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Molecules
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Fluorescent Probes for Biological Applications
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Fluorescent Probes for Biological Applications

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Bioorganic Chemistry".

Deadline for manuscript submissions: closed (30 September 2023) | Viewed by 3581

Special Issue Editors

Dr. Siddharth S. Matikonda

E-Mail Website
Guest Editor
Centre for Cancer Research, National Cancer Institute (NIH), Building 376, Room 218B, Frederick, MD 21702, USA
Interests: fluorescent dyes; antibody–fluorophore conjugates; photoconverting dyes; antibody–drug conjugates; super-resolution microscopy; photoacoustic imaging; in vivo imaging; SWIR imaging; bio-orthogonal chemistry; click reactions
Prof. Dr. Ivo Piantanida

E-Mail Website
Guest Editor
Laboratory for Biomolecular Interactions and Spectroscopy, Head Division of Organic Chemistry and Biochemistry, Rudjer Boskovic Institute, 10000 Zagreb, Croatia
Interests: supramolecular and medicinal chemistry; design and synthesis of dyes; DNA/RNA recognition; small molecule/protein interactions; fluorescence spectroscopy and microscopy; circular dichroism; microcalorimetry methods (ITC; DSC); development of Raman probes for biomacromolecules
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Fluorescent probes have revolutionized the landscape of imaging in chemical biology. They are characterized as molecules that absorb light of a specific wavelength and typically emit radiation of a longer wavelength. Fluorescent probes have been pioneered for applications in molecular biology, medical research, and other fields. The enhanced sensitivity and spatiotemporal resolution unique to this modality have made fluorescence imaging the apparent choice for various in vitro and in vivo applications.

This Special Issue focuses on the biological applications in fluorescence probes, such as bioanalytical, biological, optical imaging, and diagnostic and therapeutic applications. Review articles by experts in the field will also be welcome.

Dr. Siddharth S. Matikonda
Prof. Dr. Ivo Piantanida
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fluorescent probes
  • cell imaging
  • bioimaging tools
  • biological applications
  • biosensing
  • in vivo imaging
  • multicolor imaging
  • single molecule localization microscopy
  • ratiometric imaging

Published Papers (2 papers)

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Research

17 pages, 4618 KiB  
Article
Branched Linkers for Site-Specific Fluorescent Labeling of Antibodies
by Ksenia A. Sapozhnikova, Evgeny L. Gulyak, Vsevolod A. Misyurin, Maria A. Simonova, Ekaterina V. Ryabukhina, Anastasiya V. Alexeeva, Nataliya A. Tikhonova, Natalia A. Lyzhko, Galina P. Popova, Andrey V. Misyurin, Alexey V. Ustinov, Vladimir A. Korshun, Vera A. Alferova, Dmitry Yu. Ryazantsev and Vladimir A. Brylev
Molecules 2023, 28(1), 425; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules28010425 - 03 Jan 2023
Cited by 3 | Viewed by 1943
Abstract
Fluorescent antibodies have proved to be an invaluable tool for molecular biology and diagnostics. They are routinely produced by modification of lysine residues, which leads to high heterogeneity. As such, their affinity may be compromised if the antigen-binding site is affected, the probability [...] Read more.
Fluorescent antibodies have proved to be an invaluable tool for molecular biology and diagnostics. They are routinely produced by modification of lysine residues, which leads to high heterogeneity. As such, their affinity may be compromised if the antigen-binding site is affected, the probability of which increases along with the degree of labeling. In this work, we propose a methodology for the synthesis of site-specific antibody-dye conjugates with a high degree of labeling. To this end, we synthesized two oxyamine-based branched triazide linkers and coupled them with a periodate-oxidized anti-PRAME antibody 6H8; two oxyamine-based linear monoazide linkers of similar structure were used as controls. The azide-labeled antibodies were subsequently conjugated with fluorescent dyes via SPAAC, a copper-free click reaction. Compared to their counterparts made with linear linkers, the branched conjugates possessed a higher degree of labeling. The utility of the methodology was demonstrated in the detection of the PRAME protein on the surface of the cell by flow cytometry. Full article
(This article belongs to the Special Issue Fluorescent Probes for Biological Applications)
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13 pages, 5423 KiB  
Article
New Constructed EEM Spectra Combined with N-PLS Analysis Approach as an Effective Way to Determine Multiple Target Compounds in Complex Samples
by Zeying Li, Na Feng, Xinkang Li, Yuan Lin, Xiangzhi Zhang and Baoqiong Li
Molecules 2022, 27(23), 8378; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27238378 - 01 Dec 2022
Viewed by 1118
Abstract
Excitation–emission matrix (EEM) fluorescence spectroscopy has been applied to many fields. In this study, a simple method was proposed to obtain the new constructed three-dimensional (3D) EEM spectra based on the original EEM spectra. Then, the application of the N-PLS method to the [...] Read more.
Excitation–emission matrix (EEM) fluorescence spectroscopy has been applied to many fields. In this study, a simple method was proposed to obtain the new constructed three-dimensional (3D) EEM spectra based on the original EEM spectra. Then, the application of the N-PLS method to the new constructed 3D EEM spectra was proposed to quantify target compounds in two complex data sets. The quantitative models were established on external sample sets and validated using statistical parameters. For validation purposes, the obtained results were compared with those obtained by applying the N-PLS method to the original EEM spectra and applying the PLS method to the extracted maximum spectra in the concatenated mode. The comparison of the results demonstrated that, given the advantages of less useless information and a high calculating speed of the new constructed 3D EEM spectra, N-PLS on the new constructed 3D EEM spectra obtained better quantitative analysis results with a correlation coefficient of prediction above 0.9906 and recovery values in the range of 85.6–95.6%. Therefore, one can conclude that the N-PLS method combined with the new constructed 3D EEM spectra is expected to be broadened as an alternative strategy for the simultaneous determination of multiple target compounds. Full article
(This article belongs to the Special Issue Fluorescent Probes for Biological Applications)
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