Applications of the Zebrafish Model

Topic Information

Dear Colleagues,

Zebrafish has become an increasingly important model organsim for studying a wide variety of biological processes. Since being identified as a genetic model animal in the 1980s, it has been used for deciphering developmental mechanisms with great success. Importantly, more than 70% of human genes have at least one zebrafish orthologue, and 84% of human disease genes have an equivalent in zebrafish. Therefore, the application of zebrafish model in biomedical researches has rapidly expanded over the past two decades. This Topic welcomes submissions of original research and review manuscripts focusing on the application of zebrafish model for understanding molecular and cellular mechanisms underlying physiological and pathological processes. It covers large aspects of studies by taking advantage of the zebrafish model, including but not limited to early development, morphogenesis, organogenesis, tissue regeneration, neural function, reproduction, behaviors, disease modeling, drug screening, toxicology, live Imaging, multiomics and gene mutations by genome-editing approach. The aim is to provide an overview of the strong potential in applying the zebrafish model for understanding development and disease.

Dr. De-Li Shi
Dr. Pengfei Xu
Topic Editors

Keywords

Participating Journals

Journal Name	Impact Factor	CiteScore	Launched Year	First Decision (median)	APC
Biology biology	4.2	4.0	2012	18.7 Days	CHF 2700	Submit
BioMed biomed	-	-	2021	27 Days	CHF 1000	Submit
Cells cells	6.0	9.0	2012	16.6 Days	CHF 2700	Submit
Genes genes	3.5	5.1	2010	16.5 Days	CHF 2600	Submit

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Published Papers (2 papers)

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Open AccessArticle

Evaluation of Tacrolimus’ Adverse Effects on Zebrafish in Larval and Adult Stages by Using Multiple Physiological and Behavioral Endpoints

by Wen-Wei Feng, Hsiu-Chao Chen, Gilbert Audira, Michael Edbert Suryanto, Ferry Saputra, Kevin Adi Kurnia, Ross D. Vasquez, Franelyne P. Casuga, Yu-Heng Lai, Chung-Der Hsiao and Chih-Hsin Hung

Biology 2024, 13(2), 112; https://0-doi-org.brum.beds.ac.uk/10.3390/biology13020112 - 10 Feb 2024

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Abstract

Tacrolimus (FK506) is a common immunosuppressant that is used in organ transplantation. However, despite its importance in medical applications, it is prone to adverse side effects. While some studies have demonstrated its toxicities to humans and various animal models, very few studies have [...] Read more.

Tacrolimus (FK506) is a common immunosuppressant that is used in organ transplantation. However, despite its importance in medical applications, it is prone to adverse side effects. While some studies have demonstrated its toxicities to humans and various animal models, very few studies have addressed this issue in aquatic organisms, especially zebrafish. Here, we assessed the adverse effects of acute and chronic exposure to tacrolimus in relatively low doses in zebrafish in both larval and adult stages, respectively. Based on the results, although tacrolimus did not cause any cardiotoxicity and respiratory toxicity toward zebrafish larvae, it affected their locomotor activity performance in light–dark locomotion tests. Meanwhile, tacrolimus was also found to slightly affect the behavior performance, shoaling formation, circadian rhythm locomotor activity, and color preference of adult zebrafish in a dose-dependent manner. In addition, alterations in the cognitive performance of the fish were also displayed by the treated fish, indicated by a loss of short-term memory. To help elucidate the toxicity mechanism of tacrolimus, molecular docking was conducted to calculate the strength of the binding interaction between tacrolimus to human FKBP12. The results showed a relatively normal binding affinity, indicating that this interaction might only partly contribute to the observed alterations. Nevertheless, the current research could help clinicians and researchers to further understand the toxicology of tacrolimus, especially to zebrafish, thus highlighting the importance of considering the toxicity of tacrolimus prior to its usage. Full article

(This article belongs to the Topic Applications of the Zebrafish Model)

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18 pages, 2112 KiB  

Open AccessArticle

The Effects of Aging on Rod Bipolar Cell Ribbon Synapses

by Abhishek P. Shrestha, Nirujan Rameshkumar, Johane M. Boff, Rhea Rajmanna, Thadshayini Chandrasegaran, Courtney E. Frederick, David Zenisek and Thirumalini Vaithianathan

Cells 2023, 12(19), 2385; https://0-doi-org.brum.beds.ac.uk/10.3390/cells12192385 - 29 Sep 2023

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Abstract

The global health concern posed by age-related visual impairment highlights the need for further research focused on the visual changes that occur during the process of aging. To date, multiple sensory alterations related to aging have been identified, including morphological and functional changes [...] Read more.

The global health concern posed by age-related visual impairment highlights the need for further research focused on the visual changes that occur during the process of aging. To date, multiple sensory alterations related to aging have been identified, including morphological and functional changes in inner hair cochlear cells, photoreceptors, and retinal ganglion cells. While some age-related morphological changes are known to occur in rod bipolar cells in the retina, their effects on these cells and on their connection to other cells via ribbon synapses remain elusive. To investigate the effects of aging on rod bipolar cells and their ribbon synapses, we compared synaptic calcium currents, calcium dynamics, and exocytosis in zebrafish (Danio rerio) that were middle-aged (MA,18 months) or old-aged (OA, 36 months). The bipolar cell terminal in OA zebrafish exhibited a two-fold reduction in number of synaptic ribbons, an increased ribbon length, and a decrease in local Ca²⁺ signals at the tested ribbon location, with little change in the overall magnitude of the calcium current or exocytosis in response to brief pulses. Staining of the synaptic ribbons with antibodies specific for PKCa revealed shortening of the inner nuclear and plexiform layers (INL and IPL). These findings shed light on age-related changes in the retina that are related to synaptic ribbons and calcium signals. Full article

(This article belongs to the Topic Applications of the Zebrafish Model)

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