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Plants
Special Issues
Micropropagation, Acclimatization and Genebanking of Horticultural Crops
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Micropropagation, Acclimatization and Genebanking of Horticultural Crops

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Molecular Biology".

Deadline for manuscript submissions: closed (10 February 2024) | Viewed by 8082

Special Issue Editors

Dr. Amir Khoddamzadeh

E-Mail Website
Guest Editor
Department of Earth and Environment, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA
Interests: in vitro micropropagation; genebanking; cryopreservation; endangered and rare plants; optical sensors; sustainable and conservation horticulture
Prof. Dr. Wagner Vendrame

E-Mail Website
Guest Editor
Environmental Horticulture Department, Institute of Food and Agricultural Sciences, University of Florida, 2550 Hull Rd., Gainesville, FL 32611-0670, USA
Interests: micropropagation; cryopreservation; ornamental plants; medicinal plants; horticultural crops; space biology; temporary immersion bioreactors
Special Issues, Collections and Topics in MDPI journals
Dr. Uma Rani Sinniah

E-Mail Website
Guest Editor
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
Interests: seed science and technology; plant physiology; plant tissue culture; cryopreservation; agronomy; medicinal and aromatic plants; agriculture biotechnology; sustainable agriculture

Special Issue Information

Dear Colleagues,

In vitro propagation (or tissue culture) is an important process in plant propagation. After explant induction and somatic embryo/callus production, plant materials can be placed in a suitable medium to become seedlings or encapsulated and transformed into artificial/synthetic (syn) seeds for short- or medium-term preservation or delivery. Due to seedling mortality during the introduction and reintroduction stages, acclimatization, which is the process of transferring plants from an aseptic artificial environment to the outside world, is a vital final step of in vitro micropropagation. The encapsulation process has advantages over traditional micropropagation that can result in the need for excessive subculturing, unpredictable mutations, larger space requirements, and higher labor needs, which are costly. Synthetic seeds containing propagules (plants’ reproducible organs) such as nodes, shoots, callus, somatic embryos, and protocorms are contained in a gelatinous matrix. The use of axillary buds and shoot tips derived in vitro enables the acquisition of inexpensive, easily obtained propagules for storage. To date, cryopreservation is the only vital methods for conserving plant germplasm and living cells in liquid nitrogen. In the past two decades, cryopreservation has been widely used as an alternative method for plant germplasm storage. We welcome both critical review articles and original research articles focusing chiefly, but not exclusively, on topics related to the following keywords.

Dr. Amir Khoddamzadeh
Prof. Dr. Wagner Vendrame
Dr. Uma Rani Sinniah
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • in vitro culture
  • micropropagation
  • horticultural crops
  • tissue culture
  • genebanking
  • cryopreser-vation
  • synthetic seed (artificial seed)
  • seed germination
  • bioreactor
  • ornamentals
  • tropical plants
  • subtropical plants
  • acclimatization
  • medicinal plants
  • ornamental plants
  • flowers
  • fruit trees
  • encapsulation
  • embryo rescue
  • plant growth regulators
  • germplasm conservation
  • botanic gardens

Published Papers (2 papers)

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Research

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18 pages, 4832 KiB  
Article
IPPRAS Cryobank for the Conservation of Orthodox Seeds of Rare, Endangered, Medicinal, and Ornamental Plant Species—Current Research
by Olga Sergeevna Nits, Mariya Vladimirovna Sementsova, Ekaterina Sergeevna Osipova, Dmitry Viktorovich Tereshonok and Evgeny Aleksandrovich Gladkov
Plants 2024, 13(10), 1354; https://0-doi-org.brum.beds.ac.uk/10.3390/plants13101354 - 14 May 2024
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Abstract
Plant cryobanks play a significant role in modern science and breeding. They contribute to the recovery of lost species, the emergence of new plant varieties, and help preserve and explore the diversity of the plant world. The IPPRAS Cryobank collection is constantly supplemented [...] Read more.
Plant cryobanks play a significant role in modern science and breeding. They contribute to the recovery of lost species, the emergence of new plant varieties, and help preserve and explore the diversity of the plant world. The IPPRAS Cryobank collection is constantly supplemented with new samples, while, at the same time, the stored samples are being monitored. In order to test seed germination, seeds of Allium and Veratrum species were thawed. Rare Allium species seeds, such as A. nutans, A. schoenoprasum, and A. victorialis were stored in liquid nitrogen for 17, 19, and 30 years, respectively. Long-term cryopreservation decreased germination rates for A. nutans from 96.55 to 50.00%, for A. schoenoprasum from 72.00 to 62.75%, and for A. victorialis from 90.00 to 83.05%. Seeds of a rare medicinal species, Veratrum lobelianum, were stored in liquid nitrogen for 18 years; the seed germination rate during this storage period has been significantly decreased from 75.00 to 14.81%. V. nigrum seeds were also collected and frozen in liquid nitrogen for 3 days. Short-term cryopreservation did not result in a statistically significant change in germination rates (from 79.71 to 82.69%). The seeds of an endangered ornamental species, Cypripedium calceolus, were collected and kept frozen for 3 days. After cryopreservation, the seeds were planted on three different media, as follows: ½ MS, MS with 10% coconut milk, and BM1. On ½ MS medium, 24.98% seeds formed protocorms, while on MS medium with 10% coconut milk, this number was 10.02%, and on BM1 medium, it was 15.02%, respectively; however, after 2.5 months, all of the protocorms died. Thus, it appears that the existing protocol for seed cryopreservation of C. calceolus needs further improvement. The size, weight, and free water content (WC) of six previously cryopreserved Stipa species and three Allium species were measured. For all the Allium and Stipa species studied, we found no correlation between seed size, WC, and cryotolerance. We also found no correlation between the life form, which reflects the water requirement of the species, and cryotolerance. Full article
(This article belongs to the Special Issue Micropropagation, Acclimatization and Genebanking of Horticultural Crops)
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Review

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32 pages, 2319 KiB  
Review
Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review
by Potshangbam Nongdam, David G. Beleski, Leimapokpam Tikendra, Abhijit Dey, Vanlalrinchhani Varte, Soumaya EL Merzougui, Vania M. Pereira, Patricia R. Barros and Wagner A. Vendrame
Plants 2023, 12(5), 1136; https://0-doi-org.brum.beds.ac.uk/10.3390/plants12051136 - 2 Mar 2023
Cited by 8 | Viewed by 6220
Abstract
Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive [...] Read more.
Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive unregulated commercial collection and mass habitat destruction makes orchid conservation measures an extreme priority. Conventional propagation methods cannot produce adequate number of orchids, which would meet the requirement of these ornamental plants for commercial and conservational purposes. In vitro orchid propagation using semi-solid media offers an outstanding prospect of rapidly producing quality plants on a large scale. However, the semi-solid (SS) system has shortcomings with low multiplication rates and high production costs. Orchid micropropagation using a temporary immersion system (TIS) overcomes the limitations of the SS system by reducing production costs and making scaleup and full automation possible for mass plant production. The current review highlights different aspects of in vitro orchid propagation using SS and TIS and their benefits and drawbacks on rapid plant generation. Full article
(This article belongs to the Special Issue Micropropagation, Acclimatization and Genebanking of Horticultural Crops)
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