, different pathways for the sensing of cholesterol with functional nanomaterials were investigated. Full article
(This article belongs to the Special Issue Microfluidic Biosensing Platform)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-9
Back to TopTop