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Separations
Special Issues
Chemical and Biological Technology Applied in Natural Food Products Analysis
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Chemical and Biological Technology Applied in Natural Food Products Analysis

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Analysis of Food and Beverages".

Deadline for manuscript submissions: closed (20 February 2023) | Viewed by 4471

Special Issue Editors

Dr. Sónia Soares

E-Mail Website
Guest Editor
REQUIMTE - Instituto Superior de Engenharia do Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072 Porto, Portugal
Interests: food chemistry; analytical chemistry; food quality and authenticity
Dr. Olga Viegas

E-Mail Website
Guest Editor
REQUIMTE - Laboratório de Bromatologia e Hidrologia, Departamento de Ciências Químicas, Faculdade de Farmácia da Universidade do Porto, 4099-313 Porto, Portugal
Interests: food safety and nutritional toxicology; sample preparation and chromatography
Special Issues, Collections and Topics in MDPI journals
Dr. Francisca Rodrigues

E-Mail Website1 Website2
Guest Editor
REQUIMTE/LAQV—Superior Institute of Engineering of Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072 Porto, Portugal
Interests: natural extracts; bioactive compounds; antioxidants; in vitro assays; in vivo cosmetic assays; pharmaceutics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Natural food products are increasingly sought by consumers due to their concern for consuming “healthy” products. The selection of these is usually based on the relation between natural products and beneficial health properties, as well as new knowledge regarding the impacts of production on the environment. Therefore, the evaluation of these products is extremely important, aiming to explore their composition, properties, and possible risks to consumers, the scientific community, and industry. There are several chemical and biological analyses which are normally performed to characterize and determine the safety of food products, including molecular, chromatographic, and spectroscopic methodologies.

The aim of this Special Issue is to present new advances on different methods of analysis and present new approaches used to evaluate natural food products, contributing to their valorization. Papers which have assessed the in vitro and in vivo safety and efficacy of natural products are especially welcomed for submission.

Dr. Sónia Soares
Dr. Olga Viegas
Dr. Francisca Rodrigues
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • natural food products
  • chemical composition
  • biological properties
  • food safety
  • risk assessment
  • food quality
  • chromatography techniques
  • spectroscopic methods

Published Papers (2 papers)

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Research

13 pages, 1448 KiB  
Article
Phenolic Profile of Castanea Bee Pollen from the Northwest of the Iberian Peninsula
by María Shantal Rodríguez-Flores, Olga Escuredo, María Carmen Seijo, Sergio Rojo, Miguel Vilas-Boas and Soraia I. Falcão
Separations 2023, 10(4), 270; https://0-doi-org.brum.beds.ac.uk/10.3390/separations10040270 - 21 Apr 2023
Cited by 7 | Viewed by 1452
Abstract
Bee pollen is a rich bee product, from the point of view of its nutritional and functional chemical characteristics. The chemical composition of bee pollen and its properties make this product an excellent food supplement for the human diet, due to its various [...] Read more.
Bee pollen is a rich bee product, from the point of view of its nutritional and functional chemical characteristics. The chemical composition of bee pollen and its properties make this product an excellent food supplement for the human diet, due to its various functional bioactivities, such as having antioxidant, antibacterial, antifungal, and anti-inflammatory properties. These properties depend on the botanical origin of the bee pollen. Castanea sativa bee pollen is one of the most important types of pollen collected in the northwest of the Iberian Peninsula. Thus, the phenolic profile of Castanea bee pollen was featured in this study. For this, 11 samples of Castanea were selected through prior colorimetric separation using the CIELab* scale and verified with palynological analysis. Identification of the main phenol compounds was performed through LC/DAD/ESI-MSn analysis. The phenols compounds were quantified using calibration curves for caffeic acid, quercetin, and naringenin. The main results showed a profile formed of 19 compounds for all samples, although quantitative differences were found. Most of these compounds were phenolamides, with N1, N5, and N10-tricaffeoylspermidine being significantly (p < 0.05) the most abundant. Three isorhamnetin glycoside derivatives and one naringenin were also identified. The richness in phenolamides of Castanea bee pollen identified in this study suggests Castanea bee pollen as a functional food, owing to its healthy properties. Full article
(This article belongs to the Special Issue Chemical and Biological Technology Applied in Natural Food Products Analysis)
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17 pages, 2081 KiB  
Article
In Vitro Cytotoxicity and Spectral Analysis-Based Phytochemical Profiling of Methanol Extract of Barleria hochstetteri, and Molecular Mechanisms Underlying Its Apoptosis-Inducing Effect on Breast and Lung Cancer Cell Lines
by Saad Ahmed Alkahtani, Ali Mohamed Alshabi, Ibrahim Ahmed Shaikh, Mohamed A. A. Orabi, Basel A. Abdel-Wahab, Ismail A. Walbi, Mohammed Shafiuddin Habeeb, Masood Medleri Khateeb, Arun K. Shettar and Joy H. Hoskeri
Separations 2022, 9(10), 298; https://doi.org/10.3390/separations9100298 - 09 Oct 2022
Cited by 8 | Viewed by 2339
Abstract
The objectives of this research were to carry out GC–MS and LC–MS-based phytochemical profiling of Barleria hochstetteri, as well as flow cytometry-based mechanistic investigations of the cytotoxic effect of its extracts against breast and lung cancer cell lines. This preclinical in vitro [...] Read more.
The objectives of this research were to carry out GC–MS and LC–MS-based phytochemical profiling of Barleria hochstetteri, as well as flow cytometry-based mechanistic investigations of the cytotoxic effect of its extracts against breast and lung cancer cell lines. This preclinical in vitro study was carried out in Saudi Arabia and India, from 11 August to 15 January 2022. Barleria hochstetteri was sequentially extracted using the Soxhlet extraction technique. Utilizing LC–MS and GC–MS methods, the phytochemical profiling was performed. Additionally, the total phenolic compounds and flavonoids were quantified in the plant extract using spectrophotometric techniques. In this study, we first examined the cytotoxicity of the plant extract on non-malignant L929 cells and on the carcinogenic MCF-7 and A549 cell lines. Then, we studied the underlying molecular pathways by means of Anti-Bcl-2, caspase-3, and DNA fragmentation (TUNEL) assays, using flow cytometry. The results revealed phenolic compounds and flavonoids to be the two major components in the methanolic extract of B. hochstetteri, with concentrations of 3210 µg GAE/g dwt and 1863 µg QE/g dwt, respectively. Results from GC–MS and LC–MS analyses revealed the presence of bioactive phytochemicals with known cytotoxicity. From the MTT assay on cell viability, the IC50 of the methanol extract for the MCF-7 and A549 cell lines were 219.67 and 144.30 µg/mL, respectively. With IC50 values of 324.24 and 266.66 µg/mL, respectively, the aqueous and methanol extracts were less toxic when tested against the non-cancerous L929 cell line. The extract caused early and late apoptosis in the tested breast and lung cancer cells by activating caspase-3 and inhibiting Bcl-2 protein, and it also caused cell death via DNA damage, based on flow cytometric and molecular marker analyses. These findings indicate that the methanol extract of B. hochstetteri was cytotoxic on breast cancer and lung cancer cell lines. To uncover cancer-fighting chemicals, there is a need for further research on B. hochstetteri, as it is a promising source of anti-cancer chemotherapeutic drugs. Full article
(This article belongs to the Special Issue Chemical and Biological Technology Applied in Natural Food Products Analysis)
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